Identification of Wheat Inflorescence Development-Related Genes Using a Comparative Transcriptomics Approach
Inflorescence represents the highly specialized plant tissue producing the grains. Although key genes regulating flower initiation and development are conserved, the mechanism regulating fertility is still not well explained. To identify genes and gene network underlying inflorescence morphology and...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2018/6897032 |
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| author | Lingjie Ma Sheng-Wei Ma Qingyan Deng Yang Yuan Zhaoyan Wei Haiyan Jia Zhengqiang Ma |
| author_facet | Lingjie Ma Sheng-Wei Ma Qingyan Deng Yang Yuan Zhaoyan Wei Haiyan Jia Zhengqiang Ma |
| author_sort | Lingjie Ma |
| collection | DOAJ |
| description | Inflorescence represents the highly specialized plant tissue producing the grains. Although key genes regulating flower initiation and development are conserved, the mechanism regulating fertility is still not well explained. To identify genes and gene network underlying inflorescence morphology and fertility of bread wheat, expressed sequence tags (ESTs) from different tissues were analyzed using a comparative transcriptomics approach. Based on statistical comparison of EST frequencies of individual genes in EST pools representing different tissues and verification with RT-PCR and RNA-seq data, 170 genes of 59 gene sets predominantly expressed in the inflorescence were obtained. Nearly one-third of the gene sets displayed differentiated expression profiles in terms of their subgenome orthologs. The identified genes, most of which were predominantly expressed in anthers, encode proteins involved in wheat floral identity determination, anther and pollen development, pollen-pistil interaction, and others. Particularly, 25 annotated gene sets are associated with pollen wall formation, of which 18 encode enzymes or proteins participating in lipid metabolic pathway, including fatty acid ω-hydroxylation, alkane and fatty alcohol biosynthesis, and glycerophospholipid metabolism. We showed that the comparative transcriptomics approach was effective in identifying genes for reproductive development and found that lipid metabolism was particularly active in wheat anthers. |
| format | Article |
| id | doaj-art-3bbc859095e1488eb70a54ef0b97ef0a |
| institution | OA Journals |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-3bbc859095e1488eb70a54ef0b97ef0a2025-08-20T02:08:00ZengWileyInternational Journal of Genomics2314-436X2314-43782018-01-01201810.1155/2018/68970326897032Identification of Wheat Inflorescence Development-Related Genes Using a Comparative Transcriptomics ApproachLingjie Ma0Sheng-Wei Ma1Qingyan Deng2Yang Yuan3Zhaoyan Wei4Haiyan Jia5Zhengqiang Ma6The Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Jiangsu 210095, ChinaThe Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Jiangsu 210095, ChinaThe Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Jiangsu 210095, ChinaThe Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Jiangsu 210095, ChinaThe Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Jiangsu 210095, ChinaThe Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Jiangsu 210095, ChinaThe Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Jiangsu 210095, ChinaInflorescence represents the highly specialized plant tissue producing the grains. Although key genes regulating flower initiation and development are conserved, the mechanism regulating fertility is still not well explained. To identify genes and gene network underlying inflorescence morphology and fertility of bread wheat, expressed sequence tags (ESTs) from different tissues were analyzed using a comparative transcriptomics approach. Based on statistical comparison of EST frequencies of individual genes in EST pools representing different tissues and verification with RT-PCR and RNA-seq data, 170 genes of 59 gene sets predominantly expressed in the inflorescence were obtained. Nearly one-third of the gene sets displayed differentiated expression profiles in terms of their subgenome orthologs. The identified genes, most of which were predominantly expressed in anthers, encode proteins involved in wheat floral identity determination, anther and pollen development, pollen-pistil interaction, and others. Particularly, 25 annotated gene sets are associated with pollen wall formation, of which 18 encode enzymes or proteins participating in lipid metabolic pathway, including fatty acid ω-hydroxylation, alkane and fatty alcohol biosynthesis, and glycerophospholipid metabolism. We showed that the comparative transcriptomics approach was effective in identifying genes for reproductive development and found that lipid metabolism was particularly active in wheat anthers.http://dx.doi.org/10.1155/2018/6897032 |
| spellingShingle | Lingjie Ma Sheng-Wei Ma Qingyan Deng Yang Yuan Zhaoyan Wei Haiyan Jia Zhengqiang Ma Identification of Wheat Inflorescence Development-Related Genes Using a Comparative Transcriptomics Approach International Journal of Genomics |
| title | Identification of Wheat Inflorescence Development-Related Genes Using a Comparative Transcriptomics Approach |
| title_full | Identification of Wheat Inflorescence Development-Related Genes Using a Comparative Transcriptomics Approach |
| title_fullStr | Identification of Wheat Inflorescence Development-Related Genes Using a Comparative Transcriptomics Approach |
| title_full_unstemmed | Identification of Wheat Inflorescence Development-Related Genes Using a Comparative Transcriptomics Approach |
| title_short | Identification of Wheat Inflorescence Development-Related Genes Using a Comparative Transcriptomics Approach |
| title_sort | identification of wheat inflorescence development related genes using a comparative transcriptomics approach |
| url | http://dx.doi.org/10.1155/2018/6897032 |
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