An Experimental Study on [125I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment
Objective. To evaluate the diagnostic efficacy of MDA-MB-231 triple-negative breast cancer with 125I-labeled pHLIP (Var7) by single-photon emission computed tomography/computed tomography (SPECT/CT) imaging. Methods. The binding fraction of [125I]I-pHLIP (Var7) and MDA-MB-231 cells was measured at p...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2021-01-01
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| Series: | Molecular Imaging |
| Online Access: | http://dx.doi.org/10.1155/2021/5565932 |
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| _version_ | 1846097966372225024 |
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| author | Mingming Yu Yanqin Sun Guangjie Yang Zhenguang Wang |
| author_facet | Mingming Yu Yanqin Sun Guangjie Yang Zhenguang Wang |
| author_sort | Mingming Yu |
| collection | DOAJ |
| description | Objective. To evaluate the diagnostic efficacy of MDA-MB-231 triple-negative breast cancer with 125I-labeled pHLIP (Var7) by single-photon emission computed tomography/computed tomography (SPECT/CT) imaging. Methods. The binding fraction of [125I]I-pHLIP (Var7) and MDA-MB-231 cells was measured at pH 7.4 and pH 6.0, and tumor-bearing mice were subjected to small-animal SPECT/CT imaging studies. Results. At pH=6.0, the binding fractions of [125I]I-pHLIP (Var7) and MDA-MB-231 cells at 10 min, 40 min, 1 h, and 2 h were 1.9±0.1%, 3.5±0.1%, 6.3±0.8%, and 6.6±0.3%, respectively. At pH=7.4, there was no measured binding between [125I]I-pHLIP (Var7) and MDA-MB-231 cells. Small-animal SPECT/CT imaging showed clearly visible tumors at 1 and 2 h after injection. Conclusions. [125I]I-pHLIP (Var7) could bind to MDA-MB-231 cells in an acidic environment, and small-animal SPECT/CT imaging showed clear tumors at 1 and 2 h after probe injection. |
| format | Article |
| id | doaj-art-3b84e698f5f646ceb00a2c5b0e69a95d |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-3b84e698f5f646ceb00a2c5b0e69a95d2025-01-02T02:57:18ZengSAGE PublishingMolecular Imaging1536-01212021-01-01202110.1155/2021/55659325565932An Experimental Study on [125I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor MicroenvironmentMingming Yu0Yanqin Sun1Guangjie Yang2Zhenguang Wang3Department of Nuclear Medicine, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Nuclear Medicine, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Nuclear Medicine, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Nuclear Medicine, The Affiliated Hospital of Qingdao University, Qingdao, ChinaObjective. To evaluate the diagnostic efficacy of MDA-MB-231 triple-negative breast cancer with 125I-labeled pHLIP (Var7) by single-photon emission computed tomography/computed tomography (SPECT/CT) imaging. Methods. The binding fraction of [125I]I-pHLIP (Var7) and MDA-MB-231 cells was measured at pH 7.4 and pH 6.0, and tumor-bearing mice were subjected to small-animal SPECT/CT imaging studies. Results. At pH=6.0, the binding fractions of [125I]I-pHLIP (Var7) and MDA-MB-231 cells at 10 min, 40 min, 1 h, and 2 h were 1.9±0.1%, 3.5±0.1%, 6.3±0.8%, and 6.6±0.3%, respectively. At pH=7.4, there was no measured binding between [125I]I-pHLIP (Var7) and MDA-MB-231 cells. Small-animal SPECT/CT imaging showed clearly visible tumors at 1 and 2 h after injection. Conclusions. [125I]I-pHLIP (Var7) could bind to MDA-MB-231 cells in an acidic environment, and small-animal SPECT/CT imaging showed clear tumors at 1 and 2 h after probe injection.http://dx.doi.org/10.1155/2021/5565932 |
| spellingShingle | Mingming Yu Yanqin Sun Guangjie Yang Zhenguang Wang An Experimental Study on [125I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment Molecular Imaging |
| title | An Experimental Study on [125I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment |
| title_full | An Experimental Study on [125I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment |
| title_fullStr | An Experimental Study on [125I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment |
| title_full_unstemmed | An Experimental Study on [125I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment |
| title_short | An Experimental Study on [125I]I-pHLIP (Var7) for SPECT/CT Imaging of an MDA-MB-231 Triple-Negative Breast Cancer Mouse Model by Targeting the Tumor Microenvironment |
| title_sort | experimental study on 125i i phlip var7 for spect ct imaging of an mda mb 231 triple negative breast cancer mouse model by targeting the tumor microenvironment |
| url | http://dx.doi.org/10.1155/2021/5565932 |
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