Using QCM‐D for Real‐Time Analysis of Cell Adhesion Dynamics at Biointerfaces
Abstract Efficient cell adhesion is crucial for host tissue attachment and improving implant integration. This study aims at investigating adhesion of human gingival fibroblasts to functionalized biomaterial surfaces using quartz crystal microbalance with dissipation (QCM‐D) and analyzing the dynami...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-05-01
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| Series: | Advanced Materials Interfaces |
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| Online Access: | https://doi.org/10.1002/admi.202400811 |
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| author | Agnes Rogala Hanna Tiainen Daria Zaytseva‐Zotova |
| author_facet | Agnes Rogala Hanna Tiainen Daria Zaytseva‐Zotova |
| author_sort | Agnes Rogala |
| collection | DOAJ |
| description | Abstract Efficient cell adhesion is crucial for host tissue attachment and improving implant integration. This study aims at investigating adhesion of human gingival fibroblasts to functionalized biomaterial surfaces using quartz crystal microbalance with dissipation (QCM‐D) and analyzing the dynamics of focal adhesion (FA) formation during this process. The QCM‐D responses are correlated with changes in cell morphology and activation of focal adhesion kinase (FAK) in the absence or presence of serum proteins. As model surfaces, this work uses polystyrene oxidized by UV/ozone treatment (PSox) and tannic acid nanocoating formed on polystyrene (TA60). Compared to PSox, reduced cell adhesion on TA60 with fibroblast establishing fewer FAs is observed in this work. The addition of serum promotes more stable cell adhesion, although the adsorbed protein corona slows initial cell spreading and FAK activation. Further, this work examines whether there is a relationship between QCM‐D responses and FAK activation. The data shows a positive correlation between the energy dissipation and temporal changes in phosphorylated FAK (pY397FAK), although the absolute value of pY397FAK cannot be derived from the QCM‐D response. Thus, this study demonstrates the usefulness of the QCM‐D as a technique for quantitative non‐invasive analysis of cell adhesion to functionalized surfaces. |
| format | Article |
| id | doaj-art-3bc0686ea6be4dcd84379ebcfd65c7a1 |
| institution | OA Journals |
| issn | 2196-7350 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj-art-3bc0686ea6be4dcd84379ebcfd65c7a12025-08-20T01:48:16ZengWiley-VCHAdvanced Materials Interfaces2196-73502025-05-01129n/an/a10.1002/admi.202400811Using QCM‐D for Real‐Time Analysis of Cell Adhesion Dynamics at BiointerfacesAgnes Rogala0Hanna Tiainen1Daria Zaytseva‐Zotova2Department of Biomaterials Institute of Clinical Dentistry University of Oslo Postboks 1109 Blindern Oslo 0317 NorwayDepartment of Biomaterials Institute of Clinical Dentistry University of Oslo Postboks 1109 Blindern Oslo 0317 NorwayDepartment of Biomaterials Institute of Clinical Dentistry University of Oslo Postboks 1109 Blindern Oslo 0317 NorwayAbstract Efficient cell adhesion is crucial for host tissue attachment and improving implant integration. This study aims at investigating adhesion of human gingival fibroblasts to functionalized biomaterial surfaces using quartz crystal microbalance with dissipation (QCM‐D) and analyzing the dynamics of focal adhesion (FA) formation during this process. The QCM‐D responses are correlated with changes in cell morphology and activation of focal adhesion kinase (FAK) in the absence or presence of serum proteins. As model surfaces, this work uses polystyrene oxidized by UV/ozone treatment (PSox) and tannic acid nanocoating formed on polystyrene (TA60). Compared to PSox, reduced cell adhesion on TA60 with fibroblast establishing fewer FAs is observed in this work. The addition of serum promotes more stable cell adhesion, although the adsorbed protein corona slows initial cell spreading and FAK activation. Further, this work examines whether there is a relationship between QCM‐D responses and FAK activation. The data shows a positive correlation between the energy dissipation and temporal changes in phosphorylated FAK (pY397FAK), although the absolute value of pY397FAK cannot be derived from the QCM‐D response. Thus, this study demonstrates the usefulness of the QCM‐D as a technique for quantitative non‐invasive analysis of cell adhesion to functionalized surfaces.https://doi.org/10.1002/admi.202400811FAKfocal adhesionsQCM‐Dsurface functionalizationtannic acid |
| spellingShingle | Agnes Rogala Hanna Tiainen Daria Zaytseva‐Zotova Using QCM‐D for Real‐Time Analysis of Cell Adhesion Dynamics at Biointerfaces Advanced Materials Interfaces FAK focal adhesions QCM‐D surface functionalization tannic acid |
| title | Using QCM‐D for Real‐Time Analysis of Cell Adhesion Dynamics at Biointerfaces |
| title_full | Using QCM‐D for Real‐Time Analysis of Cell Adhesion Dynamics at Biointerfaces |
| title_fullStr | Using QCM‐D for Real‐Time Analysis of Cell Adhesion Dynamics at Biointerfaces |
| title_full_unstemmed | Using QCM‐D for Real‐Time Analysis of Cell Adhesion Dynamics at Biointerfaces |
| title_short | Using QCM‐D for Real‐Time Analysis of Cell Adhesion Dynamics at Biointerfaces |
| title_sort | using qcm d for real time analysis of cell adhesion dynamics at biointerfaces |
| topic | FAK focal adhesions QCM‐D surface functionalization tannic acid |
| url | https://doi.org/10.1002/admi.202400811 |
| work_keys_str_mv | AT agnesrogala usingqcmdforrealtimeanalysisofcelladhesiondynamicsatbiointerfaces AT hannatiainen usingqcmdforrealtimeanalysisofcelladhesiondynamicsatbiointerfaces AT dariazaytsevazotova usingqcmdforrealtimeanalysisofcelladhesiondynamicsatbiointerfaces |