Morphological and Immunohistochemical Changes in Progressive Postmortem Autolysis of the Murine Brain
In this time series study, the temporal sequences of postmortem changes in brains kept at different temperatures were investigated in different areas of mouse brains. Fixation of tissues kept at different storage temperatures (4 °C, 22 °C, 37 °C) was delayed for four time points (24, 120, 168, 336 h...
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MDPI AG
2024-12-01
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| Online Access: | https://www.mdpi.com/2076-2615/14/24/3676 |
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| author | Francesca Parisi Sara Degl’Innocenti Çağla Aytaş Andrea Pirone Carlo Cantile |
| author_facet | Francesca Parisi Sara Degl’Innocenti Çağla Aytaş Andrea Pirone Carlo Cantile |
| author_sort | Francesca Parisi |
| collection | DOAJ |
| description | In this time series study, the temporal sequences of postmortem changes in brains kept at different temperatures were investigated in different areas of mouse brains. Fixation of tissues kept at different storage temperatures (4 °C, 22 °C, 37 °C) was delayed for four time points (24, 120, 168, 336 h). Histological and immunohistochemical investigations were carried out to determine how postmortem autolysis may affect the cellular morphology and the expression of neural cell epitopes. Results showed that the autolytic changes started earlier in brains at 22 °C and 37 °C and in the grey matter compared to the white matter, with the cerebellum and hippocampus showing the earliest postmortem changes. The cellular antigens were differently affected by the autolytic process overtime: NeuN and Olig2 immunoreactivity was gradually lost at the nuclear site and diffused into the cytoplasm; increased background staining was observed with SMI-32; GFAP showed an increase in immunolabeling, whereas 2F11 immunoreactivity decreased. This study suggests that the morphological analysis and immunohistochemical investigation of the brain tissue could be satisfactorily applied to forensic cases, providing useful data for the estimation of the postmortem interval. |
| format | Article |
| id | doaj-art-3bebfa91ac054a24a7535e99dd7d954e |
| institution | OA Journals |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-3bebfa91ac054a24a7535e99dd7d954e2025-08-20T02:01:01ZengMDPI AGAnimals2076-26152024-12-011424367610.3390/ani14243676Morphological and Immunohistochemical Changes in Progressive Postmortem Autolysis of the Murine BrainFrancesca Parisi0Sara Degl’Innocenti1Çağla Aytaş2Andrea Pirone3Carlo Cantile4Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyGLP Test Facility, San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyDepartment of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, ItalyIn this time series study, the temporal sequences of postmortem changes in brains kept at different temperatures were investigated in different areas of mouse brains. Fixation of tissues kept at different storage temperatures (4 °C, 22 °C, 37 °C) was delayed for four time points (24, 120, 168, 336 h). Histological and immunohistochemical investigations were carried out to determine how postmortem autolysis may affect the cellular morphology and the expression of neural cell epitopes. Results showed that the autolytic changes started earlier in brains at 22 °C and 37 °C and in the grey matter compared to the white matter, with the cerebellum and hippocampus showing the earliest postmortem changes. The cellular antigens were differently affected by the autolytic process overtime: NeuN and Olig2 immunoreactivity was gradually lost at the nuclear site and diffused into the cytoplasm; increased background staining was observed with SMI-32; GFAP showed an increase in immunolabeling, whereas 2F11 immunoreactivity decreased. This study suggests that the morphological analysis and immunohistochemical investigation of the brain tissue could be satisfactorily applied to forensic cases, providing useful data for the estimation of the postmortem interval.https://www.mdpi.com/2076-2615/14/24/3676brainC57BL/6J mouseautolysispostmortem intervalforensic veterinary pathologyimmunohistochemistry |
| spellingShingle | Francesca Parisi Sara Degl’Innocenti Çağla Aytaş Andrea Pirone Carlo Cantile Morphological and Immunohistochemical Changes in Progressive Postmortem Autolysis of the Murine Brain Animals brain C57BL/6J mouse autolysis postmortem interval forensic veterinary pathology immunohistochemistry |
| title | Morphological and Immunohistochemical Changes in Progressive Postmortem Autolysis of the Murine Brain |
| title_full | Morphological and Immunohistochemical Changes in Progressive Postmortem Autolysis of the Murine Brain |
| title_fullStr | Morphological and Immunohistochemical Changes in Progressive Postmortem Autolysis of the Murine Brain |
| title_full_unstemmed | Morphological and Immunohistochemical Changes in Progressive Postmortem Autolysis of the Murine Brain |
| title_short | Morphological and Immunohistochemical Changes in Progressive Postmortem Autolysis of the Murine Brain |
| title_sort | morphological and immunohistochemical changes in progressive postmortem autolysis of the murine brain |
| topic | brain C57BL/6J mouse autolysis postmortem interval forensic veterinary pathology immunohistochemistry |
| url | https://www.mdpi.com/2076-2615/14/24/3676 |
| work_keys_str_mv | AT francescaparisi morphologicalandimmunohistochemicalchangesinprogressivepostmortemautolysisofthemurinebrain AT saradeglinnocenti morphologicalandimmunohistochemicalchangesinprogressivepostmortemautolysisofthemurinebrain AT caglaaytas morphologicalandimmunohistochemicalchangesinprogressivepostmortemautolysisofthemurinebrain AT andreapirone morphologicalandimmunohistochemicalchangesinprogressivepostmortemautolysisofthemurinebrain AT carlocantile morphologicalandimmunohistochemicalchangesinprogressivepostmortemautolysisofthemurinebrain |