A functionally significant polymorphism in ID3 is associated with human coronary pathology.

<h4>Aims</h4>We previously identified association between the ID3 SNP rs11574 and carotid intima-media thickness in the Diabetes Heart Study, a predominantly White diabetic population. The nonsynonymous SNP rs11574 results in an amino acid substitution in the C-terminal region of ID3, at...

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Main Authors: Ani Manichaikul, Stephen S Rich, Heather Perry, Joseph Yeboah, Michelle Law, Molly Davis, Matthew Parker, Michael Ragosta, Jessica J Connelly, Coleen A McNamara, Angela M Taylor
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090222&type=printable
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author Ani Manichaikul
Stephen S Rich
Heather Perry
Joseph Yeboah
Michelle Law
Molly Davis
Matthew Parker
Michael Ragosta
Jessica J Connelly
Coleen A McNamara
Angela M Taylor
author_facet Ani Manichaikul
Stephen S Rich
Heather Perry
Joseph Yeboah
Michelle Law
Molly Davis
Matthew Parker
Michael Ragosta
Jessica J Connelly
Coleen A McNamara
Angela M Taylor
author_sort Ani Manichaikul
collection DOAJ
description <h4>Aims</h4>We previously identified association between the ID3 SNP rs11574 and carotid intima-media thickness in the Diabetes Heart Study, a predominantly White diabetic population. The nonsynonymous SNP rs11574 results in an amino acid substitution in the C-terminal region of ID3, attenuating the dominant negative function of ID3 as an inhibitor of basic HLH factor E12-mediated transcription. In the current investigation, we characterize the association between the functionally significant polymorphism in ID3, rs11574, with human coronary pathology.<h4>Methods and results</h4>The Multi-Ethnic Study of Atherosclerosis (MESA) is a longitudinal study of subclinical cardiovascular disease, including non-Hispanic White (n = 2,588), African American (n = 2,560) and Hispanic (n = 2,130) participants with data on coronary artery calcium (CAC). The Coronary Assessment in Virginia cohort (CAVA) included 71 patients aged 30-80 years, undergoing a medically necessary cardiac catheterization and intravascular ultrasound (IVUS) at the University of Virginia. ID3 SNP rs11574 risk allele was associated with the presence of CAC in MESA Whites (P = 0.017). In addition, the risk allele was associated with greater atheroma burden and stenosis in the CAVA cohort (P = 0.003, P = 0.04 respectively). The risk allele remained predictive of atheroma burden in multivariate analysis (Model 1: covariates age, gender, and LDL, regression coefficient = 9.578, SE = 3.657, p = 0.0110; Model 2: covariates Model 1, presence of hypertension, presence of diabetes, regression coefficient = 8.389, SE = 4.788, p = 0.0163).<h4>Conclusions</h4>We present additional cohorts that demonstrate association of ID3 SNP rs11574 directly with human coronary artery pathology as measured by CAC and IVUS: one a multiethnic, relatively healthy population with low levels of diabetes and the second a predominantly White population with a higher incidence of T2DM referred for cardiac catheterization.
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spelling doaj-art-3bae6df22156477eb4d7bf8f07e8f80b2025-08-20T03:46:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e9022210.1371/journal.pone.0090222A functionally significant polymorphism in ID3 is associated with human coronary pathology.Ani ManichaikulStephen S RichHeather PerryJoseph YeboahMichelle LawMolly DavisMatthew ParkerMichael RagostaJessica J ConnellyColeen A McNamaraAngela M Taylor<h4>Aims</h4>We previously identified association between the ID3 SNP rs11574 and carotid intima-media thickness in the Diabetes Heart Study, a predominantly White diabetic population. The nonsynonymous SNP rs11574 results in an amino acid substitution in the C-terminal region of ID3, attenuating the dominant negative function of ID3 as an inhibitor of basic HLH factor E12-mediated transcription. In the current investigation, we characterize the association between the functionally significant polymorphism in ID3, rs11574, with human coronary pathology.<h4>Methods and results</h4>The Multi-Ethnic Study of Atherosclerosis (MESA) is a longitudinal study of subclinical cardiovascular disease, including non-Hispanic White (n = 2,588), African American (n = 2,560) and Hispanic (n = 2,130) participants with data on coronary artery calcium (CAC). The Coronary Assessment in Virginia cohort (CAVA) included 71 patients aged 30-80 years, undergoing a medically necessary cardiac catheterization and intravascular ultrasound (IVUS) at the University of Virginia. ID3 SNP rs11574 risk allele was associated with the presence of CAC in MESA Whites (P = 0.017). In addition, the risk allele was associated with greater atheroma burden and stenosis in the CAVA cohort (P = 0.003, P = 0.04 respectively). The risk allele remained predictive of atheroma burden in multivariate analysis (Model 1: covariates age, gender, and LDL, regression coefficient = 9.578, SE = 3.657, p = 0.0110; Model 2: covariates Model 1, presence of hypertension, presence of diabetes, regression coefficient = 8.389, SE = 4.788, p = 0.0163).<h4>Conclusions</h4>We present additional cohorts that demonstrate association of ID3 SNP rs11574 directly with human coronary artery pathology as measured by CAC and IVUS: one a multiethnic, relatively healthy population with low levels of diabetes and the second a predominantly White population with a higher incidence of T2DM referred for cardiac catheterization.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090222&type=printable
spellingShingle Ani Manichaikul
Stephen S Rich
Heather Perry
Joseph Yeboah
Michelle Law
Molly Davis
Matthew Parker
Michael Ragosta
Jessica J Connelly
Coleen A McNamara
Angela M Taylor
A functionally significant polymorphism in ID3 is associated with human coronary pathology.
PLoS ONE
title A functionally significant polymorphism in ID3 is associated with human coronary pathology.
title_full A functionally significant polymorphism in ID3 is associated with human coronary pathology.
title_fullStr A functionally significant polymorphism in ID3 is associated with human coronary pathology.
title_full_unstemmed A functionally significant polymorphism in ID3 is associated with human coronary pathology.
title_short A functionally significant polymorphism in ID3 is associated with human coronary pathology.
title_sort functionally significant polymorphism in id3 is associated with human coronary pathology
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0090222&type=printable
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