Synthesis and Performance of Hybrid Hydrogels Loaded with Methylene Blue and Its Use for Antimicrobial Photodynamic Inactivation

Development and characterization of hybrid hydrogels loaded with methylene blue, which are designed to apply for photodynamic therapy, are presented. Hybrid hydrogels were synthesized by grafting polyacrylamide onto dextran/dextran sulfate sodium salt using N, N′-methylene-bis-acrylamide as a cross-...

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Main Authors: Oksana Nadtoka, Pavlo Virych, Serhiy Nadtoka, Nataliya Kutsevol
Format: Article
Language:English
Published: Wiley 2020-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2020/6679960
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author Oksana Nadtoka
Pavlo Virych
Serhiy Nadtoka
Nataliya Kutsevol
author_facet Oksana Nadtoka
Pavlo Virych
Serhiy Nadtoka
Nataliya Kutsevol
author_sort Oksana Nadtoka
collection DOAJ
description Development and characterization of hybrid hydrogels loaded with methylene blue, which are designed to apply for photodynamic therapy, are presented. Hybrid hydrogels were synthesized by grafting polyacrylamide onto dextran/dextran sulfate sodium salt using N, N′-methylene-bis-acrylamide as a cross-linker. The differences in microstructure of synthesized hydrogels were proved by scanning electron microscopy. FTIR spectra testify that the chemical nature of hydrogel components affects the hydrogel hydrophilicity. The swelling properties of hydrogels in water and absorption/desorption hydrogels’ ability towards methylene blue were studied. It was shown that dye sorption was dependent on the hydrogel type. The hydrogel based on dextran and polyacrylamide revealed the highest ability to release absorbed dye. The bactericidal effect of this hydrogel loaded with methylene blue and activated by red light in suspension and solid medium of S. aureus was tested. The increase of bactericidal activity of hybrid hydrogel was dependent on radiation doses.
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institution Kabale University
issn 2090-9063
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language English
publishDate 2020-01-01
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spelling doaj-art-3b4dd6d5c84849f7a536d44a4ae175ae2025-08-20T03:55:36ZengWileyJournal of Chemistry2090-90632090-90712020-01-01202010.1155/2020/66799606679960Synthesis and Performance of Hybrid Hydrogels Loaded with Methylene Blue and Its Use for Antimicrobial Photodynamic InactivationOksana Nadtoka0Pavlo Virych1Serhiy Nadtoka2Nataliya Kutsevol3Chemical Department, Taras Shevchenko National University of Kyiv, Kyiv 01601, UkraineChemical Department, Taras Shevchenko National University of Kyiv, Kyiv 01601, UkraineChemical Department, Taras Shevchenko National University of Kyiv, Kyiv 01601, UkraineChemical Department, Taras Shevchenko National University of Kyiv, Kyiv 01601, UkraineDevelopment and characterization of hybrid hydrogels loaded with methylene blue, which are designed to apply for photodynamic therapy, are presented. Hybrid hydrogels were synthesized by grafting polyacrylamide onto dextran/dextran sulfate sodium salt using N, N′-methylene-bis-acrylamide as a cross-linker. The differences in microstructure of synthesized hydrogels were proved by scanning electron microscopy. FTIR spectra testify that the chemical nature of hydrogel components affects the hydrogel hydrophilicity. The swelling properties of hydrogels in water and absorption/desorption hydrogels’ ability towards methylene blue were studied. It was shown that dye sorption was dependent on the hydrogel type. The hydrogel based on dextran and polyacrylamide revealed the highest ability to release absorbed dye. The bactericidal effect of this hydrogel loaded with methylene blue and activated by red light in suspension and solid medium of S. aureus was tested. The increase of bactericidal activity of hybrid hydrogel was dependent on radiation doses.http://dx.doi.org/10.1155/2020/6679960
spellingShingle Oksana Nadtoka
Pavlo Virych
Serhiy Nadtoka
Nataliya Kutsevol
Synthesis and Performance of Hybrid Hydrogels Loaded with Methylene Blue and Its Use for Antimicrobial Photodynamic Inactivation
Journal of Chemistry
title Synthesis and Performance of Hybrid Hydrogels Loaded with Methylene Blue and Its Use for Antimicrobial Photodynamic Inactivation
title_full Synthesis and Performance of Hybrid Hydrogels Loaded with Methylene Blue and Its Use for Antimicrobial Photodynamic Inactivation
title_fullStr Synthesis and Performance of Hybrid Hydrogels Loaded with Methylene Blue and Its Use for Antimicrobial Photodynamic Inactivation
title_full_unstemmed Synthesis and Performance of Hybrid Hydrogels Loaded with Methylene Blue and Its Use for Antimicrobial Photodynamic Inactivation
title_short Synthesis and Performance of Hybrid Hydrogels Loaded with Methylene Blue and Its Use for Antimicrobial Photodynamic Inactivation
title_sort synthesis and performance of hybrid hydrogels loaded with methylene blue and its use for antimicrobial photodynamic inactivation
url http://dx.doi.org/10.1155/2020/6679960
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AT serhiynadtoka synthesisandperformanceofhybridhydrogelsloadedwithmethyleneblueanditsuseforantimicrobialphotodynamicinactivation
AT nataliyakutsevol synthesisandperformanceofhybridhydrogelsloadedwithmethyleneblueanditsuseforantimicrobialphotodynamicinactivation