Molecular evidence for the efficacy of PBM therapy in the treatment of oral lichen planus
Purpose: Oral lichen planus (OLP) is a chronic inflammatory disease, in which T-Lymphocytes induce apoptosis of basal keratinocytes, leading to the formation of symptomatic lesions. It is assumed that blocking the cell death program and enhancing cell proliferation would be crucial to the healing pr...
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| Format: | Article |
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Elsevier
2025-02-01
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| Series: | Photodiagnosis and Photodynamic Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1572100025000092 |
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| author | Maria Zaharieva Mutafchieva Milena Nenkova Draganova Plamen Ivanov Zagorchev Reem Hanna Georgi Tomchev Tomov |
| author_facet | Maria Zaharieva Mutafchieva Milena Nenkova Draganova Plamen Ivanov Zagorchev Reem Hanna Georgi Tomchev Tomov |
| author_sort | Maria Zaharieva Mutafchieva |
| collection | DOAJ |
| description | Purpose: Oral lichen planus (OLP) is a chronic inflammatory disease, in which T-Lymphocytes induce apoptosis of basal keratinocytes, leading to the formation of symptomatic lesions. It is assumed that blocking the cell death program and enhancing cell proliferation would be crucial to the healing process. The aim of the study was to verify the efficacy of Photobiomodulation (PBM) in OLP management, by evaluating the effects of laser irradiation on the processes of apoptosis and cell proliferation. Methods: Twenty patients with OLP underwent PBM with 810 nm diode laser (0.5 W, 30 s, 1.2 J/cm2), 3 times a week for one month. The size and clinical scores of the lesions and pain level were recorded and compared before and after therapy using Thongprasom sign scoring system and Visual Analogue Scale, respectively. Biopsies were taken before and after therapy and analyzed immunohistochemically for bcl-2 and Ki-67 expression. Tissue sections from 10 healthy volunteers were used as controls for the levels of these markers in normal oral mucosa. Results: PBM significantly reduced patients’ pain intesity and improved the clinical signs of the lesions. OLP tissue sections demonstrated lower staining for bcl-2 and Ki-67 compared to healthy controls. After therapy the expression of bcl-2 increased significantly. An enhance in Ki-67 immunoreactivity was revealed predominantly in the atrophic-erosive epithelium. Conclusion: PBM is an effective treatment modality for OLP patients. Erosive forms of the disease responded better to laser irradiation than keratotic subtypes. At the molecular level, PBM is likely to recover the impaired cell cycle mechanisms and keratinocyte proliferation. |
| format | Article |
| id | doaj-art-3b2ec0789c31476a98765f3a769c894d |
| institution | Kabale University |
| issn | 1572-1000 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photodiagnosis and Photodynamic Therapy |
| spelling | doaj-art-3b2ec0789c31476a98765f3a769c894d2025-02-01T04:11:50ZengElsevierPhotodiagnosis and Photodynamic Therapy1572-10002025-02-0151104479Molecular evidence for the efficacy of PBM therapy in the treatment of oral lichen planusMaria Zaharieva Mutafchieva0Milena Nenkova Draganova1Plamen Ivanov Zagorchev2Reem Hanna3Georgi Tomchev Tomov4Department of Periodontology and Oral Mucosa Diseases, Faculty of Dental Medicine, Medical University of Plovdiv, Plovdiv, 4000, Bulgaria; Corresponding author.Department of Medical Biology, Faculty of Medicine, Medical University of Plovdiv, Plovdiv, 4000, Bulgaria; Research Institute, Medical University of Plovdiv, Plovdiv, 4000, BulgariaResearch Institute, Medical University of Plovdiv, Plovdiv, 4000, Bulgaria; Department of Medical Physics and Biophysics, Faculty of Pharmacy, Medical University-Plovdiv, Plovdiv, 4000, BulgariaDepartment of Restorative Dental Sciences, UCL-Eastman Dental Institute, Medical Faculty, University College London, London, UK; Department of Oral Surgery, King's College Hospital NHS Foundation Trust, London, UK; Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, Genoa, ItalyDepartment of Healthcare and Social Work, New Bulgarian University, Sofia, BulgariaPurpose: Oral lichen planus (OLP) is a chronic inflammatory disease, in which T-Lymphocytes induce apoptosis of basal keratinocytes, leading to the formation of symptomatic lesions. It is assumed that blocking the cell death program and enhancing cell proliferation would be crucial to the healing process. The aim of the study was to verify the efficacy of Photobiomodulation (PBM) in OLP management, by evaluating the effects of laser irradiation on the processes of apoptosis and cell proliferation. Methods: Twenty patients with OLP underwent PBM with 810 nm diode laser (0.5 W, 30 s, 1.2 J/cm2), 3 times a week for one month. The size and clinical scores of the lesions and pain level were recorded and compared before and after therapy using Thongprasom sign scoring system and Visual Analogue Scale, respectively. Biopsies were taken before and after therapy and analyzed immunohistochemically for bcl-2 and Ki-67 expression. Tissue sections from 10 healthy volunteers were used as controls for the levels of these markers in normal oral mucosa. Results: PBM significantly reduced patients’ pain intesity and improved the clinical signs of the lesions. OLP tissue sections demonstrated lower staining for bcl-2 and Ki-67 compared to healthy controls. After therapy the expression of bcl-2 increased significantly. An enhance in Ki-67 immunoreactivity was revealed predominantly in the atrophic-erosive epithelium. Conclusion: PBM is an effective treatment modality for OLP patients. Erosive forms of the disease responded better to laser irradiation than keratotic subtypes. At the molecular level, PBM is likely to recover the impaired cell cycle mechanisms and keratinocyte proliferation.http://www.sciencedirect.com/science/article/pii/S1572100025000092810 nm diode laserBcl-2 and Ki-67Oral lichen planusPhotobiomodulationPBMOLP |
| spellingShingle | Maria Zaharieva Mutafchieva Milena Nenkova Draganova Plamen Ivanov Zagorchev Reem Hanna Georgi Tomchev Tomov Molecular evidence for the efficacy of PBM therapy in the treatment of oral lichen planus Photodiagnosis and Photodynamic Therapy 810 nm diode laser Bcl-2 and Ki-67 Oral lichen planus Photobiomodulation PBM OLP |
| title | Molecular evidence for the efficacy of PBM therapy in the treatment of oral lichen planus |
| title_full | Molecular evidence for the efficacy of PBM therapy in the treatment of oral lichen planus |
| title_fullStr | Molecular evidence for the efficacy of PBM therapy in the treatment of oral lichen planus |
| title_full_unstemmed | Molecular evidence for the efficacy of PBM therapy in the treatment of oral lichen planus |
| title_short | Molecular evidence for the efficacy of PBM therapy in the treatment of oral lichen planus |
| title_sort | molecular evidence for the efficacy of pbm therapy in the treatment of oral lichen planus |
| topic | 810 nm diode laser Bcl-2 and Ki-67 Oral lichen planus Photobiomodulation PBM OLP |
| url | http://www.sciencedirect.com/science/article/pii/S1572100025000092 |
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