Detection and genotyping of Leptospira spp. from the kidneys of a seemingly healthy pig slaughtered for human consumption
Introduction: Leptospirosis is a zoonotic disease caused by pathogenic Leptospira spp. Leptospirosis is maintained in an environment due to chronic kidney infection of a wide variety of domestic, peridomestic and wild reservoir mammals. In this study the role of pigs in maintenance of leptospires o...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2015-05-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/5727 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850036364026839040 |
|---|---|
| author | Ashutosh Verma Esteban Soto Oscar Illanes Souvik Ghosh Carmen Fuentealba |
| author_facet | Ashutosh Verma Esteban Soto Oscar Illanes Souvik Ghosh Carmen Fuentealba |
| author_sort | Ashutosh Verma |
| collection | DOAJ |
| description | Introduction: Leptospirosis is a zoonotic disease caused by pathogenic Leptospira spp. Leptospirosis is maintained in an environment due to chronic kidney infection of a wide variety of domestic, peridomestic and wild reservoir mammals. In this study the role of pigs in maintenance of leptospires on the Caribbean island of St. Kitts was investigated.
Methodology: The condemned kidneys of 60 pigs slaughtered at a St. Kitts abattoir were screened by a quantitative-PCR for the presence of Leptospira spp. Positive samples were genotyped using a six-gene based multilocus sequence typing scheme.
Results: Leptospiral DNA was detected in the kidneys of one of the 60 pigs. Multilocus sequence typing identified the infecting species to be L. interrogans.
Conclusions: Detection of this zoonotic pathogen in the kidneys of a seemingly healthy pig raises concerns regarding the subclinical carriers of the disease among the island’s swine population.
|
| format | Article |
| id | doaj-art-3bab1e685ec2402bbafec0e37f83865d |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-05-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-3bab1e685ec2402bbafec0e37f83865d2025-08-20T02:57:12ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-05-0190510.3855/jidc.5727Detection and genotyping of Leptospira spp. from the kidneys of a seemingly healthy pig slaughtered for human consumptionAshutosh Verma0Esteban Soto1Oscar Illanes2Souvik Ghosh3Carmen Fuentealba4Ross University School of Veterinary Medicine, Basseterre, St. KittsRoss University School of Veterinary Medicine, Basseterre, St. KittsRoss University School of Veterinary Medicine, Basseterre, St. KittsRoss University School of Veterinary Medicine, Basseterre, St. KittsRoss University School of Veterinary Medicine, Basseterre, St. KittsIntroduction: Leptospirosis is a zoonotic disease caused by pathogenic Leptospira spp. Leptospirosis is maintained in an environment due to chronic kidney infection of a wide variety of domestic, peridomestic and wild reservoir mammals. In this study the role of pigs in maintenance of leptospires on the Caribbean island of St. Kitts was investigated. Methodology: The condemned kidneys of 60 pigs slaughtered at a St. Kitts abattoir were screened by a quantitative-PCR for the presence of Leptospira spp. Positive samples were genotyped using a six-gene based multilocus sequence typing scheme. Results: Leptospiral DNA was detected in the kidneys of one of the 60 pigs. Multilocus sequence typing identified the infecting species to be L. interrogans. Conclusions: Detection of this zoonotic pathogen in the kidneys of a seemingly healthy pig raises concerns regarding the subclinical carriers of the disease among the island’s swine population. https://jidc.org/index.php/journal/article/view/5727LeptospirosisLeptospiraswinegenotypingMulti-locus sequence typing (MLST) |
| spellingShingle | Ashutosh Verma Esteban Soto Oscar Illanes Souvik Ghosh Carmen Fuentealba Detection and genotyping of Leptospira spp. from the kidneys of a seemingly healthy pig slaughtered for human consumption Journal of Infection in Developing Countries Leptospirosis Leptospira swine genotyping Multi-locus sequence typing (MLST) |
| title | Detection and genotyping of Leptospira spp. from the kidneys of a seemingly healthy pig slaughtered for human consumption |
| title_full | Detection and genotyping of Leptospira spp. from the kidneys of a seemingly healthy pig slaughtered for human consumption |
| title_fullStr | Detection and genotyping of Leptospira spp. from the kidneys of a seemingly healthy pig slaughtered for human consumption |
| title_full_unstemmed | Detection and genotyping of Leptospira spp. from the kidneys of a seemingly healthy pig slaughtered for human consumption |
| title_short | Detection and genotyping of Leptospira spp. from the kidneys of a seemingly healthy pig slaughtered for human consumption |
| title_sort | detection and genotyping of leptospira spp from the kidneys of a seemingly healthy pig slaughtered for human consumption |
| topic | Leptospirosis Leptospira swine genotyping Multi-locus sequence typing (MLST) |
| url | https://jidc.org/index.php/journal/article/view/5727 |
| work_keys_str_mv | AT ashutoshverma detectionandgenotypingofleptospirasppfromthekidneysofaseeminglyhealthypigslaughteredforhumanconsumption AT estebansoto detectionandgenotypingofleptospirasppfromthekidneysofaseeminglyhealthypigslaughteredforhumanconsumption AT oscarillanes detectionandgenotypingofleptospirasppfromthekidneysofaseeminglyhealthypigslaughteredforhumanconsumption AT souvikghosh detectionandgenotypingofleptospirasppfromthekidneysofaseeminglyhealthypigslaughteredforhumanconsumption AT carmenfuentealba detectionandgenotypingofleptospirasppfromthekidneysofaseeminglyhealthypigslaughteredforhumanconsumption |