Optimisation of parameters for the extraction of β-amylase from sweet potato via liquid biphasic floatation
This study explores the application of liquid biphasic flotation (LBF) for the extraction of β-amylase from sweet potato (Ipomoea batatas), aiming to provide a greener and more sustainable alternative to traditional extraction methods. The LBF method combines solvent sublation with liquid biphasic s...
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Elsevier
2025-02-01
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| Series: | Cleaner Engineering and Technology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666790824001216 |
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| author | Avvari Sai Divya Clement Yek Kee Chee Ng Yan Jer Revathy Sankaran Angela Paul Peter Show Pau Loke |
| author_facet | Avvari Sai Divya Clement Yek Kee Chee Ng Yan Jer Revathy Sankaran Angela Paul Peter Show Pau Loke |
| author_sort | Avvari Sai Divya |
| collection | DOAJ |
| description | This study explores the application of liquid biphasic flotation (LBF) for the extraction of β-amylase from sweet potato (Ipomoea batatas), aiming to provide a greener and more sustainable alternative to traditional extraction methods. The LBF method combines solvent sublation with liquid biphasic systems, offering potential advantages in terms of energy-efficient extraction, reduced environmental impact, and improved resource efficiency. Experimental investigations were conducted to assess the effects of salt concentration, solvent amount, and flotation time on β-amylase extraction efficiency and separation performance. Results indicate that optimal conditions for β-amylase extraction were achieved at a salt concentration of 0.3 g/mL, a solvent amount of 60 mL, and a flotation time of 15 min with a salt/solvent combination of trisodium citrate and tert-butanol. These optimal conditions resulted in an amylase yield of 18.29%, with a separation efficiency of 0.06% and a purification factor of 291.90. Comparative analysis with liquid biphasic systems (LBS) revealed that the LBF method provided higher efficiency and yield, contributing to more sustainable practices in enzyme extraction. The LBF method shows promise due to its improved performance and potential for reducing the environmental footprint of industrial processes, supporting both innovation and environmental sustainability. This research also studied the effect of various parameters on phase formation and separation efficiency, offering a more thorough understanding of the LBF system that can be applied to future studies with broader sustainability goals. |
| format | Article |
| id | doaj-art-3bd722d8d62d46f0a11d47af81eee592 |
| institution | OA Journals |
| issn | 2666-7908 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
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| series | Cleaner Engineering and Technology |
| spelling | doaj-art-3bd722d8d62d46f0a11d47af81eee5922025-08-20T02:34:44ZengElsevierCleaner Engineering and Technology2666-79082025-02-012410084110.1016/j.clet.2024.100841Optimisation of parameters for the extraction of β-amylase from sweet potato via liquid biphasic floatationAvvari Sai Divya0Clement Yek Kee Chee1Ng Yan Jer2Revathy Sankaran3Angela Paul Peter4Show Pau Loke5Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500, Selangor Darul Ehsan, MalaysiaDepartment of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500, Selangor Darul Ehsan, MalaysiaDepartment of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500, Selangor Darul Ehsan, MalaysiaFaculty of Medicine & Health Sciences, UCSI University, Persiaran Springhill, 71010, Port Dickson, Negeri Sembilan, Malaysia; Corresponding author.Faculty of Innovation & Technology, School of Engineering, Taylor's Lakeside Campus, 1, Jalan Taylors, 47500, Subang Jaya, Selangor, Malaysia; Corresponding author.Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Jalan Broga, Semenyih, 43500, Selangor Darul Ehsan, Malaysia; Department of Chemical Engineering, Khalifa University, Shakhbout Bin Sultan St - Zone 1, Abu Dhabi, United Arab EmiratesThis study explores the application of liquid biphasic flotation (LBF) for the extraction of β-amylase from sweet potato (Ipomoea batatas), aiming to provide a greener and more sustainable alternative to traditional extraction methods. The LBF method combines solvent sublation with liquid biphasic systems, offering potential advantages in terms of energy-efficient extraction, reduced environmental impact, and improved resource efficiency. Experimental investigations were conducted to assess the effects of salt concentration, solvent amount, and flotation time on β-amylase extraction efficiency and separation performance. Results indicate that optimal conditions for β-amylase extraction were achieved at a salt concentration of 0.3 g/mL, a solvent amount of 60 mL, and a flotation time of 15 min with a salt/solvent combination of trisodium citrate and tert-butanol. These optimal conditions resulted in an amylase yield of 18.29%, with a separation efficiency of 0.06% and a purification factor of 291.90. Comparative analysis with liquid biphasic systems (LBS) revealed that the LBF method provided higher efficiency and yield, contributing to more sustainable practices in enzyme extraction. The LBF method shows promise due to its improved performance and potential for reducing the environmental footprint of industrial processes, supporting both innovation and environmental sustainability. This research also studied the effect of various parameters on phase formation and separation efficiency, offering a more thorough understanding of the LBF system that can be applied to future studies with broader sustainability goals.http://www.sciencedirect.com/science/article/pii/S2666790824001216Liquid biphasic floatation (LBF)Enzyme extractionβ-AmylaseSweet potatoGreen bioprocess |
| spellingShingle | Avvari Sai Divya Clement Yek Kee Chee Ng Yan Jer Revathy Sankaran Angela Paul Peter Show Pau Loke Optimisation of parameters for the extraction of β-amylase from sweet potato via liquid biphasic floatation Cleaner Engineering and Technology Liquid biphasic floatation (LBF) Enzyme extraction β-Amylase Sweet potato Green bioprocess |
| title | Optimisation of parameters for the extraction of β-amylase from sweet potato via liquid biphasic floatation |
| title_full | Optimisation of parameters for the extraction of β-amylase from sweet potato via liquid biphasic floatation |
| title_fullStr | Optimisation of parameters for the extraction of β-amylase from sweet potato via liquid biphasic floatation |
| title_full_unstemmed | Optimisation of parameters for the extraction of β-amylase from sweet potato via liquid biphasic floatation |
| title_short | Optimisation of parameters for the extraction of β-amylase from sweet potato via liquid biphasic floatation |
| title_sort | optimisation of parameters for the extraction of β amylase from sweet potato via liquid biphasic floatation |
| topic | Liquid biphasic floatation (LBF) Enzyme extraction β-Amylase Sweet potato Green bioprocess |
| url | http://www.sciencedirect.com/science/article/pii/S2666790824001216 |
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