Comparative Transcriptome Analysis of Eggplant (<i>Solanum melongena</i> L.) Peels with Different Glossiness

Peel glossiness is an important commercial trait of eggplant (<i>Solanum melongena</i> L.). In this study, two eggplant-inbred lines with different levels of peel glossiness were used to identify genes related to peel glossiness. Paraffin section analysis showed that increased wax thickn...

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Main Authors: Hong Wang, Zhixing Nie, Tonglin Wang, Shuhuan Yang, Jirong Zheng
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/14/12/3063
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author Hong Wang
Zhixing Nie
Tonglin Wang
Shuhuan Yang
Jirong Zheng
author_facet Hong Wang
Zhixing Nie
Tonglin Wang
Shuhuan Yang
Jirong Zheng
author_sort Hong Wang
collection DOAJ
description Peel glossiness is an important commercial trait of eggplant (<i>Solanum melongena</i> L.). In this study, two eggplant-inbred lines with different levels of peel glossiness were used to identify genes related to peel glossiness. Paraffin section analysis showed that increased wax thickness and wrinkles on the wax surface of eggplant peels decreased glossiness. Differential gene expression related to eggplant peel glossiness was analyzed by comparing the transcriptomes of eggplant peels with different gloss levels and at different developmental stages. The results identified 996 differentially expressed genes (DEGs), including 502 upregulated and 494 downregulated genes, possibly related to eggplant peel glossiness. GO enrichment and KEGG enrichment analyses revealed that the DNA replication pathway (GO:0003688, GO:0006270) and the photosynthesis pathway (map00195) were downregulated and thus may be associated with reduced eggplant peel glossiness. Expression level analysis of eggplant peels with different glossiness levels revealed that a C2H2 transcription factor gene, two ERF transcription factor genes, one long-chain acyl-CoA synthetase gene, and four wax- or cutin-related genes may be associated with the glossiness of eggplant fruit peels. These findings will help guide future genetic improvements in eggplant peel glossiness.
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spelling doaj-art-3b9f3435f8a646c1b97351e847dfa82c2025-08-20T02:55:38ZengMDPI AGAgronomy2073-43952024-12-011412306310.3390/agronomy14123063Comparative Transcriptome Analysis of Eggplant (<i>Solanum melongena</i> L.) Peels with Different GlossinessHong Wang0Zhixing Nie1Tonglin Wang2Shuhuan Yang3Jirong Zheng4Vegetable Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, ChinaVegetable Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, ChinaVegetable Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, ChinaCollege of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaVegetable Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, ChinaPeel glossiness is an important commercial trait of eggplant (<i>Solanum melongena</i> L.). In this study, two eggplant-inbred lines with different levels of peel glossiness were used to identify genes related to peel glossiness. Paraffin section analysis showed that increased wax thickness and wrinkles on the wax surface of eggplant peels decreased glossiness. Differential gene expression related to eggplant peel glossiness was analyzed by comparing the transcriptomes of eggplant peels with different gloss levels and at different developmental stages. The results identified 996 differentially expressed genes (DEGs), including 502 upregulated and 494 downregulated genes, possibly related to eggplant peel glossiness. GO enrichment and KEGG enrichment analyses revealed that the DNA replication pathway (GO:0003688, GO:0006270) and the photosynthesis pathway (map00195) were downregulated and thus may be associated with reduced eggplant peel glossiness. Expression level analysis of eggplant peels with different glossiness levels revealed that a C2H2 transcription factor gene, two ERF transcription factor genes, one long-chain acyl-CoA synthetase gene, and four wax- or cutin-related genes may be associated with the glossiness of eggplant fruit peels. These findings will help guide future genetic improvements in eggplant peel glossiness.https://www.mdpi.com/2073-4395/14/12/3063eggplant (<i>Solanum</i> <i>melongena</i> L.)peel glossinessRNA-Seqwax
spellingShingle Hong Wang
Zhixing Nie
Tonglin Wang
Shuhuan Yang
Jirong Zheng
Comparative Transcriptome Analysis of Eggplant (<i>Solanum melongena</i> L.) Peels with Different Glossiness
Agronomy
eggplant (<i>Solanum</i> <i>melongena</i> L.)
peel glossiness
RNA-Seq
wax
title Comparative Transcriptome Analysis of Eggplant (<i>Solanum melongena</i> L.) Peels with Different Glossiness
title_full Comparative Transcriptome Analysis of Eggplant (<i>Solanum melongena</i> L.) Peels with Different Glossiness
title_fullStr Comparative Transcriptome Analysis of Eggplant (<i>Solanum melongena</i> L.) Peels with Different Glossiness
title_full_unstemmed Comparative Transcriptome Analysis of Eggplant (<i>Solanum melongena</i> L.) Peels with Different Glossiness
title_short Comparative Transcriptome Analysis of Eggplant (<i>Solanum melongena</i> L.) Peels with Different Glossiness
title_sort comparative transcriptome analysis of eggplant i solanum melongena i l peels with different glossiness
topic eggplant (<i>Solanum</i> <i>melongena</i> L.)
peel glossiness
RNA-Seq
wax
url https://www.mdpi.com/2073-4395/14/12/3063
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AT tonglinwang comparativetranscriptomeanalysisofeggplantisolanummelongenailpeelswithdifferentglossiness
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