Modulation of Erythrocyte Plasma Membrane Redox System Activity by Curcumin
Plasma membrane redox system (PMRS) is an electron transport chain system ubiquitously present throughout all cell types. It transfers electron from intracellular substrates to extracellular acceptors for regulation of redox status. Curcumin, isolated from Curcuma longa, has modulatory effects on ce...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2016/6025245 |
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| author | Prabhakar Singh Rajesh Kumar Kesharwani Krishna Misra Syed Ibrahim Rizvi |
| author_facet | Prabhakar Singh Rajesh Kumar Kesharwani Krishna Misra Syed Ibrahim Rizvi |
| author_sort | Prabhakar Singh |
| collection | DOAJ |
| description | Plasma membrane redox system (PMRS) is an electron transport chain system ubiquitously present throughout all cell types. It transfers electron from intracellular substrates to extracellular acceptors for regulation of redox status. Curcumin, isolated from Curcuma longa, has modulatory effects on cellular physiology due to its membrane interaction ability and antioxidant potential. The present study investigates the effect of curcumin on PMRS activity of erythrocytes isolated from Wistar rats in vitro and in vivo and validated through an in silico docking simulation study using Molegro Virtual Docker (MVD). Effects of curcumin were also evaluated on level of glutathione (GSH) and the oxidant potential of plasma measured in terms of plasma ferric equivalent oxidative potentials (PFEOP). Results show that curcumin significantly (p<0.01) downregulated the PMRS activity in a dose-dependent manner. Molecular docking results suggest that curcumin interacts with amino acids at the active site cavity of cytochrome b5 reductase, a key constituent of PMRS. Curcumin also increased the GSH level in erythrocytes and plasma while simultaneously decreasing the oxidant potential (PFEOP) of plasma. Altered PMRS activity and redox status are associated with the pathophysiology of several health complications including aging and diabetes; hence, the above finding may explain part of the role of curcumin in health beneficial effects. |
| format | Article |
| id | doaj-art-3b9e341627194e7fa2396a09f34deeed |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-3b9e341627194e7fa2396a09f34deeed2025-08-20T03:55:41ZengWileyBiochemistry Research International2090-22472090-22552016-01-01201610.1155/2016/60252456025245Modulation of Erythrocyte Plasma Membrane Redox System Activity by CurcuminPrabhakar Singh0Rajesh Kumar Kesharwani1Krishna Misra2Syed Ibrahim Rizvi3Department of Biochemistry, University of Allahabad, Allahabad 211002, IndiaDivision of Applied Science & Indo-Russian Center For Biotechnology (IRCB), Indian Institute of Information Technology, Allahabad 211012, IndiaDivision of Applied Science & Indo-Russian Center For Biotechnology (IRCB), Indian Institute of Information Technology, Allahabad 211012, IndiaDepartment of Biochemistry, University of Allahabad, Allahabad 211002, IndiaPlasma membrane redox system (PMRS) is an electron transport chain system ubiquitously present throughout all cell types. It transfers electron from intracellular substrates to extracellular acceptors for regulation of redox status. Curcumin, isolated from Curcuma longa, has modulatory effects on cellular physiology due to its membrane interaction ability and antioxidant potential. The present study investigates the effect of curcumin on PMRS activity of erythrocytes isolated from Wistar rats in vitro and in vivo and validated through an in silico docking simulation study using Molegro Virtual Docker (MVD). Effects of curcumin were also evaluated on level of glutathione (GSH) and the oxidant potential of plasma measured in terms of plasma ferric equivalent oxidative potentials (PFEOP). Results show that curcumin significantly (p<0.01) downregulated the PMRS activity in a dose-dependent manner. Molecular docking results suggest that curcumin interacts with amino acids at the active site cavity of cytochrome b5 reductase, a key constituent of PMRS. Curcumin also increased the GSH level in erythrocytes and plasma while simultaneously decreasing the oxidant potential (PFEOP) of plasma. Altered PMRS activity and redox status are associated with the pathophysiology of several health complications including aging and diabetes; hence, the above finding may explain part of the role of curcumin in health beneficial effects.http://dx.doi.org/10.1155/2016/6025245 |
| spellingShingle | Prabhakar Singh Rajesh Kumar Kesharwani Krishna Misra Syed Ibrahim Rizvi Modulation of Erythrocyte Plasma Membrane Redox System Activity by Curcumin Biochemistry Research International |
| title | Modulation of Erythrocyte Plasma Membrane Redox System Activity by Curcumin |
| title_full | Modulation of Erythrocyte Plasma Membrane Redox System Activity by Curcumin |
| title_fullStr | Modulation of Erythrocyte Plasma Membrane Redox System Activity by Curcumin |
| title_full_unstemmed | Modulation of Erythrocyte Plasma Membrane Redox System Activity by Curcumin |
| title_short | Modulation of Erythrocyte Plasma Membrane Redox System Activity by Curcumin |
| title_sort | modulation of erythrocyte plasma membrane redox system activity by curcumin |
| url | http://dx.doi.org/10.1155/2016/6025245 |
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