Blood perfusion through ventricular assist devices induces erythrocytes to interact with leukocytes
Implantations of Ventricular Assist Devices (VADs) have significantly improved quality of life and life expectancy of end-stage heart failure patients. However, despite the advancements in the VAD designs and patient management protocols, the VAD recipients remain at risk of attaining bleeding, infe...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Biochemistry and Biophysics Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580825001724 |
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| author | Kylie M. Foster Ahmed M. El Banayosy Zheila Azartash-Namin Phillip Coghill James W. Long Edgar O'Rear Hendra Setiadi |
| author_facet | Kylie M. Foster Ahmed M. El Banayosy Zheila Azartash-Namin Phillip Coghill James W. Long Edgar O'Rear Hendra Setiadi |
| author_sort | Kylie M. Foster |
| collection | DOAJ |
| description | Implantations of Ventricular Assist Devices (VADs) have significantly improved quality of life and life expectancy of end-stage heart failure patients. However, despite the advancements in the VAD designs and patient management protocols, the VAD recipients remain at risk of attaining bleeding, infection, pump thrombosis, and stroke. Although blood trauma has been suggested as a critical factor in development of these adverse events, its consequences in inducing interactions between different types of blood cells are largely unknown.Following our recent findings on erythrocyte and leukocyte trauma in VAD recipients, we decided to explore interactions between erythrocytes and leukocytes by perfusing human whole blood through two different types of VADs, HeartMate II (HMII) and HeartMate 3 (HM3), concurrently in their respective Blood Circulatory Loop (BCL). By using a flow cytometry assay, we found increasing association between erythrocytes and leukocytes as VADs propelled blood through their BCLs. This time-dependent intercellular association was shown by increasing concomitant events of stained CD235a (specifically expressed on erythrocytes) and stained CD45 (specifically expressed on leukocytes) in the CD235a+ population. Compared to CentriMag (CM), which served as a control, VADs produced significantly higher concomitant signals. The findings described in this study have opened the need for further studies on a novel path for generation of adverse events that are commonly observed in VAD recipients, notably infection, pump thrombosis, and ischemic stroke. |
| format | Article |
| id | doaj-art-3b90b0000f1246b49110e61ecb5b4e7c |
| institution | Kabale University |
| issn | 2405-5808 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-3b90b0000f1246b49110e61ecb5b4e7c2025-08-20T03:45:44ZengElsevierBiochemistry and Biophysics Reports2405-58082025-09-014310208510.1016/j.bbrep.2025.102085Blood perfusion through ventricular assist devices induces erythrocytes to interact with leukocytesKylie M. Foster0Ahmed M. El Banayosy1Zheila Azartash-Namin2Phillip Coghill3James W. Long4Edgar O'Rear5Hendra Setiadi6School of Sustainable Chemical, Biological and Materials Engineering, Institute for Biomedical Engineering, Science and Technology, University of Oklahoma, 100 East Boyd, Sarkeys Energy Center, Norman, OK 73019, USAINTEGRIS Advanced Cardiopulmonary Care, INTEGRIS Baptist Medical Center, 3400 NW Expressway, Ste 200, Oklahoma City, OK 73112, USAVADovations Inc., 1333 Cornell Pkwy, Oklahoma City, OK 73108, USAVADovations Inc., 1333 Cornell Pkwy, Oklahoma City, OK 73108, USAINTEGRIS Advanced Cardiopulmonary Care, INTEGRIS Baptist Medical Center, 3400 NW Expressway, Ste 200, Oklahoma City, OK 73112, USASchool of Sustainable Chemical, Biological and Materials Engineering, Institute for Biomedical Engineering, Science and Technology, University of Oklahoma, 100 East Boyd, Sarkeys Energy Center, Norman, OK 73019, USAINTEGRIS Advanced Cardiopulmonary Care, INTEGRIS Baptist Medical Center, 3400 NW Expressway, Ste 200, Oklahoma City, OK 73112, USA; Corresponding author.Implantations of Ventricular Assist Devices (VADs) have significantly improved quality of life and life expectancy of end-stage heart failure patients. However, despite the advancements in the VAD designs and patient management protocols, the VAD recipients remain at risk of attaining bleeding, infection, pump thrombosis, and stroke. Although blood trauma has been suggested as a critical factor in development of these adverse events, its consequences in inducing interactions between different types of blood cells are largely unknown.Following our recent findings on erythrocyte and leukocyte trauma in VAD recipients, we decided to explore interactions between erythrocytes and leukocytes by perfusing human whole blood through two different types of VADs, HeartMate II (HMII) and HeartMate 3 (HM3), concurrently in their respective Blood Circulatory Loop (BCL). By using a flow cytometry assay, we found increasing association between erythrocytes and leukocytes as VADs propelled blood through their BCLs. This time-dependent intercellular association was shown by increasing concomitant events of stained CD235a (specifically expressed on erythrocytes) and stained CD45 (specifically expressed on leukocytes) in the CD235a+ population. Compared to CentriMag (CM), which served as a control, VADs produced significantly higher concomitant signals. The findings described in this study have opened the need for further studies on a novel path for generation of adverse events that are commonly observed in VAD recipients, notably infection, pump thrombosis, and ischemic stroke.http://www.sciencedirect.com/science/article/pii/S2405580825001724 |
| spellingShingle | Kylie M. Foster Ahmed M. El Banayosy Zheila Azartash-Namin Phillip Coghill James W. Long Edgar O'Rear Hendra Setiadi Blood perfusion through ventricular assist devices induces erythrocytes to interact with leukocytes Biochemistry and Biophysics Reports |
| title | Blood perfusion through ventricular assist devices induces erythrocytes to interact with leukocytes |
| title_full | Blood perfusion through ventricular assist devices induces erythrocytes to interact with leukocytes |
| title_fullStr | Blood perfusion through ventricular assist devices induces erythrocytes to interact with leukocytes |
| title_full_unstemmed | Blood perfusion through ventricular assist devices induces erythrocytes to interact with leukocytes |
| title_short | Blood perfusion through ventricular assist devices induces erythrocytes to interact with leukocytes |
| title_sort | blood perfusion through ventricular assist devices induces erythrocytes to interact with leukocytes |
| url | http://www.sciencedirect.com/science/article/pii/S2405580825001724 |
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