Expression and Significances of Contactin-1 in Human Gastric Cancer
Background. This study aimed at determining the relationship between vascular endothelial growth factor-C (VEGF-C), vascular endothelial growth factor receptor-3 (VEGFR-3), and contactin-1 (CNTN-1) expression in gastric cancer (GC). Methods. The expression level of CNTN-1 mRNA and...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2013/210205 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832552284703162368 |
|---|---|
| author | Ji-Wei Yu Sheng-Hua Wu Rui-qi Lu Ju-gang Wu Xiao-Chun Ni Gou-cai Zhou Hai-guang Jiang Lin-Hai Zheng Xiao-Qiang Li Guang-Ye Du Bo-Jian Jiang |
| author_facet | Ji-Wei Yu Sheng-Hua Wu Rui-qi Lu Ju-gang Wu Xiao-Chun Ni Gou-cai Zhou Hai-guang Jiang Lin-Hai Zheng Xiao-Qiang Li Guang-Ye Du Bo-Jian Jiang |
| author_sort | Ji-Wei Yu |
| collection | DOAJ |
| description | Background. This study aimed at determining the relationship between vascular endothelial growth factor-C (VEGF-C), vascular endothelial growth factor
receptor-3 (VEGFR-3), and contactin-1 (CNTN-1) expression in gastric cancer (GC). Methods. The expression level of CNTN-1 mRNA and CNTN-1 protein of 33 cases was
determined using RT-PCR and Western Blot. And 105 cases were immunohistochemically examined for VEGF-C, VEGFR-3, and CNTN-1 expressions.
Assessment of lymphatic vessel density (LVD) was also performed by D2-40 immunostaining. Then we analyzed the relationships between VEGF-C, VEGFR-3,
and CNTN-1, as well as their correlations with clinicopathologic features, LVD, and survival time. Results. The positivity rate of VEGF-C, VEGFR-3, and CNTN-1
in primary tumor was 56.19%, 64.76%, and 58.09%. The expression of CNTN-1 significantly correlated with VEGF-C
(P<0.001) and VEGFR-3
(P<0.001). All of them were closely related to TNM stage, lymphatic
invasion, and lymph node involvement (P<0.05). LVD was significantly
correlated with VEGF-C (P=0.001), VEGFR-3
(P=0.011), and CNTN-1 expression
(P<0.001). VEGF-C, VEGFR-3, and CNTN-1 expression significantly
associated with poorer prognosis (P<0.001,
P=0.034,
P=0.012, resp.). Conclusion. CNTN-1 associated with
VEGF-C and VEGFR-3 expression in GC. All of them correlated with lymphatic metastasis, which might play an important role in the lymphatic invasion via lymphangiogenesis pathway in GC. |
| format | Article |
| id | doaj-art-3b2d6b2cbe914dc1882856977c148134 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-3b2d6b2cbe914dc1882856977c1481342025-02-03T05:59:04ZengWileyGastroenterology Research and Practice1687-61211687-630X2013-01-01201310.1155/2013/210205210205Expression and Significances of Contactin-1 in Human Gastric CancerJi-Wei Yu0Sheng-Hua Wu1Rui-qi Lu2Ju-gang Wu3Xiao-Chun Ni4Gou-cai Zhou5Hai-guang Jiang6Lin-Hai Zheng7Xiao-Qiang Li8Guang-Ye Du9Bo-Jian Jiang10Department of General Surgery, Shanghai 3rd People's Hospital, School of Medicine, Shanghai Jiao-tong University, Shanghai 201900, ChinaDepartment of General Surgery, Shanghai 3rd People's Hospital, School of Medicine, Shanghai Jiao-tong University, Shanghai 201900, ChinaDepartment of General Surgery, Shanghai 3rd People's Hospital, School of Medicine, Shanghai Jiao-tong University, Shanghai 201900, ChinaDepartment of General Surgery, Shanghai 3rd People's Hospital, School of Medicine, Shanghai Jiao-tong University, Shanghai 201900, ChinaDepartment of General Surgery, Shanghai 3rd People's Hospital, School of Medicine, Shanghai Jiao-tong University, Shanghai 201900, ChinaDepartment of General Surgery, Shanghai 3rd People's Hospital, School of Medicine, Shanghai Jiao-tong University, Shanghai 201900, ChinaDepartment of General Surgery, Shanghai 3rd People's Hospital, School of Medicine, Shanghai Jiao-tong University, Shanghai 201900, ChinaDepartment of General Surgery, Shanghai 3rd People's Hospital, School of Medicine, Shanghai Jiao-tong University, Shanghai 201900, ChinaDepartment of Pathology, Shanghai 3rd People's Hospital, School of Medicine, Shanghai Jiao-tong University, Shanghai 201900, ChinaDepartment of Pathology, Shanghai 3rd People's Hospital, School of Medicine, Shanghai Jiao-tong University, Shanghai 201900, ChinaDepartment of General Surgery, Shanghai 3rd People's Hospital, School of Medicine, Shanghai Jiao-tong University, Shanghai 201900, ChinaBackground. This study aimed at determining the relationship between vascular endothelial growth factor-C (VEGF-C), vascular endothelial growth factor receptor-3 (VEGFR-3), and contactin-1 (CNTN-1) expression in gastric cancer (GC). Methods. The expression level of CNTN-1 mRNA and CNTN-1 protein of 33 cases was determined using RT-PCR and Western Blot. And 105 cases were immunohistochemically examined for VEGF-C, VEGFR-3, and CNTN-1 expressions. Assessment of lymphatic vessel density (LVD) was also performed by D2-40 immunostaining. Then we analyzed the relationships between VEGF-C, VEGFR-3, and CNTN-1, as well as their correlations with clinicopathologic features, LVD, and survival time. Results. The positivity rate of VEGF-C, VEGFR-3, and CNTN-1 in primary tumor was 56.19%, 64.76%, and 58.09%. The expression of CNTN-1 significantly correlated with VEGF-C (P<0.001) and VEGFR-3 (P<0.001). All of them were closely related to TNM stage, lymphatic invasion, and lymph node involvement (P<0.05). LVD was significantly correlated with VEGF-C (P=0.001), VEGFR-3 (P=0.011), and CNTN-1 expression (P<0.001). VEGF-C, VEGFR-3, and CNTN-1 expression significantly associated with poorer prognosis (P<0.001, P=0.034, P=0.012, resp.). Conclusion. CNTN-1 associated with VEGF-C and VEGFR-3 expression in GC. All of them correlated with lymphatic metastasis, which might play an important role in the lymphatic invasion via lymphangiogenesis pathway in GC.http://dx.doi.org/10.1155/2013/210205 |
| spellingShingle | Ji-Wei Yu Sheng-Hua Wu Rui-qi Lu Ju-gang Wu Xiao-Chun Ni Gou-cai Zhou Hai-guang Jiang Lin-Hai Zheng Xiao-Qiang Li Guang-Ye Du Bo-Jian Jiang Expression and Significances of Contactin-1 in Human Gastric Cancer Gastroenterology Research and Practice |
| title | Expression and Significances of Contactin-1 in Human Gastric Cancer |
| title_full | Expression and Significances of Contactin-1 in Human Gastric Cancer |
| title_fullStr | Expression and Significances of Contactin-1 in Human Gastric Cancer |
| title_full_unstemmed | Expression and Significances of Contactin-1 in Human Gastric Cancer |
| title_short | Expression and Significances of Contactin-1 in Human Gastric Cancer |
| title_sort | expression and significances of contactin 1 in human gastric cancer |
| url | http://dx.doi.org/10.1155/2013/210205 |
| work_keys_str_mv | AT jiweiyu expressionandsignificancesofcontactin1inhumangastriccancer AT shenghuawu expressionandsignificancesofcontactin1inhumangastriccancer AT ruiqilu expressionandsignificancesofcontactin1inhumangastriccancer AT jugangwu expressionandsignificancesofcontactin1inhumangastriccancer AT xiaochunni expressionandsignificancesofcontactin1inhumangastriccancer AT goucaizhou expressionandsignificancesofcontactin1inhumangastriccancer AT haiguangjiang expressionandsignificancesofcontactin1inhumangastriccancer AT linhaizheng expressionandsignificancesofcontactin1inhumangastriccancer AT xiaoqiangli expressionandsignificancesofcontactin1inhumangastriccancer AT guangyedu expressionandsignificancesofcontactin1inhumangastriccancer AT bojianjiang expressionandsignificancesofcontactin1inhumangastriccancer |