Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex
Abstract Membrane protein ComM transiently inhibits cell division during the development of the competence state in the pathogenic bacterium Streptococcus pneumoniae, but the underlying molecular mechanisms remain unclear. Here we show that, in competent cells, ComM moves together with, and reduces...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-60600-z |
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| author | Dimitri Juillot Cyrille Billaudeau Isabelle Mortier-Barrière Aurélien Barbotin Armand Lablaine Patrice Polard Nathalie Campo Rut Carballido-López |
| author_facet | Dimitri Juillot Cyrille Billaudeau Isabelle Mortier-Barrière Aurélien Barbotin Armand Lablaine Patrice Polard Nathalie Campo Rut Carballido-López |
| author_sort | Dimitri Juillot |
| collection | DOAJ |
| description | Abstract Membrane protein ComM transiently inhibits cell division during the development of the competence state in the pathogenic bacterium Streptococcus pneumoniae, but the underlying molecular mechanisms remain unclear. Here we show that, in competent cells, ComM moves together with, and reduces the speed of, septal peptidoglycan synthetic complex FtsW:PBP2x. ComM directly interacts with the putative FtsW:PBP2x activator DivIB, and overproduction of DivIB counteracts FtsW:PBP2x deceleration along the cell division delay in competent cells. Our results support a model in which ComM reduces septal peptidoglycan synthesis by interfering with DivIB activity during competence in S. pneumoniae. |
| format | Article |
| id | doaj-art-3b410ba3ceb64a9686f14ae8e4e82cf1 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-3b410ba3ceb64a9686f14ae8e4e82cf12025-08-20T04:01:35ZengNature PortfolioNature Communications2041-17232025-07-0116111910.1038/s41467-025-60600-zTransient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complexDimitri Juillot0Cyrille Billaudeau1Isabelle Mortier-Barrière2Aurélien Barbotin3Armand Lablaine4Patrice Polard5Nathalie Campo6Rut Carballido-López7Université Paris-Saclay, INRAE, AgroParisTech, Micalis InstituteUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis InstituteLaboratoire de Microbiologie et Génétique Moléculaires, UMR5100, Centre de Biologie Intégrative, Centre Nationale de la Recherche ScientifiqueUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis InstituteUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis InstituteLaboratoire de Microbiologie et Génétique Moléculaires, UMR5100, Centre de Biologie Intégrative, Centre Nationale de la Recherche ScientifiqueLaboratoire de Microbiologie et Génétique Moléculaires, UMR5100, Centre de Biologie Intégrative, Centre Nationale de la Recherche ScientifiqueUniversité Paris-Saclay, INRAE, AgroParisTech, Micalis InstituteAbstract Membrane protein ComM transiently inhibits cell division during the development of the competence state in the pathogenic bacterium Streptococcus pneumoniae, but the underlying molecular mechanisms remain unclear. Here we show that, in competent cells, ComM moves together with, and reduces the speed of, septal peptidoglycan synthetic complex FtsW:PBP2x. ComM directly interacts with the putative FtsW:PBP2x activator DivIB, and overproduction of DivIB counteracts FtsW:PBP2x deceleration along the cell division delay in competent cells. Our results support a model in which ComM reduces septal peptidoglycan synthesis by interfering with DivIB activity during competence in S. pneumoniae.https://doi.org/10.1038/s41467-025-60600-z |
| spellingShingle | Dimitri Juillot Cyrille Billaudeau Isabelle Mortier-Barrière Aurélien Barbotin Armand Lablaine Patrice Polard Nathalie Campo Rut Carballido-López Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex Nature Communications |
| title | Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex |
| title_full | Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex |
| title_fullStr | Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex |
| title_full_unstemmed | Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex |
| title_short | Transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex |
| title_sort | transient inhibition of cell division in competent pneumococcal cells results from deceleration of the septal peptidoglycan complex |
| url | https://doi.org/10.1038/s41467-025-60600-z |
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