CCL4L2 is a potential biomarker for differentiating central and peripheral vertigo
BackgroundCentral vertigo and peripheral vertigo are common clinical conditions with different underlying pathophysiologies. The identification of reliable biomarkers for differential diagnosis remains a challenge.ObjectivesThis study aimed to explore the differential expression of CCL4L2 in the ser...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Integrative Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnint.2025.1620845/full |
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| author | Xia Hong Yuan Li Chenjuan Tao Gaofeng Wang |
| author_facet | Xia Hong Yuan Li Chenjuan Tao Gaofeng Wang |
| author_sort | Xia Hong |
| collection | DOAJ |
| description | BackgroundCentral vertigo and peripheral vertigo are common clinical conditions with different underlying pathophysiologies. The identification of reliable biomarkers for differential diagnosis remains a challenge.ObjectivesThis study aimed to explore the differential expression of CCL4L2 in the serum of patients with central and peripheral vertigo and assess its diagnostic potential.MethodsA total of 180 patients (90 central vertigo, 90 peripheral vertigo) were enrolled. RNA sequencing was on serum samples to identify differentially expressed genes (DEGs). Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis revealed relevant biological pathways. The expression of CCL4L2 was measured using RT-qPCR, and its diagnostic performance was evaluated by Receiver operating characteristic (ROC) curve analysis. The correlation between CCL4L2 expression and biomarkers NSE and S100β was also assessed.ResultsRNA sequencing revealed significant differences in gene expression between central vertigo and peripheral vertigo groups. The KEGG pathway analysis identified several enriched pathways, including NF-κB signaling, where CCL4L2 was a key gene. CCL4L2 expression was significantly higher in the CV group compared to the PV group (p < 0.001). ROC analysis demonstrated high diagnostic accuracy for CCL4L2 in distinguishing CV from PV (AUC = 0.909, p < 0.001). Additionally, moderate positive correlations were observed between CCL4L2 and NSE (r = 0.475, p < 0.001), and a weaker correlation with S100β (r = 0.364, p < 0.001).ConclusionCCL4L2 may serve as a potential biomarker for differentiating central from peripheral vertigo. Its expression is closely associated with inflammatory pathways, making it a promising target for further investigation in vertigo diagnostics. |
| format | Article |
| id | doaj-art-3bc99efb8cf04d5bbf3231ba3e3937d1 |
| institution | Kabale University |
| issn | 1662-5145 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Integrative Neuroscience |
| spelling | doaj-art-3bc99efb8cf04d5bbf3231ba3e3937d12025-08-20T03:28:09ZengFrontiers Media S.A.Frontiers in Integrative Neuroscience1662-51452025-07-011910.3389/fnint.2025.16208451620845CCL4L2 is a potential biomarker for differentiating central and peripheral vertigoXia Hong0Yuan Li1Chenjuan Tao2Gaofeng Wang3Internal Department One, The Fourth People’s Hospital of Lin‘an District, Hangzhou, Zhejiang, ChinaDepartment of Otolaryngology, Affiliated Hospital of Hangzhou Normal University (Hangzhou Second People’s Hospital), Hangzhou, Zhejiang, ChinaDepartment of Neurology, Affiliated Hospital of Hangzhou Normal University (Hangzhou Second People’s Hospital), Hangzhou, Zhejiang, ChinaDepartment of Otorhinolaryngology, The Fourth People’s Hospital of Lin’an District, Hangzhou, Zhejiang, ChinaBackgroundCentral vertigo and peripheral vertigo are common clinical conditions with different underlying pathophysiologies. The identification of reliable biomarkers for differential diagnosis remains a challenge.ObjectivesThis study aimed to explore the differential expression of CCL4L2 in the serum of patients with central and peripheral vertigo and assess its diagnostic potential.MethodsA total of 180 patients (90 central vertigo, 90 peripheral vertigo) were enrolled. RNA sequencing was on serum samples to identify differentially expressed genes (DEGs). Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis revealed relevant biological pathways. The expression of CCL4L2 was measured using RT-qPCR, and its diagnostic performance was evaluated by Receiver operating characteristic (ROC) curve analysis. The correlation between CCL4L2 expression and biomarkers NSE and S100β was also assessed.ResultsRNA sequencing revealed significant differences in gene expression between central vertigo and peripheral vertigo groups. The KEGG pathway analysis identified several enriched pathways, including NF-κB signaling, where CCL4L2 was a key gene. CCL4L2 expression was significantly higher in the CV group compared to the PV group (p < 0.001). ROC analysis demonstrated high diagnostic accuracy for CCL4L2 in distinguishing CV from PV (AUC = 0.909, p < 0.001). Additionally, moderate positive correlations were observed between CCL4L2 and NSE (r = 0.475, p < 0.001), and a weaker correlation with S100β (r = 0.364, p < 0.001).ConclusionCCL4L2 may serve as a potential biomarker for differentiating central from peripheral vertigo. Its expression is closely associated with inflammatory pathways, making it a promising target for further investigation in vertigo diagnostics.https://www.frontiersin.org/articles/10.3389/fnint.2025.1620845/fullcentral vertigoperipheral vertigoCCL4L2biomarkerROC curve analysis |
| spellingShingle | Xia Hong Yuan Li Chenjuan Tao Gaofeng Wang CCL4L2 is a potential biomarker for differentiating central and peripheral vertigo Frontiers in Integrative Neuroscience central vertigo peripheral vertigo CCL4L2 biomarker ROC curve analysis |
| title | CCL4L2 is a potential biomarker for differentiating central and peripheral vertigo |
| title_full | CCL4L2 is a potential biomarker for differentiating central and peripheral vertigo |
| title_fullStr | CCL4L2 is a potential biomarker for differentiating central and peripheral vertigo |
| title_full_unstemmed | CCL4L2 is a potential biomarker for differentiating central and peripheral vertigo |
| title_short | CCL4L2 is a potential biomarker for differentiating central and peripheral vertigo |
| title_sort | ccl4l2 is a potential biomarker for differentiating central and peripheral vertigo |
| topic | central vertigo peripheral vertigo CCL4L2 biomarker ROC curve analysis |
| url | https://www.frontiersin.org/articles/10.3389/fnint.2025.1620845/full |
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