A cell isolation method from Ligusticum chuanxiong Hort. suitable for obtaining high-quality RNA for Smart-seq

A cell isolation method from Ligusticum chuanxiong Hort. suitable for obtaining high-quality RNA for Smart-seq

Abstract Purpose To overcome the risk of cellular damage and RNA degradation caused by high temperatures and cellular damage induced by laser capture microdissection (LCM) during plant single cell or small cell cluster isolation, we developed a rapid and simple method for single-cell separation and...

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Bibliographic Details
Main Authors: Ruoshi Li, Mengmeng Wu, Shunlu Chen, Lan Huang, Can Wang, Zhiyin Yu, Feng Huang, Xiaofen Liu, Nianyin Zhu, Chi Song, Guihua Jiang, Xianmei Yin
Format: Article
Language:English
Published: BMC 2025-08-01
Series:Plant Methods
Subjects:
Laser capture microdissection
Smart-seq
Paraffin section
Cell Isolation
Ligusticum chuanxiong Hort
Online Access:https://doi.org/10.1186/s13007-025-01425-1
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