TIM3 and CTLA4 immune checkpoint polymorphisms are associated with acute myeloid leukemia in Saudi Arabia

Background Immune checkpoints are receptors on the surface of T cells that function crucially in suppressing the immune response, and they are implicated in autoimmunity and cancer diseases.Aim The present study aimed to investigate the relationship between functional single nucleotide polymorphisms...

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Main Authors: Mashael Alqahtani, Ali Aljuaimlani, Jameel Al-Tamimi, Suliman Alomar, Lamjed Mansour
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Hematology
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Online Access:https://www.tandfonline.com/doi/10.1080/16078454.2024.2329024
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author Mashael Alqahtani
Ali Aljuaimlani
Jameel Al-Tamimi
Suliman Alomar
Lamjed Mansour
author_facet Mashael Alqahtani
Ali Aljuaimlani
Jameel Al-Tamimi
Suliman Alomar
Lamjed Mansour
author_sort Mashael Alqahtani
collection DOAJ
description Background Immune checkpoints are receptors on the surface of T cells that function crucially in suppressing the immune response, and they are implicated in autoimmunity and cancer diseases.Aim The present study aimed to investigate the relationship between functional single nucleotide polymorphisms (SNPs) of two immune checkpoint molecules, CTLA-4 and TIM-3, and acute myeloid leukemia (AML) in a Saudi population.Methods Two SNPs in CTLA-4 (rs231775, A > G) and TIM-3 (rs10515746, A > C) were genotyped in 229 subjects, including 98 patients and 131 healthy controls, from the Saudi population using TaqMan assay methods. Differential expression of these two genes was performed using in silico analysis.Results An association was found between polymorphisms in TIM-3 (OR: 6.01; 95% CI: 3.99–9.05, P < 0.0001) and the risk of AML. Inversely, the rs231775 SNP in the CTLA-4 gene was found to protect against AML in allelic, dominant, and additive models (P < 0.05). A significantly higher expression of TIM-3 in the blood of individuals with AML was observed.Conclusion This is the first study focusing on single nucleotide polymorphisms (SNPs) for CTLA-4 and TIM-3 in acute myeloid leukemia patients in a Saudi community and could be a potential new prognostic factor for this disease.
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spelling doaj-art-3b5170d4663d4d33bdaa0805795db7e22025-08-20T02:34:31ZengTaylor & Francis GroupHematology1607-84542024-12-0129110.1080/16078454.2024.2329024TIM3 and CTLA4 immune checkpoint polymorphisms are associated with acute myeloid leukemia in Saudi ArabiaMashael Alqahtani0Ali Aljuaimlani1Jameel Al-Tamimi2Suliman Alomar3Lamjed Mansour4Department of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaBackground Immune checkpoints are receptors on the surface of T cells that function crucially in suppressing the immune response, and they are implicated in autoimmunity and cancer diseases.Aim The present study aimed to investigate the relationship between functional single nucleotide polymorphisms (SNPs) of two immune checkpoint molecules, CTLA-4 and TIM-3, and acute myeloid leukemia (AML) in a Saudi population.Methods Two SNPs in CTLA-4 (rs231775, A > G) and TIM-3 (rs10515746, A > C) were genotyped in 229 subjects, including 98 patients and 131 healthy controls, from the Saudi population using TaqMan assay methods. Differential expression of these two genes was performed using in silico analysis.Results An association was found between polymorphisms in TIM-3 (OR: 6.01; 95% CI: 3.99–9.05, P < 0.0001) and the risk of AML. Inversely, the rs231775 SNP in the CTLA-4 gene was found to protect against AML in allelic, dominant, and additive models (P < 0.05). A significantly higher expression of TIM-3 in the blood of individuals with AML was observed.Conclusion This is the first study focusing on single nucleotide polymorphisms (SNPs) for CTLA-4 and TIM-3 in acute myeloid leukemia patients in a Saudi community and could be a potential new prognostic factor for this disease.https://www.tandfonline.com/doi/10.1080/16078454.2024.2329024Hematological malignancyCTLA-4TIM-3‌Immune checkpoint moleculessingle nucleotide polymorphismacute myeloid leukemia
spellingShingle Mashael Alqahtani
Ali Aljuaimlani
Jameel Al-Tamimi
Suliman Alomar
Lamjed Mansour
TIM3 and CTLA4 immune checkpoint polymorphisms are associated with acute myeloid leukemia in Saudi Arabia
Hematology
Hematological malignancy
CTLA-4
TIM-3‌
Immune checkpoint molecules
single nucleotide polymorphism
acute myeloid leukemia
title TIM3 and CTLA4 immune checkpoint polymorphisms are associated with acute myeloid leukemia in Saudi Arabia
title_full TIM3 and CTLA4 immune checkpoint polymorphisms are associated with acute myeloid leukemia in Saudi Arabia
title_fullStr TIM3 and CTLA4 immune checkpoint polymorphisms are associated with acute myeloid leukemia in Saudi Arabia
title_full_unstemmed TIM3 and CTLA4 immune checkpoint polymorphisms are associated with acute myeloid leukemia in Saudi Arabia
title_short TIM3 and CTLA4 immune checkpoint polymorphisms are associated with acute myeloid leukemia in Saudi Arabia
title_sort tim3 and ctla4 immune checkpoint polymorphisms are associated with acute myeloid leukemia in saudi arabia
topic Hematological malignancy
CTLA-4
TIM-3‌
Immune checkpoint molecules
single nucleotide polymorphism
acute myeloid leukemia
url https://www.tandfonline.com/doi/10.1080/16078454.2024.2329024
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