Prophylactic Antioxidant Potential of Gallic Acid in Murine Model of Sepsis
Present study is to investigate the effect of Gallic acid pretreatment on survival of septic animals and oxidative stress in different organs like lungs, liver, kidney, spleen, and vascular dysfunction of mice. Sepsis was induced by cecal ligation and puncture (CLP) in healthy adult male albino mice...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/580320 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849304618114220032 |
|---|---|
| author | Harikesh Maurya Vaishali Mangal Sanjay Gandhi Kathiresan Prabhu Kathiresan Ponnudurai |
| author_facet | Harikesh Maurya Vaishali Mangal Sanjay Gandhi Kathiresan Prabhu Kathiresan Ponnudurai |
| author_sort | Harikesh Maurya |
| collection | DOAJ |
| description | Present study is to investigate the effect of Gallic acid pretreatment on survival of
septic animals and oxidative stress in different organs like lungs, liver, kidney, spleen, and
vascular dysfunction of mice. Sepsis was induced by cecal ligation and puncture (CLP) in
healthy adult male albino mice (25–30 g) and was divided into 3 groups each consisting of 6 animals, that is, sham-operated (SO group (Group I), SO + sepsis (Group II), and Gallic acid + sepsis (Group III)). Group III animals were pretreated with Gallic acid at the dose rate of 20 mg/kg body weight for 2 days before induction of sepsis. Animals were sacrificed on 8th day and the tissue samples were obtained for further investigation on lipid peroxidation (LPO), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH). Gallic acid pretreatment significant (P<0.05) reduces kidney, spleen, liver, and lungs’ malondialdehyde level in septic mice. However, it fails to improve reduced glutathione level in all given organs, while, Gallic acid pretreated mice showed significant improvement in SOD activity of kidney and spleen when compared to septic mice. Finally, the beneficial effects of Gallic acid pretreatment in sepsis are evident from the observations that Gallic acid partially restored SOD and catalase activity and completely reversed lipid peroxidation. Further studies are required to find out the possible mechanisms underlying the beneficial effects of Gallic acid on large population. |
| format | Article |
| id | doaj-art-3b83f3156a8b499fb1cf3e89c054775c |
| institution | Kabale University |
| issn | 2090-8040 2042-0099 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-3b83f3156a8b499fb1cf3e89c054775c2025-08-20T03:55:41ZengWileyInternational Journal of Inflammation2090-80402042-00992014-01-01201410.1155/2014/580320580320Prophylactic Antioxidant Potential of Gallic Acid in Murine Model of SepsisHarikesh Maurya0Vaishali Mangal1Sanjay Gandhi2Kathiresan Prabhu3Kathiresan Ponnudurai4Siddhartha Institute of Pharmacy, Near I.T. Park, Sahastradhara Road, Dehradun, IndiaSiddhartha Institute of Pharmacy, Near I.T. Park, Sahastradhara Road, Dehradun, IndiaCity Heart Centre, 19 Cross Road, Dehradun 248001, IndiaNova College Pharmacy, Vegavaram, Jangareddigudem, Andhra Pradesh 534447, IndiaNova College Pharmacy, Vegavaram, Jangareddigudem, Andhra Pradesh 534447, IndiaPresent study is to investigate the effect of Gallic acid pretreatment on survival of septic animals and oxidative stress in different organs like lungs, liver, kidney, spleen, and vascular dysfunction of mice. Sepsis was induced by cecal ligation and puncture (CLP) in healthy adult male albino mice (25–30 g) and was divided into 3 groups each consisting of 6 animals, that is, sham-operated (SO group (Group I), SO + sepsis (Group II), and Gallic acid + sepsis (Group III)). Group III animals were pretreated with Gallic acid at the dose rate of 20 mg/kg body weight for 2 days before induction of sepsis. Animals were sacrificed on 8th day and the tissue samples were obtained for further investigation on lipid peroxidation (LPO), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH). Gallic acid pretreatment significant (P<0.05) reduces kidney, spleen, liver, and lungs’ malondialdehyde level in septic mice. However, it fails to improve reduced glutathione level in all given organs, while, Gallic acid pretreated mice showed significant improvement in SOD activity of kidney and spleen when compared to septic mice. Finally, the beneficial effects of Gallic acid pretreatment in sepsis are evident from the observations that Gallic acid partially restored SOD and catalase activity and completely reversed lipid peroxidation. Further studies are required to find out the possible mechanisms underlying the beneficial effects of Gallic acid on large population.http://dx.doi.org/10.1155/2014/580320 |
| spellingShingle | Harikesh Maurya Vaishali Mangal Sanjay Gandhi Kathiresan Prabhu Kathiresan Ponnudurai Prophylactic Antioxidant Potential of Gallic Acid in Murine Model of Sepsis International Journal of Inflammation |
| title | Prophylactic Antioxidant Potential of Gallic Acid in Murine Model of Sepsis |
| title_full | Prophylactic Antioxidant Potential of Gallic Acid in Murine Model of Sepsis |
| title_fullStr | Prophylactic Antioxidant Potential of Gallic Acid in Murine Model of Sepsis |
| title_full_unstemmed | Prophylactic Antioxidant Potential of Gallic Acid in Murine Model of Sepsis |
| title_short | Prophylactic Antioxidant Potential of Gallic Acid in Murine Model of Sepsis |
| title_sort | prophylactic antioxidant potential of gallic acid in murine model of sepsis |
| url | http://dx.doi.org/10.1155/2014/580320 |
| work_keys_str_mv | AT harikeshmaurya prophylacticantioxidantpotentialofgallicacidinmurinemodelofsepsis AT vaishalimangal prophylacticantioxidantpotentialofgallicacidinmurinemodelofsepsis AT sanjaygandhi prophylacticantioxidantpotentialofgallicacidinmurinemodelofsepsis AT kathiresanprabhu prophylacticantioxidantpotentialofgallicacidinmurinemodelofsepsis AT kathiresanponnudurai prophylacticantioxidantpotentialofgallicacidinmurinemodelofsepsis |