A Moderate Water Deficit Induces Profound Changes in the Proteome of Developing Maize Ovaries

Water deficit is a major cause of yield loss for maize (<i>Zea mays</i>), leading to ovary abortion when applied at flowering time. To help understand the mechanisms involved in this phenomenon, the proteome response to water deficit has been analysed in developing ovaries at the silk em...

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Main Authors: Thierry Balliau, Mariamawit Ashenafi, Mélisande Blein-Nicolas, Olivier Turc, Michel Zivy, Elodie Marchadier
Format: Article
Language:English
Published: MDPI AG 2024-09-01
Series:Biomolecules
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Online Access:https://www.mdpi.com/2218-273X/14/10/1239
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author Thierry Balliau
Mariamawit Ashenafi
Mélisande Blein-Nicolas
Olivier Turc
Michel Zivy
Elodie Marchadier
author_facet Thierry Balliau
Mariamawit Ashenafi
Mélisande Blein-Nicolas
Olivier Turc
Michel Zivy
Elodie Marchadier
author_sort Thierry Balliau
collection DOAJ
description Water deficit is a major cause of yield loss for maize (<i>Zea mays</i>), leading to ovary abortion when applied at flowering time. To help understand the mechanisms involved in this phenomenon, the proteome response to water deficit has been analysed in developing ovaries at the silk emergence stage and five days later. Differential analysis, abundance pattern clustering and co-expression networks were performed in order to draw a general picture of the proteome changes all along ovary development and under the effect of water deficit. The results show that even mild water deficit has a major impact on ovary proteome, but this impact is very different from a response to stress. A part of the changes can be related to a slowdown of ovary development, while another part cannot. In particular, ovaries submitted to water deficit show an increase in proteins involved in protein biosynthesis and in vesicle transport together with a decrease in proteins involved in amino acid metabolism and proteolysis. According to the functions of increased proteins, the changes may be linked to auxin, brassinosteroids and jasmonate signalling but not abscisic acid.
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spelling doaj-art-3bd228c2d43d4006a0e5d8568f5dd89a2025-08-20T02:11:12ZengMDPI AGBiomolecules2218-273X2024-09-011410123910.3390/biom14101239A Moderate Water Deficit Induces Profound Changes in the Proteome of Developing Maize OvariesThierry Balliau0Mariamawit Ashenafi1Mélisande Blein-Nicolas2Olivier Turc3Michel Zivy4Elodie Marchadier5AgroParisTech, GQE—Le Moulon, PAPPSO, Université Paris-Saclay, INRAE, CNRS, 91190 Gif-sur-Yvette, FranceAgroParisTech, GQE—Le Moulon, PAPPSO, Université Paris-Saclay, INRAE, CNRS, 91190 Gif-sur-Yvette, FranceAgroParisTech, GQE—Le Moulon, PAPPSO, Université Paris-Saclay, INRAE, CNRS, 91190 Gif-sur-Yvette, FranceLEPSE, INRAE, Montpellier SupAgro, Université Montpellier, 34293 Montpellier, FranceAgroParisTech, GQE—Le Moulon, PAPPSO, Université Paris-Saclay, INRAE, CNRS, 91190 Gif-sur-Yvette, FranceAgroParisTech, GQE—Le Moulon, PAPPSO, Université Paris-Saclay, INRAE, CNRS, 91190 Gif-sur-Yvette, FranceWater deficit is a major cause of yield loss for maize (<i>Zea mays</i>), leading to ovary abortion when applied at flowering time. To help understand the mechanisms involved in this phenomenon, the proteome response to water deficit has been analysed in developing ovaries at the silk emergence stage and five days later. Differential analysis, abundance pattern clustering and co-expression networks were performed in order to draw a general picture of the proteome changes all along ovary development and under the effect of water deficit. The results show that even mild water deficit has a major impact on ovary proteome, but this impact is very different from a response to stress. A part of the changes can be related to a slowdown of ovary development, while another part cannot. In particular, ovaries submitted to water deficit show an increase in proteins involved in protein biosynthesis and in vesicle transport together with a decrease in proteins involved in amino acid metabolism and proteolysis. According to the functions of increased proteins, the changes may be linked to auxin, brassinosteroids and jasmonate signalling but not abscisic acid.https://www.mdpi.com/2218-273X/14/10/1239<i>Zea mays</i>water deficitovariesproteome
spellingShingle Thierry Balliau
Mariamawit Ashenafi
Mélisande Blein-Nicolas
Olivier Turc
Michel Zivy
Elodie Marchadier
A Moderate Water Deficit Induces Profound Changes in the Proteome of Developing Maize Ovaries
Biomolecules
<i>Zea mays</i>
water deficit
ovaries
proteome
title A Moderate Water Deficit Induces Profound Changes in the Proteome of Developing Maize Ovaries
title_full A Moderate Water Deficit Induces Profound Changes in the Proteome of Developing Maize Ovaries
title_fullStr A Moderate Water Deficit Induces Profound Changes in the Proteome of Developing Maize Ovaries
title_full_unstemmed A Moderate Water Deficit Induces Profound Changes in the Proteome of Developing Maize Ovaries
title_short A Moderate Water Deficit Induces Profound Changes in the Proteome of Developing Maize Ovaries
title_sort moderate water deficit induces profound changes in the proteome of developing maize ovaries
topic <i>Zea mays</i>
water deficit
ovaries
proteome
url https://www.mdpi.com/2218-273X/14/10/1239
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