Acetamiprid-induced testicular toxicity in mice: ameliorative effect and potential mechanisms of morin
Abstract Background Acetamiprid (ACP) is a novel chloronicotinyl insecticide that has been extensively utilized in agricultural, domestic, and public health contexts for nearly two decades. However, its potential to induce organ damage, including reproductive toxicity in mammals, has emerged as a si...
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BMC
2025-06-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-025-04944-5 |
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| author | Nihal Turkmen Alemdar Selim Demir Esin Yulug Ali Kulaber Elif Ayazoglu Demir Nadire Sevdenur Erdogan Ahmet Mentese Yuksel Aliyazicioglu |
| author_facet | Nihal Turkmen Alemdar Selim Demir Esin Yulug Ali Kulaber Elif Ayazoglu Demir Nadire Sevdenur Erdogan Ahmet Mentese Yuksel Aliyazicioglu |
| author_sort | Nihal Turkmen Alemdar |
| collection | DOAJ |
| description | Abstract Background Acetamiprid (ACP) is a novel chloronicotinyl insecticide that has been extensively utilized in agricultural, domestic, and public health contexts for nearly two decades. However, its potential to induce organ damage, including reproductive toxicity in mammals, has emerged as a significant concern. Morin is a naturally occurring flavonol that has gained prominence as a food supplement in recent years due to its antioxidant and anti-inflammatory properties. The objective of this study was to evaluate the protective effect of morin against testicular damage in mice subjected to ACP exposure. Methods Thirty male Balb/c mice were randomly assigned to one of five groups, with the following treatment allocations: control, ACP (20 mg/kg), ACP + morin (15 and 30 mg/kg), and only morin (30 mg/kg). ACP and morin applications were conducted orally over a period of 14 days. Hormonal analyses were conducted on serum samples obtained from the mice, while biochemical and histological evaluations were performed on testicular samples. Results The biochemical results demonstrated that ACP elevated oxidative stress, inflammation, and ER stress in testicular tissue by inhibiting the Nrf2 pathway, a finding that was corroborated by histopathological analyses. However, morin treatments eliminated ACP-induced Nrf2 inhibition and to activate antioxidant and anti-inflammatory mechanisms. These findings were also corroborated by the restoration of serum testosterone and inhibin B levels and the diminution of histopathological lesions. Conclusions Overall, the findings indicated that morin may have potential protective properties against ACP-associated reproductive toxicity, however, further research is required to determine the detailed molecular mechanisms. |
| format | Article |
| id | doaj-art-3b2252a110a8473bb024f74d17d0ba51 |
| institution | OA Journals |
| issn | 2662-7671 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj-art-3b2252a110a8473bb024f74d17d0ba512025-08-20T02:31:03ZengBMCBMC Complementary Medicine and Therapies2662-76712025-06-0125111210.1186/s12906-025-04944-5Acetamiprid-induced testicular toxicity in mice: ameliorative effect and potential mechanisms of morinNihal Turkmen Alemdar0Selim Demir1Esin Yulug2Ali Kulaber3Elif Ayazoglu Demir4Nadire Sevdenur Erdogan5Ahmet Mentese6Yuksel Aliyazicioglu7Department of Medical Services and Techniques, Vocational School of Health Services, Recep Tayyip Erdogan UniversityDepartment of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical UniversityDepartment of Histology and Embryology, Faculty of Medicine, Karadeniz Technical UniversityDepartment of Histology and Embryology, Faculty of Medicine, Karadeniz Technical UniversityDepartment of Chemistry and Chemical Processing Technologies, Macka Vocational School, Karadeniz Technical UniversityDepartment of Histology and Embryology, Faculty of Medicine, Karadeniz Technical UniversityDepartment of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical UniversityDepartment of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical UniversityAbstract Background Acetamiprid (ACP) is a novel chloronicotinyl insecticide that has been extensively utilized in agricultural, domestic, and public health contexts for nearly two decades. However, its potential to induce organ damage, including reproductive toxicity in mammals, has emerged as a significant concern. Morin is a naturally occurring flavonol that has gained prominence as a food supplement in recent years due to its antioxidant and anti-inflammatory properties. The objective of this study was to evaluate the protective effect of morin against testicular damage in mice subjected to ACP exposure. Methods Thirty male Balb/c mice were randomly assigned to one of five groups, with the following treatment allocations: control, ACP (20 mg/kg), ACP + morin (15 and 30 mg/kg), and only morin (30 mg/kg). ACP and morin applications were conducted orally over a period of 14 days. Hormonal analyses were conducted on serum samples obtained from the mice, while biochemical and histological evaluations were performed on testicular samples. Results The biochemical results demonstrated that ACP elevated oxidative stress, inflammation, and ER stress in testicular tissue by inhibiting the Nrf2 pathway, a finding that was corroborated by histopathological analyses. However, morin treatments eliminated ACP-induced Nrf2 inhibition and to activate antioxidant and anti-inflammatory mechanisms. These findings were also corroborated by the restoration of serum testosterone and inhibin B levels and the diminution of histopathological lesions. Conclusions Overall, the findings indicated that morin may have potential protective properties against ACP-associated reproductive toxicity, however, further research is required to determine the detailed molecular mechanisms.https://doi.org/10.1186/s12906-025-04944-5AcetamipridER stressInflammationNrf2Oxidative stressTesticular toxicity |
| spellingShingle | Nihal Turkmen Alemdar Selim Demir Esin Yulug Ali Kulaber Elif Ayazoglu Demir Nadire Sevdenur Erdogan Ahmet Mentese Yuksel Aliyazicioglu Acetamiprid-induced testicular toxicity in mice: ameliorative effect and potential mechanisms of morin BMC Complementary Medicine and Therapies Acetamiprid ER stress Inflammation Nrf2 Oxidative stress Testicular toxicity |
| title | Acetamiprid-induced testicular toxicity in mice: ameliorative effect and potential mechanisms of morin |
| title_full | Acetamiprid-induced testicular toxicity in mice: ameliorative effect and potential mechanisms of morin |
| title_fullStr | Acetamiprid-induced testicular toxicity in mice: ameliorative effect and potential mechanisms of morin |
| title_full_unstemmed | Acetamiprid-induced testicular toxicity in mice: ameliorative effect and potential mechanisms of morin |
| title_short | Acetamiprid-induced testicular toxicity in mice: ameliorative effect and potential mechanisms of morin |
| title_sort | acetamiprid induced testicular toxicity in mice ameliorative effect and potential mechanisms of morin |
| topic | Acetamiprid ER stress Inflammation Nrf2 Oxidative stress Testicular toxicity |
| url | https://doi.org/10.1186/s12906-025-04944-5 |
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