Radio-protective effects of ultra-fine bubble hydrogen water and serum protein responses in whole-body radiation-exposed mice
Abstract Many studies have demonstrated hydrogen’s therapeutic and preventive effects on various diseases. Its selective antioxidant properties against hydroxyl radicals, which are responsible for the indirect effects of ionizing radiation, may make it worthy of attention as a new radio-protector. W...
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Nature Portfolio
2025-02-01
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| Online Access: | https://doi.org/10.1038/s41598-025-87963-z |
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| author | Masaru Yamaguchi Khin Thandar Htun Youta Tatara Yoshiaki Sato Masato Hosoda Suchart Kothan Chikashi Kamimura Osamu Inanami Ikuo Kashiwakura |
| author_facet | Masaru Yamaguchi Khin Thandar Htun Youta Tatara Yoshiaki Sato Masato Hosoda Suchart Kothan Chikashi Kamimura Osamu Inanami Ikuo Kashiwakura |
| author_sort | Masaru Yamaguchi |
| collection | DOAJ |
| description | Abstract Many studies have demonstrated hydrogen’s therapeutic and preventive effects on various diseases. Its selective antioxidant properties against hydroxyl radicals, which are responsible for the indirect effects of ionizing radiation, may make it worthy of attention as a new radio-protector. We recently developed new hydrogen water that is more stable and has higher antioxidant activity by using ultra-fine bubbles. In this study, female C57BL/6J mice given ad libitum access to ultra-fine bubble hydrogen water (UBHW) were subjected to whole-body irradiation (WBI) with X-rays, and the radio-protective effect of UBHW was evaluated. WBI with 6.0 Gy (sub-lethal dose) resulted in a 30-day survival rate of 100% in UBHW-fed mice, compared with 37% in control mice. In the case of WBI with 6.5 Gy (lethal dose), while the control mice died out in about 3 weeks, the 30-day survival rate improved to 40% by UBHW due to the high scavenging activity of hydroxy radicals. Twenty-six serum proteins involved in inflammatory and immune responses were significantly identified in UBHW-fed mice by proteomics, and UBHW may enhance and regulate these functions, resulting in reduced damage in mice exposed to WBI. We conclude that UBHW has good potential in radio-protection, with evidence that warrants further research efforts in this field. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-3c041d72cdd44cd2a3df05d3a021c6ff2025-02-09T12:32:12ZengNature PortfolioScientific Reports2045-23222025-02-0115111110.1038/s41598-025-87963-zRadio-protective effects of ultra-fine bubble hydrogen water and serum protein responses in whole-body radiation-exposed miceMasaru Yamaguchi0Khin Thandar Htun1Youta Tatara2Yoshiaki Sato3Masato Hosoda4Suchart Kothan5Chikashi Kamimura6Osamu Inanami7Ikuo Kashiwakura8Graduate School of Health Sciences, Hirosaki UniversityCenter of Radiation Research and Medical Imaging, Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityGraduate School of Medicine, Hirosaki UniversityNorris Comprehensive Cancer Center, University of Southern CaliforniaInterprotein CorporationCenter of Radiation Research and Medical Imaging, Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityInformation Science Research Institute LtdLaboratory of Radiation Biology, Department of Applied Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido UniversityGraduate School of Health Sciences, Hirosaki UniversityAbstract Many studies have demonstrated hydrogen’s therapeutic and preventive effects on various diseases. Its selective antioxidant properties against hydroxyl radicals, which are responsible for the indirect effects of ionizing radiation, may make it worthy of attention as a new radio-protector. We recently developed new hydrogen water that is more stable and has higher antioxidant activity by using ultra-fine bubbles. In this study, female C57BL/6J mice given ad libitum access to ultra-fine bubble hydrogen water (UBHW) were subjected to whole-body irradiation (WBI) with X-rays, and the radio-protective effect of UBHW was evaluated. WBI with 6.0 Gy (sub-lethal dose) resulted in a 30-day survival rate of 100% in UBHW-fed mice, compared with 37% in control mice. In the case of WBI with 6.5 Gy (lethal dose), while the control mice died out in about 3 weeks, the 30-day survival rate improved to 40% by UBHW due to the high scavenging activity of hydroxy radicals. Twenty-six serum proteins involved in inflammatory and immune responses were significantly identified in UBHW-fed mice by proteomics, and UBHW may enhance and regulate these functions, resulting in reduced damage in mice exposed to WBI. We conclude that UBHW has good potential in radio-protection, with evidence that warrants further research efforts in this field.https://doi.org/10.1038/s41598-025-87963-zUltra-fine bubble hydrogen waterWhole body irradiationRadio-protective effectsProteome analysisScavenging ability against hydroxy radicals |
| spellingShingle | Masaru Yamaguchi Khin Thandar Htun Youta Tatara Yoshiaki Sato Masato Hosoda Suchart Kothan Chikashi Kamimura Osamu Inanami Ikuo Kashiwakura Radio-protective effects of ultra-fine bubble hydrogen water and serum protein responses in whole-body radiation-exposed mice Scientific Reports Ultra-fine bubble hydrogen water Whole body irradiation Radio-protective effects Proteome analysis Scavenging ability against hydroxy radicals |
| title | Radio-protective effects of ultra-fine bubble hydrogen water and serum protein responses in whole-body radiation-exposed mice |
| title_full | Radio-protective effects of ultra-fine bubble hydrogen water and serum protein responses in whole-body radiation-exposed mice |
| title_fullStr | Radio-protective effects of ultra-fine bubble hydrogen water and serum protein responses in whole-body radiation-exposed mice |
| title_full_unstemmed | Radio-protective effects of ultra-fine bubble hydrogen water and serum protein responses in whole-body radiation-exposed mice |
| title_short | Radio-protective effects of ultra-fine bubble hydrogen water and serum protein responses in whole-body radiation-exposed mice |
| title_sort | radio protective effects of ultra fine bubble hydrogen water and serum protein responses in whole body radiation exposed mice |
| topic | Ultra-fine bubble hydrogen water Whole body irradiation Radio-protective effects Proteome analysis Scavenging ability against hydroxy radicals |
| url | https://doi.org/10.1038/s41598-025-87963-z |
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