In Vitro Assessment of Antifungal Caspofungin on Leishmania donovani Culture Isolation
Leishmania parasite isolation from the human aspirates is always challenging due to most probability of the fungal contamination and the use of antifungal drug which could support the selective growth of the Leishmania parasite. In this study, we examine the effect of antifungal drug caspofungin on...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2017/3873187 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556267281842176 |
|---|---|
| author | Narayan Raj Bhattarai Keshav Rai Suman Rijal Basudha Khanal |
| author_facet | Narayan Raj Bhattarai Keshav Rai Suman Rijal Basudha Khanal |
| author_sort | Narayan Raj Bhattarai |
| collection | DOAJ |
| description | Leishmania parasite isolation from the human aspirates is always challenging due to most probability of the fungal contamination and the use of antifungal drug which could support the selective growth of the Leishmania parasite. In this study, we examine the effect of antifungal drug caspofungin on the promastigote stage of Leishmania donovani. Promastigote parasite was cultivated in M199 + 20% heat-inactivated fetal calf serum and plated in 96-well plates. Seven different concentrations of caspofungin (512 µg/ml to 8 µg/ml) were exposed to parasites, and 50% inhibitory concentration (IC50) was calculated. Candida spp. was used in the experiments to know the efficacy of caspofungin to inhibit fungal growth. The IC50 values of Leishmania strains ranged from 23.02 to 155.80 µg/ml (mean 90.25 ± 39.01 µg/ml), and it was significantly higher (P value = 0.02) than IC50 values of Candida spp. (ranged from 0.001 to 0.12 µg/ml, mean = 0.05 ± 0.05 µg/ml). The reduced growth rate of the parasite was found with exposure to 50 µg/ml of caspofungin. Growth inhibition of Leishmania donovani is significantly lower with caspofungin and could be used to protect the parasite cultivation from fungal contamination. |
| format | Article |
| id | doaj-art-3bec441bd65c4f4a8c85acf9e5a3a713 |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-3bec441bd65c4f4a8c85acf9e5a3a7132025-02-03T05:45:51ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932017-01-01201710.1155/2017/38731873873187In Vitro Assessment of Antifungal Caspofungin on Leishmania donovani Culture IsolationNarayan Raj Bhattarai0Keshav Rai1Suman Rijal2Basudha Khanal3Department of Microbiology, B. P. Koirala Institute of Health Sciences, Dharan, NepalDepartment of Microbiology, B. P. Koirala Institute of Health Sciences, Dharan, NepalDepartment of Internal Medicine, B. P. Koirala Institute of Health Sciences, Dharan, NepalDepartment of Microbiology, B. P. Koirala Institute of Health Sciences, Dharan, NepalLeishmania parasite isolation from the human aspirates is always challenging due to most probability of the fungal contamination and the use of antifungal drug which could support the selective growth of the Leishmania parasite. In this study, we examine the effect of antifungal drug caspofungin on the promastigote stage of Leishmania donovani. Promastigote parasite was cultivated in M199 + 20% heat-inactivated fetal calf serum and plated in 96-well plates. Seven different concentrations of caspofungin (512 µg/ml to 8 µg/ml) were exposed to parasites, and 50% inhibitory concentration (IC50) was calculated. Candida spp. was used in the experiments to know the efficacy of caspofungin to inhibit fungal growth. The IC50 values of Leishmania strains ranged from 23.02 to 155.80 µg/ml (mean 90.25 ± 39.01 µg/ml), and it was significantly higher (P value = 0.02) than IC50 values of Candida spp. (ranged from 0.001 to 0.12 µg/ml, mean = 0.05 ± 0.05 µg/ml). The reduced growth rate of the parasite was found with exposure to 50 µg/ml of caspofungin. Growth inhibition of Leishmania donovani is significantly lower with caspofungin and could be used to protect the parasite cultivation from fungal contamination.http://dx.doi.org/10.1155/2017/3873187 |
| spellingShingle | Narayan Raj Bhattarai Keshav Rai Suman Rijal Basudha Khanal In Vitro Assessment of Antifungal Caspofungin on Leishmania donovani Culture Isolation Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | In Vitro Assessment of Antifungal Caspofungin on Leishmania donovani Culture Isolation |
| title_full | In Vitro Assessment of Antifungal Caspofungin on Leishmania donovani Culture Isolation |
| title_fullStr | In Vitro Assessment of Antifungal Caspofungin on Leishmania donovani Culture Isolation |
| title_full_unstemmed | In Vitro Assessment of Antifungal Caspofungin on Leishmania donovani Culture Isolation |
| title_short | In Vitro Assessment of Antifungal Caspofungin on Leishmania donovani Culture Isolation |
| title_sort | in vitro assessment of antifungal caspofungin on leishmania donovani culture isolation |
| url | http://dx.doi.org/10.1155/2017/3873187 |
| work_keys_str_mv | AT narayanrajbhattarai invitroassessmentofantifungalcaspofunginonleishmaniadonovanicultureisolation AT keshavrai invitroassessmentofantifungalcaspofunginonleishmaniadonovanicultureisolation AT sumanrijal invitroassessmentofantifungalcaspofunginonleishmaniadonovanicultureisolation AT basudhakhanal invitroassessmentofantifungalcaspofunginonleishmaniadonovanicultureisolation |