Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions
The relationship between hydrogen peroxide and the metabolism of polyamines and the role of NADPH oxidase (NOX) in that relationship under drought conditions remains unclear. To reveal the relationship, expression levels of the genes in polyamine metabolism, such as arginine decarboxylase, agmatine...
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| Format: | Article |
| Language: | English |
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Ankara University
2022-10-01
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| Series: | Journal of Agricultural Sciences |
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| Online Access: | https://dergipark.org.tr/tr/download/article-file/1509434 |
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| author | Aykut Sağlam Mehmet Demiralay Fuat Yetişsin Asım Kadıoğlu |
| author_facet | Aykut Sağlam Mehmet Demiralay Fuat Yetişsin Asım Kadıoğlu |
| author_sort | Aykut Sağlam |
| collection | DOAJ |
| description | The relationship between hydrogen peroxide and the metabolism of polyamines and the role of NADPH oxidase (NOX) in that relationship under drought conditions remains unclear. To reveal the relationship, expression levels of the genes in polyamine metabolism, such as arginine decarboxylase, agmatine aminohydrolase, spermidine synthase, S-adenosyl methionine decarboxylase, diamine oxidase, and polyamine oxidase were determined by RT PCR under drought stress combined with exogenous hydrogen peroxide (H2O2) and diphenyleneiodonium chloride (DPI) treatments in maize seedlings. In addition, some basic stress parameters (leaf water potential, lipid peroxidation), levels of polyamines (putrescine, spermidine, and spermine), and gene expression of NOX were measured under drought stress. Exogenous H2O2 induced the polyamine content by up-regulating polyamine-synthesizing genes and downregulating polyamine oxidizing genes. When the NOX enzyme was inhibited by DPI, the polyamine pathway tended towards degradation instead of production. Exogenous H2O2 regulated the metabolism of polyamines to promote their synthesis, and NOX played a key role in that regulation. |
| format | Article |
| id | doaj-art-3b3d09cae70a418597f80c024e5175ed |
| institution | OA Journals |
| issn | 1300-7580 2148-9297 |
| language | English |
| publishDate | 2022-10-01 |
| publisher | Ankara University |
| record_format | Article |
| series | Journal of Agricultural Sciences |
| spelling | doaj-art-3b3d09cae70a418597f80c024e5175ed2025-08-20T01:56:34ZengAnkara UniversityJournal of Agricultural Sciences1300-75802148-92972022-10-0128461362510.15832/ankutbd.86100845Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress ConditionsAykut Sağlam0Mehmet Demiralay1Fuat Yetişsin2Asım Kadıoğlu3KARADENİZ TEKNİK ÜNİVERSİTESİARTVİN ÇORUH ÜNİVERSİTESİMUŞ ALPARSLAN ÜNİVERSİTESİKARADENİZ TEKNİK ÜNİVERSİTESİThe relationship between hydrogen peroxide and the metabolism of polyamines and the role of NADPH oxidase (NOX) in that relationship under drought conditions remains unclear. To reveal the relationship, expression levels of the genes in polyamine metabolism, such as arginine decarboxylase, agmatine aminohydrolase, spermidine synthase, S-adenosyl methionine decarboxylase, diamine oxidase, and polyamine oxidase were determined by RT PCR under drought stress combined with exogenous hydrogen peroxide (H2O2) and diphenyleneiodonium chloride (DPI) treatments in maize seedlings. In addition, some basic stress parameters (leaf water potential, lipid peroxidation), levels of polyamines (putrescine, spermidine, and spermine), and gene expression of NOX were measured under drought stress. Exogenous H2O2 induced the polyamine content by up-regulating polyamine-synthesizing genes and downregulating polyamine oxidizing genes. When the NOX enzyme was inhibited by DPI, the polyamine pathway tended towards degradation instead of production. Exogenous H2O2 regulated the metabolism of polyamines to promote their synthesis, and NOX played a key role in that regulation.https://dergipark.org.tr/tr/download/article-file/1509434antioxidant enzymesarginine decarboxylasediphenyleneiodonium chloridepolyamine oxidationreactive oxygen species |
| spellingShingle | Aykut Sağlam Mehmet Demiralay Fuat Yetişsin Asım Kadıoğlu Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions Journal of Agricultural Sciences antioxidant enzymes arginine decarboxylase diphenyleneiodonium chloride polyamine oxidation reactive oxygen species |
| title | Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions |
| title_full | Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions |
| title_fullStr | Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions |
| title_full_unstemmed | Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions |
| title_short | Investigation of The Roles of Hydrogen Peroxide and NADPH Oxidase in The Regulation of Polyamine Metabolism in Maize Plants under Drought Stress Conditions |
| title_sort | investigation of the roles of hydrogen peroxide and nadph oxidase in the regulation of polyamine metabolism in maize plants under drought stress conditions |
| topic | antioxidant enzymes arginine decarboxylase diphenyleneiodonium chloride polyamine oxidation reactive oxygen species |
| url | https://dergipark.org.tr/tr/download/article-file/1509434 |
| work_keys_str_mv | AT aykutsaglam investigationoftherolesofhydrogenperoxideandnadphoxidaseintheregulationofpolyaminemetabolisminmaizeplantsunderdroughtstressconditions AT mehmetdemiralay investigationoftherolesofhydrogenperoxideandnadphoxidaseintheregulationofpolyaminemetabolisminmaizeplantsunderdroughtstressconditions AT fuatyetissin investigationoftherolesofhydrogenperoxideandnadphoxidaseintheregulationofpolyaminemetabolisminmaizeplantsunderdroughtstressconditions AT asımkadıoglu investigationoftherolesofhydrogenperoxideandnadphoxidaseintheregulationofpolyaminemetabolisminmaizeplantsunderdroughtstressconditions |