Functional potential of intermediate wheatgrass (IWG) in bread formulations: role of base flour gluten strength

Intermediate wheatgrass (IWG, Thinopyrum intermedium), a perennial grain rich in dietary fiber, protein, and antioxidants, considered as a functional ingredient for nutritionally enhanced baked goods. However, its limited use in breadmaking is due to weak gluten formation associated with high fiber...

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Main Authors: Md Ahmadul Islam, Jayani Kulathunga, Yun Zhao, Shahidul Islam
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:Applied Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772502225005517
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author Md Ahmadul Islam
Jayani Kulathunga
Yun Zhao
Shahidul Islam
author_facet Md Ahmadul Islam
Jayani Kulathunga
Yun Zhao
Shahidul Islam
author_sort Md Ahmadul Islam
collection DOAJ
description Intermediate wheatgrass (IWG, Thinopyrum intermedium), a perennial grain rich in dietary fiber, protein, and antioxidants, considered as a functional ingredient for nutritionally enhanced baked goods. However, its limited use in breadmaking is due to weak gluten formation associated with high fiber content. While base flour gluten strength is crucial in determining the allowable level of IWG flour without compromising bread quality, systematic comparisons across flours with varying gluten strengths are limited. This study evaluates IWG flour as a partial substitute in three wheat flours: all-purpose (AP, medium gluten), whole wheat flour (WWF, medium-low gluten), and hard red spring (HRS, high gluten), to determine optimal inclusion levels that preserve quality and enhance nutrition. Blends with 10 %, 20 %, and 30 % IWG flour were assessed for proximate composition, protein and starch functionality, dietary fiber, total phenolic content, antioxidant activity, dough rheology, and bread quality. Despite its higher protein content (22.65 %), IWG showed the lowest unextractable polymeric protein, aggregation energy, and HMW glutenins. Base flours varied in their ability to incorporate IWG: HRS supported up to 20 % and AP up to 10 % without adversely affecting dough or bread quality. These levels significantly improved bread nutrition: fiber increased from 3.20 to 4.35 % and 2.95 to 3.65 %; phenolics from 18.73 to 19.96 % and 12.34 to 16.11 %; and antioxidant activity from 4.37 to 8.07 and 2.51 to 4.67 mg Trolox/100 g, respectively. WWF was not suitable for IWG inclusion. Findings highlight the importance of base flour gluten strength in leveraging IWG's health benefits without compromising bread quality.
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spelling doaj-art-3bf225799fb94d2bb1ed45b244249a962025-08-20T04:01:57ZengElsevierApplied Food Research2772-50222025-12-015210124610.1016/j.afres.2025.101246Functional potential of intermediate wheatgrass (IWG) in bread formulations: role of base flour gluten strengthMd Ahmadul Islam0Jayani Kulathunga1Yun Zhao2Shahidul Islam3Department of Plant Sciences, North Dakota State University. Fargo, ND 58108-6050, USA; Department of Food Engineering and Technology, Bangladesh Agricultural University, Mymensingh-2202, BangladeshDepartment of Plant Sciences, North Dakota State University. Fargo, ND 58108-6050, USA; Department of Multidisciplinary Studies, Faculty of Urban and Aquatic Bioresources, University of Sri Jayewardenepura, Sri LankaDepartment of Plant Sciences, North Dakota State University. Fargo, ND 58108-6050, USADepartment of Plant Sciences, North Dakota State University. Fargo, ND 58108-6050, USA; Centre for Crop and Food Innovation, Food Futures Institute, Murdoch University, WA-6150, Australia; Corresponding author at: Department of Plant Sciences, North Dakota State University. Fargo, ND 58108-6050, USA.Intermediate wheatgrass (IWG, Thinopyrum intermedium), a perennial grain rich in dietary fiber, protein, and antioxidants, considered as a functional ingredient for nutritionally enhanced baked goods. However, its limited use in breadmaking is due to weak gluten formation associated with high fiber content. While base flour gluten strength is crucial in determining the allowable level of IWG flour without compromising bread quality, systematic comparisons across flours with varying gluten strengths are limited. This study evaluates IWG flour as a partial substitute in three wheat flours: all-purpose (AP, medium gluten), whole wheat flour (WWF, medium-low gluten), and hard red spring (HRS, high gluten), to determine optimal inclusion levels that preserve quality and enhance nutrition. Blends with 10 %, 20 %, and 30 % IWG flour were assessed for proximate composition, protein and starch functionality, dietary fiber, total phenolic content, antioxidant activity, dough rheology, and bread quality. Despite its higher protein content (22.65 %), IWG showed the lowest unextractable polymeric protein, aggregation energy, and HMW glutenins. Base flours varied in their ability to incorporate IWG: HRS supported up to 20 % and AP up to 10 % without adversely affecting dough or bread quality. These levels significantly improved bread nutrition: fiber increased from 3.20 to 4.35 % and 2.95 to 3.65 %; phenolics from 18.73 to 19.96 % and 12.34 to 16.11 %; and antioxidant activity from 4.37 to 8.07 and 2.51 to 4.67 mg Trolox/100 g, respectively. WWF was not suitable for IWG inclusion. Findings highlight the importance of base flour gluten strength in leveraging IWG's health benefits without compromising bread quality.http://www.sciencedirect.com/science/article/pii/S2772502225005517Baking qualityGlutenIntermediate wheat grassWholegrainNutrition
spellingShingle Md Ahmadul Islam
Jayani Kulathunga
Yun Zhao
Shahidul Islam
Functional potential of intermediate wheatgrass (IWG) in bread formulations: role of base flour gluten strength
Applied Food Research
Baking quality
Gluten
Intermediate wheat grass
Wholegrain
Nutrition
title Functional potential of intermediate wheatgrass (IWG) in bread formulations: role of base flour gluten strength
title_full Functional potential of intermediate wheatgrass (IWG) in bread formulations: role of base flour gluten strength
title_fullStr Functional potential of intermediate wheatgrass (IWG) in bread formulations: role of base flour gluten strength
title_full_unstemmed Functional potential of intermediate wheatgrass (IWG) in bread formulations: role of base flour gluten strength
title_short Functional potential of intermediate wheatgrass (IWG) in bread formulations: role of base flour gluten strength
title_sort functional potential of intermediate wheatgrass iwg in bread formulations role of base flour gluten strength
topic Baking quality
Gluten
Intermediate wheat grass
Wholegrain
Nutrition
url http://www.sciencedirect.com/science/article/pii/S2772502225005517
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AT yunzhao functionalpotentialofintermediatewheatgrassiwginbreadformulationsroleofbaseflourglutenstrength
AT shahidulislam functionalpotentialofintermediatewheatgrassiwginbreadformulationsroleofbaseflourglutenstrength