Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials
Human dental pulp stem cells (HDPSCs) are of special relevance in future regenerative dental therapies. Characterizing cytotoxicity and genotoxicity produced by endodontic materials is required to evaluate the potential for regeneration of injured tissues in future strategies combining regenerative...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/8920356 |
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| author | Alejandro Victoria-Escandell José Santiago Ibañez-Cabellos Sergio Bañuls-Sánchez de Cutanda Ester Berenguer-Pascual Jesús Beltrán-García Eva García-López Federico V. Pallardó José Luis García-Giménez Antonio Pallarés-Sabater Ignacio Zarzosa-López Manuel Monterde |
| author_facet | Alejandro Victoria-Escandell José Santiago Ibañez-Cabellos Sergio Bañuls-Sánchez de Cutanda Ester Berenguer-Pascual Jesús Beltrán-García Eva García-López Federico V. Pallardó José Luis García-Giménez Antonio Pallarés-Sabater Ignacio Zarzosa-López Manuel Monterde |
| author_sort | Alejandro Victoria-Escandell |
| collection | DOAJ |
| description | Human dental pulp stem cells (HDPSCs) are of special relevance in future regenerative dental therapies. Characterizing cytotoxicity and genotoxicity produced by endodontic materials is required to evaluate the potential for regeneration of injured tissues in future strategies combining regenerative and root canal therapies. This study explores the cytotoxicity and genotoxicity mediated by oxidative stress of three endodontic materials that are widely used on HDPSCs: a mineral trioxide aggregate (MTA-Angelus white), an epoxy resin sealant (AH-Plus cement), and an MTA-based cement sealer (MTA-Fillapex). Cell viability and cell death rate were assessed by flow cytometry. Oxidative stress was measured by OxyBlot. Levels of antioxidant enzymes were evaluated by Western blot. Genotoxicity was studied by quantifying the expression levels of DNA damage sensors such as ATM and RAD53 genes and DNA damage repair sensors such as RAD51 and PARP-1. Results indicate that AH-Plus increased apoptosis, oxidative stress, and genotoxicity markers in HDPSCs. MTA-Fillapex was the most cytotoxic oxidative stress inductor and genotoxic material for HDPSCs at longer times in preincubated cell culture medium, and MTA-Angelus was less cytotoxic and genotoxic than AH-Plus and MTA-Fillapex at all times assayed. |
| format | Article |
| id | doaj-art-3b432b81961d4a6890342fdc862fb75c |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-3b432b81961d4a6890342fdc862fb75c2025-02-03T05:57:39ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/89203568920356Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic MaterialsAlejandro Victoria-Escandell0José Santiago Ibañez-Cabellos1Sergio Bañuls-Sánchez de Cutanda2Ester Berenguer-Pascual3Jesús Beltrán-García4Eva García-López5Federico V. Pallardó6José Luis García-Giménez7Antonio Pallarés-Sabater8Ignacio Zarzosa-López9Manuel Monterde10Department of Endodontics, Faculty of Medicine and Dentistry, Catholic University of Valencia “San Vicente Mártir”, C/Quevedo, 2, 46001 Valencia, SpainCenter for Biomedical Network Research on Rare Diseases (CIBERER), CIBER-ISCIII, Valencia, SpainINCLIVA Health Research Institute, Av. de Menéndez y Pelayo 4, 46010 Valencia, SpainDepartment of Physiology, Faculty of Medicine and Dentistry, University of Valencia, Av. Blasco Ibañez 15, 46010 Valencia, SpainDepartment of Physiology, Faculty of Medicine and Dentistry, University of Valencia, Av. Blasco Ibañez 15, 46010 Valencia, SpainDepartment of Physiology, Faculty of Medicine and Dentistry, University of Valencia, Av. Blasco Ibañez 15, 46010 Valencia, SpainCenter for Biomedical Network Research on Rare Diseases (CIBERER), CIBER-ISCIII, Valencia, SpainCenter for Biomedical Network Research on Rare Diseases (CIBERER), CIBER-ISCIII, Valencia, SpainDepartment of Endodontics, Faculty of Medicine and Dentistry, Catholic University of Valencia “San Vicente Mártir”, C/Quevedo, 2, 46001 Valencia, SpainDepartment of Endodontics, Faculty of Medicine and Dentistry, Catholic University of Valencia “San Vicente Mártir”, C/Quevedo, 2, 46001 Valencia, SpainDepartment of Endodontics, Faculty of Medicine and Dentistry, Catholic University of Valencia “San Vicente Mártir”, C/Quevedo, 2, 46001 Valencia, SpainHuman dental pulp stem cells (HDPSCs) are of special relevance in future regenerative dental therapies. Characterizing cytotoxicity and genotoxicity produced by endodontic materials is required to evaluate the potential for regeneration of injured tissues in future strategies combining regenerative and root canal therapies. This study explores the cytotoxicity and genotoxicity mediated by oxidative stress of three endodontic materials that are widely used on HDPSCs: a mineral trioxide aggregate (MTA-Angelus white), an epoxy resin sealant (AH-Plus cement), and an MTA-based cement sealer (MTA-Fillapex). Cell viability and cell death rate were assessed by flow cytometry. Oxidative stress was measured by OxyBlot. Levels of antioxidant enzymes were evaluated by Western blot. Genotoxicity was studied by quantifying the expression levels of DNA damage sensors such as ATM and RAD53 genes and DNA damage repair sensors such as RAD51 and PARP-1. Results indicate that AH-Plus increased apoptosis, oxidative stress, and genotoxicity markers in HDPSCs. MTA-Fillapex was the most cytotoxic oxidative stress inductor and genotoxic material for HDPSCs at longer times in preincubated cell culture medium, and MTA-Angelus was less cytotoxic and genotoxic than AH-Plus and MTA-Fillapex at all times assayed.http://dx.doi.org/10.1155/2017/8920356 |
| spellingShingle | Alejandro Victoria-Escandell José Santiago Ibañez-Cabellos Sergio Bañuls-Sánchez de Cutanda Ester Berenguer-Pascual Jesús Beltrán-García Eva García-López Federico V. Pallardó José Luis García-Giménez Antonio Pallarés-Sabater Ignacio Zarzosa-López Manuel Monterde Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials Stem Cells International |
| title | Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials |
| title_full | Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials |
| title_fullStr | Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials |
| title_full_unstemmed | Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials |
| title_short | Cellular Responses in Human Dental Pulp Stem Cells Treated with Three Endodontic Materials |
| title_sort | cellular responses in human dental pulp stem cells treated with three endodontic materials |
| url | http://dx.doi.org/10.1155/2017/8920356 |
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