In Vitro Antibacterial Activity and Combined Drug Sensitivity of 18 Lichen Species from Cangshan
This study aims to explore the in vitro antibacterial activity of 18 lichen species from Cangshan and the synergistic effect of their natural active products in combination with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). The in vitro antibacterial activity of 18 lichen s...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2024-10-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023110027 |
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| author | Hongqiao TIAN Jiana ZHU Menglong LIU Haiyan DING |
| author_facet | Hongqiao TIAN Jiana ZHU Menglong LIU Haiyan DING |
| author_sort | Hongqiao TIAN |
| collection | DOAJ |
| description | This study aims to explore the in vitro antibacterial activity of 18 lichen species from Cangshan and the synergistic effect of their natural active products in combination with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). The in vitro antibacterial activity of 18 lichen species against 6 food-borne pathogens was determined using the agar well diffusion method, and the combined effect of active lichens and 7 common antibiotics was evaluated using the checkerboard method with MRSA as the tracer. Additionally, in vitro bactericidal curves, anti-mutagenic concentrations, and post-antibiotic effects were examined. The results demonstrated that 10 of the 18 lichen species exhibited in vitro anti-MRSA activity, with strongest antibacterial activity observed for Cetraria laevigata, showing extremely high sensitivity (the diameter of the inhibition zone was 22.0 mm). Furthermore, the combination of Lobaria kurokawae and Cladonia uncialis with piperacillin demonstrated a synergistic antibacterial effect, significantly reducing MRSA bacterial density (P<0.05), enhancing mutation prevention capability, and prolonging the post-antibiotic effect of piperacillin. These findings suggest that the extracts from various Cangshan lichen species exhibit promising antimicrobial activity against MRSA, and the combination of Lobaria kurokawae, Cladonia uncialis, and piperacillin shows a synergistic antibacterial effect, which have the potential for further development as antibiotics or antibiotic adjuvants. |
| format | Article |
| id | doaj-art-3bd01cf7463149658a4d88e3c098657a |
| institution | OA Journals |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2024-10-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj-art-3bd01cf7463149658a4d88e3c098657a2025-08-20T01:54:33ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-10-01451914915710.13386/j.issn1002-0306.20231100272023110027-19In Vitro Antibacterial Activity and Combined Drug Sensitivity of 18 Lichen Species from CangshanHongqiao TIAN0Jiana ZHU1Menglong LIU2Haiyan DING3School of Public Health, Dali University, Dali 671000, ChinaSchool of Public Health, Dali University, Dali 671000, ChinaSchool of Public Health, Dali University, Dali 671000, ChinaSchool of Public Health, Dali University, Dali 671000, ChinaThis study aims to explore the in vitro antibacterial activity of 18 lichen species from Cangshan and the synergistic effect of their natural active products in combination with antibiotics against methicillin-resistant Staphylococcus aureus (MRSA). The in vitro antibacterial activity of 18 lichen species against 6 food-borne pathogens was determined using the agar well diffusion method, and the combined effect of active lichens and 7 common antibiotics was evaluated using the checkerboard method with MRSA as the tracer. Additionally, in vitro bactericidal curves, anti-mutagenic concentrations, and post-antibiotic effects were examined. The results demonstrated that 10 of the 18 lichen species exhibited in vitro anti-MRSA activity, with strongest antibacterial activity observed for Cetraria laevigata, showing extremely high sensitivity (the diameter of the inhibition zone was 22.0 mm). Furthermore, the combination of Lobaria kurokawae and Cladonia uncialis with piperacillin demonstrated a synergistic antibacterial effect, significantly reducing MRSA bacterial density (P<0.05), enhancing mutation prevention capability, and prolonging the post-antibiotic effect of piperacillin. These findings suggest that the extracts from various Cangshan lichen species exhibit promising antimicrobial activity against MRSA, and the combination of Lobaria kurokawae, Cladonia uncialis, and piperacillin shows a synergistic antibacterial effect, which have the potential for further development as antibiotics or antibiotic adjuvants.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023110027yunnan lichenantibiotic activitycombined drug sensitivitysynergistic effect |
| spellingShingle | Hongqiao TIAN Jiana ZHU Menglong LIU Haiyan DING In Vitro Antibacterial Activity and Combined Drug Sensitivity of 18 Lichen Species from Cangshan Shipin gongye ke-ji yunnan lichen antibiotic activity combined drug sensitivity synergistic effect |
| title | In Vitro Antibacterial Activity and Combined Drug Sensitivity of 18 Lichen Species from Cangshan |
| title_full | In Vitro Antibacterial Activity and Combined Drug Sensitivity of 18 Lichen Species from Cangshan |
| title_fullStr | In Vitro Antibacterial Activity and Combined Drug Sensitivity of 18 Lichen Species from Cangshan |
| title_full_unstemmed | In Vitro Antibacterial Activity and Combined Drug Sensitivity of 18 Lichen Species from Cangshan |
| title_short | In Vitro Antibacterial Activity and Combined Drug Sensitivity of 18 Lichen Species from Cangshan |
| title_sort | in vitro antibacterial activity and combined drug sensitivity of 18 lichen species from cangshan |
| topic | yunnan lichen antibiotic activity combined drug sensitivity synergistic effect |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023110027 |
| work_keys_str_mv | AT hongqiaotian invitroantibacterialactivityandcombineddrugsensitivityof18lichenspeciesfromcangshan AT jianazhu invitroantibacterialactivityandcombineddrugsensitivityof18lichenspeciesfromcangshan AT menglongliu invitroantibacterialactivityandcombineddrugsensitivityof18lichenspeciesfromcangshan AT haiyanding invitroantibacterialactivityandcombineddrugsensitivityof18lichenspeciesfromcangshan |