Genomic Analysis of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from Bloodstream Infections in South Korea
Background/Objectives: Bloodstream infection by carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) is a serious clinical problem worldwide. To study its clonal relationship and genetic features, we report the draft genome sequence of CRAB strains isolated from human blood in Sout...
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2024-11-01
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| author | Wook Jong Jeon Yoo Jung Kim Ju Hui Seo Jung Sik Yoo Dong Chan Moon |
| author_facet | Wook Jong Jeon Yoo Jung Kim Ju Hui Seo Jung Sik Yoo Dong Chan Moon |
| author_sort | Wook Jong Jeon |
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| description | Background/Objectives: Bloodstream infection by carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) is a serious clinical problem worldwide. To study its clonal relationship and genetic features, we report the draft genome sequence of CRAB strains isolated from human blood in South Korea. Methods: Among <i>A. baumannii</i> strains isolated from patients at nine general hospitals in 2020, 12 CRAB strains of different genotypes were selected. Genomic DNA was sequenced using a combination of Illumina MiSeq and Oxford Nanopore MinION platforms. Antimicrobial susceptibility testing was performed using the disk diffusion method. Antimicrobial resistance and virulence genes were investigated in silico using the Center for Genomic Epidemiology server and the Virulence Factors Database. Results: The multilocus sequence types of isolates included ST191, ST195, ST357, ST369, ST451, ST469, ST491, ST784, ST862, ST1933, ST2929, and a novel type, ST3326. The predominant sequence type, ST191, demonstrated close genetic relationships with several isolates, including ST469, ST369, ST195, ST784, ST491, and ST3326, with ST3326 classified as a subgroup of ST191. We found 18 antimicrobial resistance genes and one quaternary ammonium compound resistance gene. All examined strains harbored <i>bla</i><sub>OXA-23</sub>, which is associated with carbapenem resistance. While variations in antibiotic and disinfectant resistance genes were observed, all isolates exhibited similar virulence factors, with the exception of the biofilm and capsule production genes. Conclusions: This nationwide report of the draft genome sequence of patient-derived strains provides valuable insights into the genomic features associated with clonal relationships and antimicrobial resistance of CRAB in bloodstream infections. |
| format | Article |
| id | doaj-art-3b92afbcd2b6409a944947f98eb82c1b |
| institution | DOAJ |
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| language | English |
| publishDate | 2024-11-01 |
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| series | Antibiotics |
| spelling | doaj-art-3b92afbcd2b6409a944947f98eb82c1b2025-08-20T02:53:18ZengMDPI AGAntibiotics2079-63822024-11-011312112410.3390/antibiotics13121124Genomic Analysis of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from Bloodstream Infections in South KoreaWook Jong Jeon0Yoo Jung Kim1Ju Hui Seo2Jung Sik Yoo3Dong Chan Moon4Division of Antimicrobial Resistance Research, National Institute of Health, Korea Disease Control and Prevention Agency, 187 Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28159, Republic of KoreaDivision of Antimicrobial Resistance Research, National Institute of Health, Korea Disease Control and Prevention Agency, 187 Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28159, Republic of KoreaDivision of Antimicrobial Resistance Research, National Institute of Health, Korea Disease Control and Prevention Agency, 187 Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28159, Republic of KoreaDivision of Antimicrobial Resistance Research, National Institute of Health, Korea Disease Control and Prevention Agency, 187 Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28159, Republic of KoreaDivision of Antimicrobial Resistance Research, National Institute of Health, Korea Disease Control and Prevention Agency, 187 Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28159, Republic of KoreaBackground/Objectives: Bloodstream infection by carbapenem-resistant <i>Acinetobacter baumannii</i> (CRAB) is a serious clinical problem worldwide. To study its clonal relationship and genetic features, we report the draft genome sequence of CRAB strains isolated from human blood in South Korea. Methods: Among <i>A. baumannii</i> strains isolated from patients at nine general hospitals in 2020, 12 CRAB strains of different genotypes were selected. Genomic DNA was sequenced using a combination of Illumina MiSeq and Oxford Nanopore MinION platforms. Antimicrobial susceptibility testing was performed using the disk diffusion method. Antimicrobial resistance and virulence genes were investigated in silico using the Center for Genomic Epidemiology server and the Virulence Factors Database. Results: The multilocus sequence types of isolates included ST191, ST195, ST357, ST369, ST451, ST469, ST491, ST784, ST862, ST1933, ST2929, and a novel type, ST3326. The predominant sequence type, ST191, demonstrated close genetic relationships with several isolates, including ST469, ST369, ST195, ST784, ST491, and ST3326, with ST3326 classified as a subgroup of ST191. We found 18 antimicrobial resistance genes and one quaternary ammonium compound resistance gene. All examined strains harbored <i>bla</i><sub>OXA-23</sub>, which is associated with carbapenem resistance. While variations in antibiotic and disinfectant resistance genes were observed, all isolates exhibited similar virulence factors, with the exception of the biofilm and capsule production genes. Conclusions: This nationwide report of the draft genome sequence of patient-derived strains provides valuable insights into the genomic features associated with clonal relationships and antimicrobial resistance of CRAB in bloodstream infections.https://www.mdpi.com/2079-6382/13/12/1124<i>Acinetobacter baumannii</i>carbapenem-resistantbloodstreamgenomics |
| spellingShingle | Wook Jong Jeon Yoo Jung Kim Ju Hui Seo Jung Sik Yoo Dong Chan Moon Genomic Analysis of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from Bloodstream Infections in South Korea Antibiotics <i>Acinetobacter baumannii</i> carbapenem-resistant bloodstream genomics |
| title | Genomic Analysis of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from Bloodstream Infections in South Korea |
| title_full | Genomic Analysis of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from Bloodstream Infections in South Korea |
| title_fullStr | Genomic Analysis of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from Bloodstream Infections in South Korea |
| title_full_unstemmed | Genomic Analysis of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from Bloodstream Infections in South Korea |
| title_short | Genomic Analysis of Carbapenem-Resistant <i>Acinetobacter baumannii</i> Isolated from Bloodstream Infections in South Korea |
| title_sort | genomic analysis of carbapenem resistant i acinetobacter baumannii i isolated from bloodstream infections in south korea |
| topic | <i>Acinetobacter baumannii</i> carbapenem-resistant bloodstream genomics |
| url | https://www.mdpi.com/2079-6382/13/12/1124 |
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