An experimental study of the composition for medicament corneal crosslinking as a promising means for keratoconus treatment
The deterioration of the corneal framing function in keratoconus is largely due to the dystrophic process and the decrease of collagen structures crosslinking level. The purpose of the study is to perform a preclinical placebo controlled study of the safeness and effectiveness of Cornetex compound i...
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| Language: | Russian |
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Real Time Ltd
2018-10-01
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| Series: | Российский офтальмологический журнал |
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| Online Access: | https://roj.igb.ru/jour/article/view/123 |
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| author | E. N. Iomdina A. R. Korigodsky I. P. Khoroshilova-Maslova L. D. Andreeva N. Yu. Ignatieva |
| author_facet | E. N. Iomdina A. R. Korigodsky I. P. Khoroshilova-Maslova L. D. Andreeva N. Yu. Ignatieva |
| author_sort | E. N. Iomdina |
| collection | DOAJ |
| description | The deterioration of the corneal framing function in keratoconus is largely due to the dystrophic process and the decrease of collagen structures crosslinking level. The purpose of the study is to perform a preclinical placebo controlled study of the safeness and effectiveness of Cornetex compound instillations for medicament corneal crosslinking. Material and methods. The study included 37 Chinchilla rabbits (74 eyes). 33 rabbits received Cornetex solution instillations into one eye twice a day for 1 month (17 rabbits, 1st series) and for 3 months (16 rabbits, 2nd series). The active constituents of Cornetex include a complex of the basic amino acid salts in the form of succinates (residues of succinate acid) participating in collagen crosslinking formation. The placebo solution containing nonactive Cornetex constituents (Hydroxyethyl cellulose, benzalkonium chloride and water for injections) was instilled into fellow eyes. 4 fully intact rabbits (8 eyes) served as an independent control group. In vivo methods included biomicroscopy and examination with a Scheimpflug camera Galilei G2. 1 and 3 months after the instillation course, the eyes were enucleated and subjected to a morphological study, biomechanical testing of corneal samples (on the testing machine Autograph AGS-H). Corneal collagen crosslinking level was determined by differential scanning calorimetry using a Phoenix DSC 204 calorimeter. Results. Biomicroscopic and morphological studies revealed no pathological changes of ocular media and tissues. One month after the instillation course, the collagen crosslinking level of the cornea showed a 18 to 20 % increase, while its elasticity modulus showed a 1.8-fold increase with respect to the fellow eye, which is an evidence of the safety and high corneal reinforcement effect of the instillations. Conclusions. The outcome of the experimental study of Cornetex implies that it can be recommended for further clinical studies as a promising means for keratoconus treatment. For citations: Iomdina E.N., Korigodsky A.R., Khoroshilova-Maslova I.P., Andreeva L.D., Ignatieva N.Yu. An experimental study of the composition for medicament corneal crosslinking as a promising means for keratoconus treatment. Russian ophthalmological journal. 2017; 10 (4): 54-61. doi: 10.21516/2072-0076-2017-10-4-54-61 (In Russian). |
| format | Article |
| id | doaj-art-3b8779b12f7d4f94bfc3bd14fff2a8e2 |
| institution | DOAJ |
| issn | 2072-0076 2587-5760 |
| language | Russian |
| publishDate | 2018-10-01 |
| publisher | Real Time Ltd |
| record_format | Article |
| series | Российский офтальмологический журнал |
| spelling | doaj-art-3b8779b12f7d4f94bfc3bd14fff2a8e22025-08-20T03:19:25ZrusReal Time LtdРоссийский офтальмологический журнал2072-00762587-57602018-10-01104546110.21516/2072-0076-2017-10-4-54-61123An experimental study of the composition for medicament corneal crosslinking as a promising means for keratoconus treatmentE. N. Iomdina0A. R. Korigodsky1I. P. Khoroshilova-Maslova2L. D. Andreeva3N. Yu. Ignatieva4Moscow Helmholtz Research Institute of Eye DiseasesHighBeTech LtdMoscow Helmholtz Research Institute of Eye DiseasesMoscow Helmholtz Research Institute of Eye DiseasesLomonosov Moscow State UniversityThe deterioration of the corneal framing function in keratoconus is largely due to the dystrophic process and the decrease of collagen structures crosslinking level. The purpose of the study is to perform a preclinical placebo controlled study of the safeness and effectiveness of Cornetex compound instillations for medicament corneal crosslinking. Material and methods. The study included 37 Chinchilla rabbits (74 eyes). 33 rabbits received Cornetex solution instillations into one eye twice a day for 1 month (17 rabbits, 1st series) and for 3 months (16 rabbits, 2nd series). The active constituents of Cornetex include a complex of the basic amino acid salts in the form of succinates (residues of succinate acid) participating in collagen crosslinking formation. The placebo solution containing nonactive Cornetex constituents (Hydroxyethyl cellulose, benzalkonium chloride and water for injections) was instilled into fellow eyes. 4 fully intact rabbits (8 eyes) served as an independent control group. In vivo methods included biomicroscopy and examination with a Scheimpflug camera Galilei G2. 1 and 3 months after the instillation course, the eyes were enucleated and subjected to a morphological study, biomechanical testing of corneal samples (on the testing machine Autograph AGS-H). Corneal collagen crosslinking level was determined by differential scanning calorimetry using a Phoenix DSC 204 calorimeter. Results. Biomicroscopic and morphological studies revealed no pathological changes of ocular media and tissues. One month after the instillation course, the collagen crosslinking level of the cornea showed a 18 to 20 % increase, while its elasticity modulus showed a 1.8-fold increase with respect to the fellow eye, which is an evidence of the safety and high corneal reinforcement effect of the instillations. Conclusions. The outcome of the experimental study of Cornetex implies that it can be recommended for further clinical studies as a promising means for keratoconus treatment. For citations: Iomdina E.N., Korigodsky A.R., Khoroshilova-Maslova I.P., Andreeva L.D., Ignatieva N.Yu. An experimental study of the composition for medicament corneal crosslinking as a promising means for keratoconus treatment. Russian ophthalmological journal. 2017; 10 (4): 54-61. doi: 10.21516/2072-0076-2017-10-4-54-61 (In Russian).https://roj.igb.ru/jour/article/view/123роговицапоперечные сшивкиосновные аминокислотысукцинатымодуль упругостикератоконусcrosslinkingbasic amino acidssuccinateselasticity moduluskeratoconus |
| spellingShingle | E. N. Iomdina A. R. Korigodsky I. P. Khoroshilova-Maslova L. D. Andreeva N. Yu. Ignatieva An experimental study of the composition for medicament corneal crosslinking as a promising means for keratoconus treatment Российский офтальмологический журнал роговица поперечные сшивки основные аминокислоты сукцинаты модуль упругости кератоконус crosslinking basic amino acids succinates elasticity modulus keratoconus |
| title | An experimental study of the composition for medicament corneal crosslinking as a promising means for keratoconus treatment |
| title_full | An experimental study of the composition for medicament corneal crosslinking as a promising means for keratoconus treatment |
| title_fullStr | An experimental study of the composition for medicament corneal crosslinking as a promising means for keratoconus treatment |
| title_full_unstemmed | An experimental study of the composition for medicament corneal crosslinking as a promising means for keratoconus treatment |
| title_short | An experimental study of the composition for medicament corneal crosslinking as a promising means for keratoconus treatment |
| title_sort | experimental study of the composition for medicament corneal crosslinking as a promising means for keratoconus treatment |
| topic | роговица поперечные сшивки основные аминокислоты сукцинаты модуль упругости кератоконус crosslinking basic amino acids succinates elasticity modulus keratoconus |
| url | https://roj.igb.ru/jour/article/view/123 |
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