TRAF1 Gene Polymorphism Correlates with the Titre of Gp210 Antibody in Patients with Primary Biliary Cirrhosis
Background. Polymorphisms of TRAF1 (Tumor necrosis factor receptor-associated factor 1) are associated with rheumatoid arthritis (RA). Whether TRAF1 polymorphisms confer increased risk for primary biliary cirrhosis (PBC), an autoimmune liver disease which can co-exist with RA, is unknown. Aim of...
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/487521 |
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| author | Agnieszka Kempinska-Podhorodecka Zakera Shums Michał Wasilewicz Ewa Wunsch Malgorzata Milkiewicz Dimitrios P. Bogdanos Gary L. Norman Piotr Milkiewicz |
| author_facet | Agnieszka Kempinska-Podhorodecka Zakera Shums Michał Wasilewicz Ewa Wunsch Malgorzata Milkiewicz Dimitrios P. Bogdanos Gary L. Norman Piotr Milkiewicz |
| author_sort | Agnieszka Kempinska-Podhorodecka |
| collection | DOAJ |
| description | Background. Polymorphisms of TRAF1 (Tumor necrosis factor receptor-associated factor 1) are associated with rheumatoid arthritis (RA). Whether TRAF1 polymorphisms confer increased risk for primary biliary cirrhosis (PBC), an autoimmune liver disease which can co-exist with RA, is unknown.
Aim of the Study. To assess the frequency of the RA-conferring susceptibility TRAF1 polymorphisms rs3761847 and rs2900180 in a cohort of PBC patients. The association of TRAF1 polymorphisms with clinical features and autoantibody markers was also analyzed.
Methods. We studied 179 PBC patients and 300 controls. Samples were genotyped for TRAF1 gene polymorphisms by real-time PCR. Autoantibodies were tested by ELISA.
Results. The frequency of rs3761847 and rs2900180 polymorphisms did not differ between patients and controls. Laboratory or clinical features were not associated with specific polymorphisms. Gp210 autoantibody titres were conspicuously higher among GG homozygotes of rs3761847 as compared with AA homozygotes (P=0.02). In contrast, antichromatin titers were higher in AA compared to GG rs3761847 homozygotes (P=0.04). Rheumatoid factor IgG titres were significantly higher in rs2900180 TT homozygotes than CC homozygotes (P=0.02).
Conclusions. TRAF1 polymorphisms occur with the similar frequency in PBC patients and in the general population, but their presence is probably involved in the regulation of specific PBC-related autoantibodies. |
| format | Article |
| id | doaj-art-3b2a3f08c3594b909407561c94693755 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-3b2a3f08c3594b909407561c946937552025-02-03T01:21:25ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/487521487521TRAF1 Gene Polymorphism Correlates with the Titre of Gp210 Antibody in Patients with Primary Biliary CirrhosisAgnieszka Kempinska-Podhorodecka0Zakera Shums1Michał Wasilewicz2Ewa Wunsch3Malgorzata Milkiewicz4Dimitrios P. Bogdanos5Gary L. Norman6Piotr Milkiewicz7Medical Biology Laboratory, Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, 70-111 Szczecin, PolandINOVA Diagnostics, San Diego, CA 92131-1638, USALiver Unit, Pomeranian Medical University, 70-111 Szczecin, PolandLiver Unit, Pomeranian Medical University, 70-111 Szczecin, PolandMedical Biology Laboratory, Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, 70-111 Szczecin, PolandInstitute of Liver Studies, School of Medicine, King's College London, London SE5 9RS, UKINOVA Diagnostics, San Diego, CA 92131-1638, USALiver Unit, Pomeranian Medical University, 70-111 Szczecin, PolandBackground. Polymorphisms of TRAF1 (Tumor necrosis factor receptor-associated factor 1) are associated with rheumatoid arthritis (RA). Whether TRAF1 polymorphisms confer increased risk for primary biliary cirrhosis (PBC), an autoimmune liver disease which can co-exist with RA, is unknown. Aim of the Study. To assess the frequency of the RA-conferring susceptibility TRAF1 polymorphisms rs3761847 and rs2900180 in a cohort of PBC patients. The association of TRAF1 polymorphisms with clinical features and autoantibody markers was also analyzed. Methods. We studied 179 PBC patients and 300 controls. Samples were genotyped for TRAF1 gene polymorphisms by real-time PCR. Autoantibodies were tested by ELISA. Results. The frequency of rs3761847 and rs2900180 polymorphisms did not differ between patients and controls. Laboratory or clinical features were not associated with specific polymorphisms. Gp210 autoantibody titres were conspicuously higher among GG homozygotes of rs3761847 as compared with AA homozygotes (P=0.02). In contrast, antichromatin titers were higher in AA compared to GG rs3761847 homozygotes (P=0.04). Rheumatoid factor IgG titres were significantly higher in rs2900180 TT homozygotes than CC homozygotes (P=0.02). Conclusions. TRAF1 polymorphisms occur with the similar frequency in PBC patients and in the general population, but their presence is probably involved in the regulation of specific PBC-related autoantibodies.http://dx.doi.org/10.1155/2012/487521 |
| spellingShingle | Agnieszka Kempinska-Podhorodecka Zakera Shums Michał Wasilewicz Ewa Wunsch Malgorzata Milkiewicz Dimitrios P. Bogdanos Gary L. Norman Piotr Milkiewicz TRAF1 Gene Polymorphism Correlates with the Titre of Gp210 Antibody in Patients with Primary Biliary Cirrhosis Clinical and Developmental Immunology |
| title | TRAF1 Gene Polymorphism Correlates with the Titre of Gp210 Antibody in Patients with Primary Biliary Cirrhosis |
| title_full | TRAF1 Gene Polymorphism Correlates with the Titre of Gp210 Antibody in Patients with Primary Biliary Cirrhosis |
| title_fullStr | TRAF1 Gene Polymorphism Correlates with the Titre of Gp210 Antibody in Patients with Primary Biliary Cirrhosis |
| title_full_unstemmed | TRAF1 Gene Polymorphism Correlates with the Titre of Gp210 Antibody in Patients with Primary Biliary Cirrhosis |
| title_short | TRAF1 Gene Polymorphism Correlates with the Titre of Gp210 Antibody in Patients with Primary Biliary Cirrhosis |
| title_sort | traf1 gene polymorphism correlates with the titre of gp210 antibody in patients with primary biliary cirrhosis |
| url | http://dx.doi.org/10.1155/2012/487521 |
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