Impact of processing methods and amino acid supplementation of cassava root–leaf meal on performance and caeca microflora of broiler chicks

This study investigated the effects of processed cassava root-leaf meal (CRLM) on broiler performance and caecal microflora. A total of 240 Ross broiler chicks were randomly assigned to 10 dietary treatments in a 5 × 2 factorial design, comprising five energy sources: maize, sun-dried CRLM (SCRLM),...

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Main Authors: Wasiu Ajani Olayemi, Gabriel Adedotun Williams, Abimbola oladele Oso, Eunice Opeoluwa Omofunmilola
Format: Article
Language:English
Published: Universidade Federal de Goiás 2025-08-01
Series:Ciência Animal Brasileira
Online Access:https://revistas.ufg.br/vet/article/view/80893
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author Wasiu Ajani Olayemi
Gabriel Adedotun Williams
Abimbola oladele Oso
Eunice Opeoluwa Omofunmilola
author_facet Wasiu Ajani Olayemi
Gabriel Adedotun Williams
Abimbola oladele Oso
Eunice Opeoluwa Omofunmilola
author_sort Wasiu Ajani Olayemi
collection DOAJ
description This study investigated the effects of processed cassava root-leaf meal (CRLM) on broiler performance and caecal microflora. A total of 240 Ross broiler chicks were randomly assigned to 10 dietary treatments in a 5 × 2 factorial design, comprising five energy sources: maize, sun-dried CRLM (SCRLM), fermented CRLM (FCRLM), FCRLM with yeast (YFCRLM), and FCRLM with rumen filtrate (RFCRLM) and two amino acid levels (Ideal and National Research Council (NRC) 1994). The NRC diets included methionine and lysine, while the Ideal diets were supplemented with threonine and arginine. Each treatment was replicated three times with eight birds per replicate, and the trial lasted 28 days. Performance parameters were recorded weekly, and on day 28, selected birds were slaughtered for analysis of their gut microflora. The results indicated that diets formulated with Ideal amino acid levels significantly (P<0.05) improved weight gain (WG) and feed conversion ratio (FCR). The birds fed RFCRLM-based diets had significantly lower (P<0.05) feed intake (FI) and better FCR, comparable to those fed maize-based diets. Notably, the combination of RFCRLM and Ideal amino acids resulted in the lowest (P<0.05) FI and the best FCR overall. Microbial analysis revealed that maize-based diets resulted in higher (P<0.05) counts of Bacillus subtilis and Proteus mirabilis, whereas the CRLM-based diet, regardless of amino acid level, resulted in significantly lower (P<0.05) total bacterial counts (TBCs). In conclusion, supplementing broiler diets with Ideal amino acids enhanced growth performance, and RFCRLM effectively reduced feed intake without compromising FCR. Moreover, processed CRLM proved beneficial in lowering microbial load when used as an alternative to maize in broiler diets.
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spelling doaj-art-3b8d3316d8cc47c692d536a49f6cf54a2025-08-20T04:01:01ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912025-08-0126Impact of processing methods and amino acid supplementation of cassava root–leaf meal on performance and caeca microflora of broiler chicksWasiu Ajani Olayemi0Gabriel Adedotun Williams1Abimbola oladele Oso2Eunice Opeoluwa Omofunmilola3Yaba College of Technology, School of Agriculture, Lagos, NigeriaLagos State University, School of Agriculture, Lagos, NigeriaFederal University of Agriculture, College of Animal Science and Livestock Production, Ogun State, Abeokuta, NigeriaYaba College of Technology, School of Agriculture, Lagos, Nigeria This study investigated the effects of processed cassava root-leaf meal (CRLM) on broiler performance and caecal microflora. A total of 240 Ross broiler chicks were randomly assigned to 10 dietary treatments in a 5 × 2 factorial design, comprising five energy sources: maize, sun-dried CRLM (SCRLM), fermented CRLM (FCRLM), FCRLM with yeast (YFCRLM), and FCRLM with rumen filtrate (RFCRLM) and two amino acid levels (Ideal and National Research Council (NRC) 1994). The NRC diets included methionine and lysine, while the Ideal diets were supplemented with threonine and arginine. Each treatment was replicated three times with eight birds per replicate, and the trial lasted 28 days. Performance parameters were recorded weekly, and on day 28, selected birds were slaughtered for analysis of their gut microflora. The results indicated that diets formulated with Ideal amino acid levels significantly (P<0.05) improved weight gain (WG) and feed conversion ratio (FCR). The birds fed RFCRLM-based diets had significantly lower (P<0.05) feed intake (FI) and better FCR, comparable to those fed maize-based diets. Notably, the combination of RFCRLM and Ideal amino acids resulted in the lowest (P<0.05) FI and the best FCR overall. Microbial analysis revealed that maize-based diets resulted in higher (P<0.05) counts of Bacillus subtilis and Proteus mirabilis, whereas the CRLM-based diet, regardless of amino acid level, resulted in significantly lower (P<0.05) total bacterial counts (TBCs). In conclusion, supplementing broiler diets with Ideal amino acids enhanced growth performance, and RFCRLM effectively reduced feed intake without compromising FCR. Moreover, processed CRLM proved beneficial in lowering microbial load when used as an alternative to maize in broiler diets. https://revistas.ufg.br/vet/article/view/80893
spellingShingle Wasiu Ajani Olayemi
Gabriel Adedotun Williams
Abimbola oladele Oso
Eunice Opeoluwa Omofunmilola
Impact of processing methods and amino acid supplementation of cassava root–leaf meal on performance and caeca microflora of broiler chicks
Ciência Animal Brasileira
title Impact of processing methods and amino acid supplementation of cassava root–leaf meal on performance and caeca microflora of broiler chicks
title_full Impact of processing methods and amino acid supplementation of cassava root–leaf meal on performance and caeca microflora of broiler chicks
title_fullStr Impact of processing methods and amino acid supplementation of cassava root–leaf meal on performance and caeca microflora of broiler chicks
title_full_unstemmed Impact of processing methods and amino acid supplementation of cassava root–leaf meal on performance and caeca microflora of broiler chicks
title_short Impact of processing methods and amino acid supplementation of cassava root–leaf meal on performance and caeca microflora of broiler chicks
title_sort impact of processing methods and amino acid supplementation of cassava root leaf meal on performance and caeca microflora of broiler chicks
url https://revistas.ufg.br/vet/article/view/80893
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AT abimbolaoladeleoso impactofprocessingmethodsandaminoacidsupplementationofcassavarootleafmealonperformanceandcaecamicrofloraofbroilerchicks
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