CyTOF mass cytometry reveals phenotypically distinct human blood neutrophil populations differentially correlated with melanoma stage
Background Understanding neutrophil heterogeneity and its relationship to disease progression has become a recent focus of cancer research. Indeed, several studies have identified neutrophil subpopulations associated with protumoral or antitumoral functions. However, this work has been hindered by a...
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| Format: | Article |
| Language: | English |
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BMJ Publishing Group
2020-10-01
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| Series: | Journal for ImmunoTherapy of Cancer |
| Online Access: | https://jitc.bmj.com/content/8/2/e000473.full |
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| author | Tobias Eggert Christian Hermann Ottensmeier Catherine C Hedrick Pandurangan Vijayanand Yanfang Peipei Zhu Daniel J Araujo |
| author_facet | Tobias Eggert Christian Hermann Ottensmeier Catherine C Hedrick Pandurangan Vijayanand Yanfang Peipei Zhu Daniel J Araujo |
| author_sort | Tobias Eggert |
| collection | DOAJ |
| description | Background Understanding neutrophil heterogeneity and its relationship to disease progression has become a recent focus of cancer research. Indeed, several studies have identified neutrophil subpopulations associated with protumoral or antitumoral functions. However, this work has been hindered by a lack of widely accepted markers with which to define neutrophil subpopulations.Methods To identify markers of neutrophil heterogeneity in cancer, we used single-cell cytometry by time-of-flight (CyTOF) coupled with high-dimensional analysis on blood samples from treatment-naïve patients with melanoma.Results Our efforts allowed us to identify seven blood neutrophil clusters, including two previously identified individual populations. Interrogation of these neutrophil subpopulations revealed a positive trend between specific clusters and disease stage. Finally, we recapitulated these seven blood neutrophil populations via flow cytometry and found that they exhibited diverse capacities for phagocytosis and reactive oxygen species production in vitro.Conclusions Our data provide a refined consensus on neutrophil heterogeneity markers, enabling a prospective functional evaluation in patients with solid tumors. |
| format | Article |
| id | doaj-art-3b6e8973d2c147aca70a04510eb2cd51 |
| institution | Kabale University |
| issn | 2051-1426 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | Journal for ImmunoTherapy of Cancer |
| spelling | doaj-art-3b6e8973d2c147aca70a04510eb2cd512024-11-10T16:15:07ZengBMJ Publishing GroupJournal for ImmunoTherapy of Cancer2051-14262020-10-018210.1136/jitc-2019-000473CyTOF mass cytometry reveals phenotypically distinct human blood neutrophil populations differentially correlated with melanoma stageTobias Eggert0Christian Hermann Ottensmeier1Catherine C Hedrick2Pandurangan Vijayanand3Yanfang Peipei Zhu4Daniel J Araujo51 Inflammation Biology, La Jolla Institute for Immunology, La Jolla, California, USA3 School of Cancer Sciences, University of Southampton Faculty of Medicine, Southampton, UK1 Inflammation Biology, La Jolla Institute for Immunology, La Jolla, California, USALa Jolla Institute for Immunology, La Jolla, California, USA1 Inflammation Biology, La Jolla Institute for Immunology, La Jolla, California, USA1 Inflammation Biology, La Jolla Institute for Immunology, La Jolla, California, USABackground Understanding neutrophil heterogeneity and its relationship to disease progression has become a recent focus of cancer research. Indeed, several studies have identified neutrophil subpopulations associated with protumoral or antitumoral functions. However, this work has been hindered by a lack of widely accepted markers with which to define neutrophil subpopulations.Methods To identify markers of neutrophil heterogeneity in cancer, we used single-cell cytometry by time-of-flight (CyTOF) coupled with high-dimensional analysis on blood samples from treatment-naïve patients with melanoma.Results Our efforts allowed us to identify seven blood neutrophil clusters, including two previously identified individual populations. Interrogation of these neutrophil subpopulations revealed a positive trend between specific clusters and disease stage. Finally, we recapitulated these seven blood neutrophil populations via flow cytometry and found that they exhibited diverse capacities for phagocytosis and reactive oxygen species production in vitro.Conclusions Our data provide a refined consensus on neutrophil heterogeneity markers, enabling a prospective functional evaluation in patients with solid tumors.https://jitc.bmj.com/content/8/2/e000473.full |
| spellingShingle | Tobias Eggert Christian Hermann Ottensmeier Catherine C Hedrick Pandurangan Vijayanand Yanfang Peipei Zhu Daniel J Araujo CyTOF mass cytometry reveals phenotypically distinct human blood neutrophil populations differentially correlated with melanoma stage Journal for ImmunoTherapy of Cancer |
| title | CyTOF mass cytometry reveals phenotypically distinct human blood neutrophil populations differentially correlated with melanoma stage |
| title_full | CyTOF mass cytometry reveals phenotypically distinct human blood neutrophil populations differentially correlated with melanoma stage |
| title_fullStr | CyTOF mass cytometry reveals phenotypically distinct human blood neutrophil populations differentially correlated with melanoma stage |
| title_full_unstemmed | CyTOF mass cytometry reveals phenotypically distinct human blood neutrophil populations differentially correlated with melanoma stage |
| title_short | CyTOF mass cytometry reveals phenotypically distinct human blood neutrophil populations differentially correlated with melanoma stage |
| title_sort | cytof mass cytometry reveals phenotypically distinct human blood neutrophil populations differentially correlated with melanoma stage |
| url | https://jitc.bmj.com/content/8/2/e000473.full |
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