A summer in the greater Paris: trophic status of peri-urban lakes shapes prokaryotic community structure and functional potential

Abstract With more than 12 million inhabitants, the Greater Paris offers a “natural laboratory” to explore the effects of eutrophication on freshwater lake’s microbiomes within a relative restricted area (~ 70 km radius). Here, a 4-months survey was carried out during summertime to monitor planktoni...

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Main Authors: Pierre Foucault, Sébastien Halary, Charlotte Duval, Midoli Goto, Benjamin Marie, Sahima Hamlaoui, Ludwig Jardillier, Dominique Lamy, Emilie Lance, Emmanuelle Raimbault, Fayçal Allouti, Marc Troussellier, Cécile Bernard, Julie Leloup, Sébastien Duperron
Format: Article
Language:English
Published: BMC 2025-02-01
Series:Environmental Microbiome
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Online Access:https://doi.org/10.1186/s40793-025-00681-x
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author Pierre Foucault
Sébastien Halary
Charlotte Duval
Midoli Goto
Benjamin Marie
Sahima Hamlaoui
Ludwig Jardillier
Dominique Lamy
Emilie Lance
Emmanuelle Raimbault
Fayçal Allouti
Marc Troussellier
Cécile Bernard
Julie Leloup
Sébastien Duperron
author_facet Pierre Foucault
Sébastien Halary
Charlotte Duval
Midoli Goto
Benjamin Marie
Sahima Hamlaoui
Ludwig Jardillier
Dominique Lamy
Emilie Lance
Emmanuelle Raimbault
Fayçal Allouti
Marc Troussellier
Cécile Bernard
Julie Leloup
Sébastien Duperron
author_sort Pierre Foucault
collection DOAJ
description Abstract With more than 12 million inhabitants, the Greater Paris offers a “natural laboratory” to explore the effects of eutrophication on freshwater lake’s microbiomes within a relative restricted area (~ 70 km radius). Here, a 4-months survey was carried out during summertime to monitor planktonic microbial communities of nine lakes located around Paris (Île-de-France, France) of comparable morphologies, yet distinct trophic statuses from mesotrophic to hypereutrophic. By thus minimizing the confounding factors, we investigated how trophic status could influence prokaryotic community structures (16S rRNA gene sequencing) and functions (shotgun metagenomics). These freshwater lakes harbored highly distinct and diverse prokaryotic communities, and their trophic status appears as the main driver explaining both differences in community structure and functional potential. Although their gene pool was quite stable and shared among lakes, taxonomical and functional changes were correlated. According to trophic status, differences in phosphorus metabolism-related genes were highlighted among the relevant functions involved in the biogeochemical cycles. Overall, hypereutrophic lakes microbiomes displayed the highest contrast and heterogeneity over time, suggesting a specific microbial regime shift compared to eutrophic and mesotrophic lakes.
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spelling doaj-art-3bc3fbc750e249c2b021e4408a925df22025-08-20T01:57:27ZengBMCEnvironmental Microbiome2524-63722025-02-0120111610.1186/s40793-025-00681-xA summer in the greater Paris: trophic status of peri-urban lakes shapes prokaryotic community structure and functional potentialPierre Foucault0Sébastien Halary1Charlotte Duval2Midoli Goto3Benjamin Marie4Sahima Hamlaoui5Ludwig Jardillier6Dominique Lamy7Emilie Lance8Emmanuelle Raimbault9Fayçal Allouti10Marc Troussellier11Cécile Bernard12Julie Leloup13Sébastien Duperron14Muséum National d’Histoire Naturelle, UMR 7245 CNRS-MNHN, Molécules de Communication et Adaptation des Microorganismes (MCAM)Muséum National d’Histoire Naturelle, UMR 7245 CNRS-MNHN, Molécules de Communication et Adaptation des Microorganismes (MCAM)Muséum National d’Histoire Naturelle, UMR 7245 CNRS-MNHN, Molécules de Communication et Adaptation des Microorganismes (MCAM)Muséum National d’Histoire Naturelle, UMR 7245 CNRS-MNHN, Molécules de Communication et Adaptation des Microorganismes (MCAM)Muséum National d’Histoire Naturelle, UMR 7245 CNRS-MNHN, Molécules de Communication et Adaptation des Microorganismes (MCAM)Muséum National d’Histoire Naturelle, UMR 7245 CNRS-MNHN, Molécules de Communication et Adaptation des Microorganismes (MCAM)Université Paris-Saclay, UMR 8079 Univ. Paris-Saclay-CNRS-AgroParisTech, Unité d’Écologie Systématique et Évolution (ESE)Institut d’Écologie et des Sciences de l’Environnement de Paris (iEES-Paris), Sorbonne Université, UMR 7618 CNRS-INRA-IRD-Univ. Paris Cité-UPECMuséum National d’Histoire Naturelle, UMR 7245 CNRS-MNHN, Molécules de Communication et Adaptation des Microorganismes (MCAM)Institut de Physique du Globe de Paris, UMR 7154, Univ. Paris Cité-CNRSMuséum National d’Histoire Naturelle, UAR 7200 MNHN, Acquisition et Analyses de Données pour l’Histoire naturelle (2AD)Marine Biodiversity, Exploitation & Conservation (MARBEC), Univ. Montpellier-CNRS- Ifremer-IRDMuséum National d’Histoire Naturelle, UMR 7245 CNRS-MNHN, Molécules de Communication et Adaptation des Microorganismes (MCAM)Institut d’Écologie et des Sciences de l’Environnement de Paris (iEES-Paris), Sorbonne Université, UMR 7618 CNRS-INRA-IRD-Univ. Paris Cité-UPECMuséum National d’Histoire Naturelle, UMR 7245 CNRS-MNHN, Molécules de Communication et Adaptation des Microorganismes (MCAM)Abstract With more than 12 million inhabitants, the Greater Paris offers a “natural laboratory” to explore the effects of eutrophication on freshwater lake’s microbiomes within a relative restricted area (~ 70 km radius). Here, a 4-months survey was carried out during summertime to monitor planktonic microbial communities of nine lakes located around Paris (Île-de-France, France) of comparable morphologies, yet distinct trophic statuses from mesotrophic to hypereutrophic. By thus minimizing the confounding factors, we investigated how trophic status could influence prokaryotic community structures (16S rRNA gene sequencing) and functions (shotgun metagenomics). These freshwater lakes harbored highly distinct and diverse prokaryotic communities, and their trophic status appears as the main driver explaining both differences in community structure and functional potential. Although their gene pool was quite stable and shared among lakes, taxonomical and functional changes were correlated. According to trophic status, differences in phosphorus metabolism-related genes were highlighted among the relevant functions involved in the biogeochemical cycles. Overall, hypereutrophic lakes microbiomes displayed the highest contrast and heterogeneity over time, suggesting a specific microbial regime shift compared to eutrophic and mesotrophic lakes.https://doi.org/10.1186/s40793-025-00681-xEutrophicationMicrobiomeMetagenomeFreshwater ecologyPhytoplankton
spellingShingle Pierre Foucault
Sébastien Halary
Charlotte Duval
Midoli Goto
Benjamin Marie
Sahima Hamlaoui
Ludwig Jardillier
Dominique Lamy
Emilie Lance
Emmanuelle Raimbault
Fayçal Allouti
Marc Troussellier
Cécile Bernard
Julie Leloup
Sébastien Duperron
A summer in the greater Paris: trophic status of peri-urban lakes shapes prokaryotic community structure and functional potential
Environmental Microbiome
Eutrophication
Microbiome
Metagenome
Freshwater ecology
Phytoplankton
title A summer in the greater Paris: trophic status of peri-urban lakes shapes prokaryotic community structure and functional potential
title_full A summer in the greater Paris: trophic status of peri-urban lakes shapes prokaryotic community structure and functional potential
title_fullStr A summer in the greater Paris: trophic status of peri-urban lakes shapes prokaryotic community structure and functional potential
title_full_unstemmed A summer in the greater Paris: trophic status of peri-urban lakes shapes prokaryotic community structure and functional potential
title_short A summer in the greater Paris: trophic status of peri-urban lakes shapes prokaryotic community structure and functional potential
title_sort summer in the greater paris trophic status of peri urban lakes shapes prokaryotic community structure and functional potential
topic Eutrophication
Microbiome
Metagenome
Freshwater ecology
Phytoplankton
url https://doi.org/10.1186/s40793-025-00681-x
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