RBAP48 facilitates the oral squamous cell carcinoma process in an androgen receptor-dependent and independent manners

Abstract Oral squamous cell carcinoma (OSCC) progresses from epithelial cell proliferation to malignancy. Given the higher proportion of male patients compared to female patients, the androgen signaling pathway is believed to play a significant role in promoting epithelial cell proliferation. Howeve...

Full description

Saved in:
Bibliographic Details
Main Authors: Xue Wang, Guangqi Yan, Hao Li, Chunyu Wang, Ye Kang, Shengli Wang, Wei Liu, Lin Lin, Renlong Zou, Kai Zeng, Manlin Wang, Ruina Luan, Baosheng Zhou, Yu Bai, Dongjun Yang, Bolin Ning, Ge Sun, Yue Zhao
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-08215-4
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850231415136845824
author Xue Wang
Guangqi Yan
Hao Li
Chunyu Wang
Ye Kang
Shengli Wang
Wei Liu
Lin Lin
Renlong Zou
Kai Zeng
Manlin Wang
Ruina Luan
Baosheng Zhou
Yu Bai
Dongjun Yang
Bolin Ning
Ge Sun
Yue Zhao
author_facet Xue Wang
Guangqi Yan
Hao Li
Chunyu Wang
Ye Kang
Shengli Wang
Wei Liu
Lin Lin
Renlong Zou
Kai Zeng
Manlin Wang
Ruina Luan
Baosheng Zhou
Yu Bai
Dongjun Yang
Bolin Ning
Ge Sun
Yue Zhao
author_sort Xue Wang
collection DOAJ
description Abstract Oral squamous cell carcinoma (OSCC) progresses from epithelial cell proliferation to malignancy. Given the higher proportion of male patients compared to female patients, the androgen signaling pathway is believed to play a significant role in promoting epithelial cell proliferation. However, the underlying molecular mechanisms remain unclear. Here, we identified RBAP48 as a novel androgen receptor (AR) co-activator in OSCC cells. Our results show that RBAP48 was highly expressed in OSCC tumor tissues from patients with a poor prognosis. Further, RBAP48 knockdown decreased genome-wide oncogene transcription. RBAP48 and AR interacted to activate CCND1 and RAB31 transcription, and upregulated RELA and CCNE1 mRNA expression through an AR-independent pathway. Additionally, RBAP48 promoted OSCC cell proliferation and was involved in the cellular response to drugs and external compounds in vitro, ultimately driving cancer progression. Our results indicate that RBAP48 is a novel oncogene and a promising target for predicting and treating OSCC progression.
format Article
id doaj-art-3b73d22c7c6e4450a8b103be343a3edd
institution OA Journals
issn 2399-3642
language English
publishDate 2025-05-01
publisher Nature Portfolio
record_format Article
series Communications Biology
spelling doaj-art-3b73d22c7c6e4450a8b103be343a3edd2025-08-20T02:03:32ZengNature PortfolioCommunications Biology2399-36422025-05-018111510.1038/s42003-025-08215-4RBAP48 facilitates the oral squamous cell carcinoma process in an androgen receptor-dependent and independent mannersXue Wang0Guangqi Yan1Hao Li2Chunyu Wang3Ye Kang4Shengli Wang5Wei Liu6Lin Lin7Renlong Zou8Kai Zeng9Manlin Wang10Ruina Luan11Baosheng Zhou12Yu Bai13Dongjun Yang14Bolin Ning15Ge Sun16Yue Zhao17Department of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Oral and Maxillofacial Surgery, School of Stomatology, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of pathology, Shengjing hospital of China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityDepartment of Cell Biology, Key laboratory of Cell Biology, Ministry of Public Health, and Key laboratory of Medical Cell Biology, Ministry of Education, School of Life Sciences, China Medical UniversityAbstract Oral squamous cell carcinoma (OSCC) progresses from epithelial cell proliferation to malignancy. Given the higher proportion of male patients compared to female patients, the androgen signaling pathway is believed to play a significant role in promoting epithelial cell proliferation. However, the underlying molecular mechanisms remain unclear. Here, we identified RBAP48 as a novel androgen receptor (AR) co-activator in OSCC cells. Our results show that RBAP48 was highly expressed in OSCC tumor tissues from patients with a poor prognosis. Further, RBAP48 knockdown decreased genome-wide oncogene transcription. RBAP48 and AR interacted to activate CCND1 and RAB31 transcription, and upregulated RELA and CCNE1 mRNA expression through an AR-independent pathway. Additionally, RBAP48 promoted OSCC cell proliferation and was involved in the cellular response to drugs and external compounds in vitro, ultimately driving cancer progression. Our results indicate that RBAP48 is a novel oncogene and a promising target for predicting and treating OSCC progression.https://doi.org/10.1038/s42003-025-08215-4
spellingShingle Xue Wang
Guangqi Yan
Hao Li
Chunyu Wang
Ye Kang
Shengli Wang
Wei Liu
Lin Lin
Renlong Zou
Kai Zeng
Manlin Wang
Ruina Luan
Baosheng Zhou
Yu Bai
Dongjun Yang
Bolin Ning
Ge Sun
Yue Zhao
RBAP48 facilitates the oral squamous cell carcinoma process in an androgen receptor-dependent and independent manners
Communications Biology
title RBAP48 facilitates the oral squamous cell carcinoma process in an androgen receptor-dependent and independent manners
title_full RBAP48 facilitates the oral squamous cell carcinoma process in an androgen receptor-dependent and independent manners
title_fullStr RBAP48 facilitates the oral squamous cell carcinoma process in an androgen receptor-dependent and independent manners
title_full_unstemmed RBAP48 facilitates the oral squamous cell carcinoma process in an androgen receptor-dependent and independent manners
title_short RBAP48 facilitates the oral squamous cell carcinoma process in an androgen receptor-dependent and independent manners
title_sort rbap48 facilitates the oral squamous cell carcinoma process in an androgen receptor dependent and independent manners
url https://doi.org/10.1038/s42003-025-08215-4
work_keys_str_mv AT xuewang rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT guangqiyan rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT haoli rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT chunyuwang rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT yekang rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT shengliwang rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT weiliu rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT linlin rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT renlongzou rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT kaizeng rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT manlinwang rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT ruinaluan rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT baoshengzhou rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT yubai rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT dongjunyang rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT bolinning rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT gesun rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners
AT yuezhao rbap48facilitatestheoralsquamouscellcarcinomaprocessinanandrogenreceptordependentandindependentmanners