DEVELOPMENT AND BASIC FEATURES OF INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF ANTIBODIES AGAINST PORCINE EPIDEMIC DIARRHEA
Spread of porcine epidemic diarrhea and its increased threat for the pig industry necessitate development of advanced techniques for the disease diagnosis. Use of recombinant antigens of the disease agent seems prospective. The recombinant antigen-based indirect enzyme-linked immunosorbent assay has...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Da Vinci Media
2018-10-01
|
| Series: | Ветеринария сегодня |
| Subjects: | |
| Online Access: | https://veterinary.arriah.ru/jour/article/view/368 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832086718717624320 |
|---|---|
| author | A. V. Kanshina A. S. Yakovleva Ye. S. Orlova A. V. Scherbakov |
| author_facet | A. V. Kanshina A. S. Yakovleva Ye. S. Orlova A. V. Scherbakov |
| author_sort | A. V. Kanshina |
| collection | DOAJ |
| description | Spread of porcine epidemic diarrhea and its increased threat for the pig industry necessitate development of advanced techniques for the disease diagnosis. Use of recombinant antigens of the disease agent seems prospective. The recombinant antigen-based indirect enzyme-linked immunosorbent assay has been developed for the detection of antibodies against porcine epidemic diarrhea virus. The research performed allowed for determination of all necessary test conditions. Basic features of the developed method were determined during its validation. The test precision was above 90% pursuant to its repeatability and reproducibility, diagnostic specificity of the test amounted to 99.47%, and its sensitivity as compared to commercial ID Screen PEDV Indirect (IDvet, France) was 92% with very good compatibility of the two tests (k-criterion – 0.88) – 92%. The test-system demonstrates high stability upon the change of the key test component. |
| format | Article |
| id | doaj-art-3b61e0720e104c939f5eb241d7451160 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2018-10-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-3b61e0720e104c939f5eb241d74511602025-02-06T09:52:07ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-10-010381210.29326/2304-196X-2018-3-26-8-12366DEVELOPMENT AND BASIC FEATURES OF INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF ANTIBODIES AGAINST PORCINE EPIDEMIC DIARRHEAA. V. Kanshina0A. S. Yakovleva1Ye. S. Orlova2A. V. Scherbakov3FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirSpread of porcine epidemic diarrhea and its increased threat for the pig industry necessitate development of advanced techniques for the disease diagnosis. Use of recombinant antigens of the disease agent seems prospective. The recombinant antigen-based indirect enzyme-linked immunosorbent assay has been developed for the detection of antibodies against porcine epidemic diarrhea virus. The research performed allowed for determination of all necessary test conditions. Basic features of the developed method were determined during its validation. The test precision was above 90% pursuant to its repeatability and reproducibility, diagnostic specificity of the test amounted to 99.47%, and its sensitivity as compared to commercial ID Screen PEDV Indirect (IDvet, France) was 92% with very good compatibility of the two tests (k-criterion – 0.88) – 92%. The test-system demonstrates high stability upon the change of the key test component.https://veterinary.arriah.ru/jour/article/view/368porcine epidemic diarrheaantibodiesenzyme-linked immunozorbent assay |
| spellingShingle | A. V. Kanshina A. S. Yakovleva Ye. S. Orlova A. V. Scherbakov DEVELOPMENT AND BASIC FEATURES OF INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF ANTIBODIES AGAINST PORCINE EPIDEMIC DIARRHEA Ветеринария сегодня porcine epidemic diarrhea antibodies enzyme-linked immunozorbent assay |
| title | DEVELOPMENT AND BASIC FEATURES OF INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF ANTIBODIES AGAINST PORCINE EPIDEMIC DIARRHEA |
| title_full | DEVELOPMENT AND BASIC FEATURES OF INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF ANTIBODIES AGAINST PORCINE EPIDEMIC DIARRHEA |
| title_fullStr | DEVELOPMENT AND BASIC FEATURES OF INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF ANTIBODIES AGAINST PORCINE EPIDEMIC DIARRHEA |
| title_full_unstemmed | DEVELOPMENT AND BASIC FEATURES OF INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF ANTIBODIES AGAINST PORCINE EPIDEMIC DIARRHEA |
| title_short | DEVELOPMENT AND BASIC FEATURES OF INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF ANTIBODIES AGAINST PORCINE EPIDEMIC DIARRHEA |
| title_sort | development and basic features of indirect enzyme linked immunosorbent assay for detection of antibodies against porcine epidemic diarrhea |
| topic | porcine epidemic diarrhea antibodies enzyme-linked immunozorbent assay |
| url | https://veterinary.arriah.ru/jour/article/view/368 |
| work_keys_str_mv | AT avkanshina developmentandbasicfeaturesofindirectenzymelinkedimmunosorbentassayfordetectionofantibodiesagainstporcineepidemicdiarrhea AT asyakovleva developmentandbasicfeaturesofindirectenzymelinkedimmunosorbentassayfordetectionofantibodiesagainstporcineepidemicdiarrhea AT yesorlova developmentandbasicfeaturesofindirectenzymelinkedimmunosorbentassayfordetectionofantibodiesagainstporcineepidemicdiarrhea AT avscherbakov developmentandbasicfeaturesofindirectenzymelinkedimmunosorbentassayfordetectionofantibodiesagainstporcineepidemicdiarrhea |