Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung Inflammation
Inflammation is an essential process in many pulmonary diseases in which kinins are generated by protease action on kininogen, a phenomenon that is blocked by protease inhibitors. We evaluated kinin release in an in vivo lung inflammation model in rats, in the presence or absence of CeKI (C. echinat...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Pulmonary Medicine |
| Online Access: | http://dx.doi.org/10.1155/2016/9425807 |
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| author | Ilana Cruz-Silva Viviane Abreu Nunes Andrezza Justino Gozzo Priscila Praxedes-Garcia Aparecida Sadae Tanaka Kazuaki Shimamoto Mariana Silva Araujo |
| author_facet | Ilana Cruz-Silva Viviane Abreu Nunes Andrezza Justino Gozzo Priscila Praxedes-Garcia Aparecida Sadae Tanaka Kazuaki Shimamoto Mariana Silva Araujo |
| author_sort | Ilana Cruz-Silva |
| collection | DOAJ |
| description | Inflammation is an essential process in many pulmonary diseases in which kinins are generated by protease action on kininogen, a phenomenon that is blocked by protease inhibitors. We evaluated kinin release in an in vivo lung inflammation model in rats, in the presence or absence of CeKI (C. echinata kallikrein inhibitor), a plasma kallikrein, cathepsin G, and proteinase-3 inhibitor, and rCeEI (recombinant C. echinata elastase inhibitor), which inhibits these proteases and also neutrophil elastase. Wistar rats were intravenously treated with buffer (negative control) or inhibitors and, subsequently, lipopolysaccharide was injected into their lungs. Blood, bronchoalveolar lavage fluid (BALF), and lung tissue were collected. In plasma, kinin release was higher in the LPS-treated animals in comparison to CeKI or rCeEI groups. rCeEI-treated animals presented less kinin than CeKI-treated group. Our data suggest that kinins play a pivotal role in lung inflammation and may be generated by different enzymes; however, neutrophil elastase seems to be the most important in the lung tissue context. These results open perspectives for a better understanding of biological process where neutrophil enzymes participate and indicate these plant inhibitors and their recombinant correlates for therapeutic trials involving pulmonary diseases. |
| format | Article |
| id | doaj-art-3b0fbb1dc5e64f8e88b29d5ffc05bfed |
| institution | OA Journals |
| issn | 2090-1836 2090-1844 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pulmonary Medicine |
| spelling | doaj-art-3b0fbb1dc5e64f8e88b29d5ffc05bfed2025-08-20T02:08:03ZengWileyPulmonary Medicine2090-18362090-18442016-01-01201610.1155/2016/94258079425807Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung InflammationIlana Cruz-Silva0Viviane Abreu Nunes1Andrezza Justino Gozzo2Priscila Praxedes-Garcia3Aparecida Sadae Tanaka4Kazuaki Shimamoto5Mariana Silva Araujo6Department of Biochemistry, Universidade Federal de São Paulo, Rua Três de Maio, No. 100, 04044-020 São Paulo, SP, BrazilSchool of Arts, Sciences and Humanities, Universidade de São Paulo, Avenida Arlindo Bettio, No. 1000, 03828-000 São Paulo, SP, BrazilDepartment of Marine Sciences, Universidade Federal de São Paulo, Rua Doutor Carvalho de Mendonça, No. 144, 11070-100 Santos, SP, BrazilDepartment of Biochemistry, Universidade Federal de São Paulo, Rua Três de Maio, No. 100, 04044-020 São Paulo, SP, BrazilDepartment of Biochemistry, Universidade Federal de São Paulo, Rua Três de Maio, No. 100, 04044-020 São Paulo, SP, BrazilJapan Health Care College, Sinei 434-1, Kiyota-ku, Sapporo, JapanDepartment of Biochemistry, Universidade Federal de São Paulo, Rua Três de Maio, No. 100, 04044-020 São Paulo, SP, BrazilInflammation is an essential process in many pulmonary diseases in which kinins are generated by protease action on kininogen, a phenomenon that is blocked by protease inhibitors. We evaluated kinin release in an in vivo lung inflammation model in rats, in the presence or absence of CeKI (C. echinata kallikrein inhibitor), a plasma kallikrein, cathepsin G, and proteinase-3 inhibitor, and rCeEI (recombinant C. echinata elastase inhibitor), which inhibits these proteases and also neutrophil elastase. Wistar rats were intravenously treated with buffer (negative control) or inhibitors and, subsequently, lipopolysaccharide was injected into their lungs. Blood, bronchoalveolar lavage fluid (BALF), and lung tissue were collected. In plasma, kinin release was higher in the LPS-treated animals in comparison to CeKI or rCeEI groups. rCeEI-treated animals presented less kinin than CeKI-treated group. Our data suggest that kinins play a pivotal role in lung inflammation and may be generated by different enzymes; however, neutrophil elastase seems to be the most important in the lung tissue context. These results open perspectives for a better understanding of biological process where neutrophil enzymes participate and indicate these plant inhibitors and their recombinant correlates for therapeutic trials involving pulmonary diseases.http://dx.doi.org/10.1155/2016/9425807 |
| spellingShingle | Ilana Cruz-Silva Viviane Abreu Nunes Andrezza Justino Gozzo Priscila Praxedes-Garcia Aparecida Sadae Tanaka Kazuaki Shimamoto Mariana Silva Araujo Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung Inflammation Pulmonary Medicine |
| title | Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung Inflammation |
| title_full | Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung Inflammation |
| title_fullStr | Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung Inflammation |
| title_full_unstemmed | Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung Inflammation |
| title_short | Protease Inhibitors Extracted from Caesalpinia echinata Lam. Affect Kinin Release during Lung Inflammation |
| title_sort | protease inhibitors extracted from caesalpinia echinata lam affect kinin release during lung inflammation |
| url | http://dx.doi.org/10.1155/2016/9425807 |
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