Characterization, Molecular Identification and Antimicrobial Susceptibility Testing of Diarrheagenic Escherichia coli Isolated from Diarrhea Infected Patients Around Dhaka City
Background: Mostly Escherichia coli is an innocuous intestinal inhabitant and does not cause infection or disease in a healthy individual. Nevertheless, Shiga toxigenic Escherichia coli (STEC) is one of the few strains that causes severe diarrheal disease in humans all over the world, where tropica...
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Jinnah University for Women
2025-02-01
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| Series: | RADS Journal of Biological Research & Applied Science |
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| Online Access: | https://jbas.juw.edu.pk/index.php/JBAS/article/view/652 |
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| author | Fahima Akter Md. Shamimuzzaman Susmita Roy Chowdhury Faria Islam Rajib Kanti Roy |
| author_facet | Fahima Akter Md. Shamimuzzaman Susmita Roy Chowdhury Faria Islam Rajib Kanti Roy |
| author_sort | Fahima Akter |
| collection | DOAJ |
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Background: Mostly Escherichia coli is an innocuous intestinal inhabitant and does not cause infection or disease in a healthy individual. Nevertheless, Shiga toxigenic Escherichia coli (STEC) is one of the few strains that causes severe diarrheal disease in humans all over the world, where tropical and developing countries have a higher incident rate.
Objective: This research aimed to determine and evaluate the vulnerability of diarrheagenic E. coli to certain antibiotics isolated from hospitalized diarrhea-infected patients in Dhaka city.
Methodology: After initial cultural isolation and identification through BAM, E. coli species were identified automatically via VITEK 2 COMPACT ID-GN card. Molecular identification was performed through detection of 16S rRNA, stx1, and stx2 genes by means of PCR. VITEK 2 COMPACT was used to perform the Antimicrobial Susceptibility Test (AST). Agreement and error were analyzed based on Clinical & Laboratory Standards Institute (CLSI) guidelines.
Results: With the VITEK 2 COMPACT system, 218 (97.75%) of the 223 isolates were accurately identified to the species level. 32.7% (73 out of 218) of the isolates in the research we conducted were classified as very good, and 45.7% (102 out of 218) of the isolates were recognized at the excellent level. The percentages of individuals with good and acceptable level identification were 10.8% (24 out of 218) and 8.5% (19 out of 218), respectively. More importantly, 2.2% (5 out of 218) isolates were unidentified by the system. In this experiment, from 218 isolates of E. coli, stx1 and stx2 were detected in 86 (39.4%) and 122 (56.0%), respectively. In total, 218 organisms were tested for antibiograms with AST-GN72 card after identification. We found that 81.6 % of isolated strains (178 out of 218) exerted at least resistance to one antibiotic.
Conclusion: Multi-resistant serovars most frequently showed resistance to ampicillin, chloramphenicol, trimethoprim, and sulphonamide. Ongoing research is an evidence for researchers to evaluate the etiology of emerging multi-drug resistance Shiga-toxin producing E. coli.
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| format | Article |
| id | doaj-art-3c01c4c7f6174a7ab1447a4a4c0a1507 |
| institution | Kabale University |
| issn | 2305-8722 2521-8573 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Jinnah University for Women |
| record_format | Article |
| series | RADS Journal of Biological Research & Applied Science |
| spelling | doaj-art-3c01c4c7f6174a7ab1447a4a4c0a15072025-02-10T07:39:39ZengJinnah University for WomenRADS Journal of Biological Research & Applied Science2305-87222521-85732025-02-01151Characterization, Molecular Identification and Antimicrobial Susceptibility Testing of Diarrheagenic Escherichia coli Isolated from Diarrhea Infected Patients Around Dhaka CityFahima Akter0Md. Shamimuzzaman1Susmita Roy Chowdhury2Faria Islam3Rajib Kanti Roy4Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.National Drug Control Laboratory, Directorate General of Drug Administration, Ministry of Health and Family Welfare, Dhaka, Bangladesh.Department of Microbiology, Jashore University of Science and Technology, Jashore 7408, Bangladesh.Department of Microbiology, Jashore University of Science and Technology, Jashore 7408, Bangladesh.Department of Nutrition and Food Technology, Jashore University of Science and Technology, Jashore 7408, Bangladesh. Background: Mostly Escherichia coli is an innocuous intestinal inhabitant and does not cause infection or disease in a healthy individual. Nevertheless, Shiga toxigenic Escherichia coli (STEC) is one of the few strains that causes severe diarrheal disease in humans all over the world, where tropical and developing countries have a higher incident rate. Objective: This research aimed to determine and evaluate the vulnerability of diarrheagenic E. coli to certain antibiotics isolated from hospitalized diarrhea-infected patients in Dhaka city. Methodology: After initial cultural isolation and identification through BAM, E. coli species were identified automatically via VITEK 2 COMPACT ID-GN card. Molecular identification was performed through detection of 16S rRNA, stx1, and stx2 genes by means of PCR. VITEK 2 COMPACT was used to perform the Antimicrobial Susceptibility Test (AST). Agreement and error were analyzed based on Clinical & Laboratory Standards Institute (CLSI) guidelines. Results: With the VITEK 2 COMPACT system, 218 (97.75%) of the 223 isolates were accurately identified to the species level. 32.7% (73 out of 218) of the isolates in the research we conducted were classified as very good, and 45.7% (102 out of 218) of the isolates were recognized at the excellent level. The percentages of individuals with good and acceptable level identification were 10.8% (24 out of 218) and 8.5% (19 out of 218), respectively. More importantly, 2.2% (5 out of 218) isolates were unidentified by the system. In this experiment, from 218 isolates of E. coli, stx1 and stx2 were detected in 86 (39.4%) and 122 (56.0%), respectively. In total, 218 organisms were tested for antibiograms with AST-GN72 card after identification. We found that 81.6 % of isolated strains (178 out of 218) exerted at least resistance to one antibiotic. Conclusion: Multi-resistant serovars most frequently showed resistance to ampicillin, chloramphenicol, trimethoprim, and sulphonamide. Ongoing research is an evidence for researchers to evaluate the etiology of emerging multi-drug resistance Shiga-toxin producing E. coli. https://jbas.juw.edu.pk/index.php/JBAS/article/view/652Automatic identificationVITEK 2 COMPACT ID & AST cardShiga toxinstx genes16S rRNAPolymerase chain reaction |
| spellingShingle | Fahima Akter Md. Shamimuzzaman Susmita Roy Chowdhury Faria Islam Rajib Kanti Roy Characterization, Molecular Identification and Antimicrobial Susceptibility Testing of Diarrheagenic Escherichia coli Isolated from Diarrhea Infected Patients Around Dhaka City RADS Journal of Biological Research & Applied Science Automatic identification VITEK 2 COMPACT ID & AST card Shiga toxin stx genes 16S rRNA Polymerase chain reaction |
| title | Characterization, Molecular Identification and Antimicrobial Susceptibility Testing of Diarrheagenic Escherichia coli Isolated from Diarrhea Infected Patients Around Dhaka City |
| title_full | Characterization, Molecular Identification and Antimicrobial Susceptibility Testing of Diarrheagenic Escherichia coli Isolated from Diarrhea Infected Patients Around Dhaka City |
| title_fullStr | Characterization, Molecular Identification and Antimicrobial Susceptibility Testing of Diarrheagenic Escherichia coli Isolated from Diarrhea Infected Patients Around Dhaka City |
| title_full_unstemmed | Characterization, Molecular Identification and Antimicrobial Susceptibility Testing of Diarrheagenic Escherichia coli Isolated from Diarrhea Infected Patients Around Dhaka City |
| title_short | Characterization, Molecular Identification and Antimicrobial Susceptibility Testing of Diarrheagenic Escherichia coli Isolated from Diarrhea Infected Patients Around Dhaka City |
| title_sort | characterization molecular identification and antimicrobial susceptibility testing of diarrheagenic escherichia coli isolated from diarrhea infected patients around dhaka city |
| topic | Automatic identification VITEK 2 COMPACT ID & AST card Shiga toxin stx genes 16S rRNA Polymerase chain reaction |
| url | https://jbas.juw.edu.pk/index.php/JBAS/article/view/652 |
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