Depletion of transit amplifying cells in the adult brain does not affect quiescent neural stem cell pool size
Abstract Neural stem cells (NSCs) are maintained in the adult mammalian brain throughout the animal’s lifespan. NSCs in the subependymal zone infrequently divide and generate transit amplifying cells, which are destined to become olfactory bulb neurons. When transit amplifying cells are depleted, th...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
|
| Series: | Journal of Physiological Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12576-023-00876-2 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849304924085551104 |
|---|---|
| author | Zakiyyah Munirah Mohd Zaki Anri Kuroda Naoko Morimura Yoshitaka Hayashi Seiji Hitoshi |
| author_facet | Zakiyyah Munirah Mohd Zaki Anri Kuroda Naoko Morimura Yoshitaka Hayashi Seiji Hitoshi |
| author_sort | Zakiyyah Munirah Mohd Zaki |
| collection | DOAJ |
| description | Abstract Neural stem cells (NSCs) are maintained in the adult mammalian brain throughout the animal’s lifespan. NSCs in the subependymal zone infrequently divide and generate transit amplifying cells, which are destined to become olfactory bulb neurons. When transit amplifying cells are depleted, they are replenished by the quiescent NSC pool. However, the cellular basis for this recovery process remains largely unknown. In this study, we traced NSCs and their progeny after transit amplifying cells were eliminated by intraventricular infusion of cytosine β-D-arabinofuranoside. We found that although the number of neurosphere-forming NSCs decreased shortly after the treatment, they were restored to normal levels 3 weeks after the cessation of treatment. More importantly, the depletion of transit amplifying cells did not induce a significant expansion of the NSC pool by symmetric divisions. Our data suggest that the size of the NSC pool is hardly affected by brain damage due to antimitotic drug treatment. |
| format | Article |
| id | doaj-art-3afe810d9d804ff691a0a97565097b34 |
| institution | Kabale University |
| issn | 1880-6562 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Physiological Sciences |
| spelling | doaj-art-3afe810d9d804ff691a0a97565097b342025-08-20T03:55:36ZengElsevierJournal of Physiological Sciences1880-65622023-09-017311910.1186/s12576-023-00876-2Depletion of transit amplifying cells in the adult brain does not affect quiescent neural stem cell pool sizeZakiyyah Munirah Mohd Zaki0Anri Kuroda1Naoko Morimura2Yoshitaka Hayashi3Seiji Hitoshi4Department of Integrative Physiology, Shiga University of Medical ScienceDepartment of Integrative Physiology, Shiga University of Medical ScienceDepartment of Integrative Physiology, Shiga University of Medical ScienceDepartment of Integrative Physiology, Shiga University of Medical ScienceDepartment of Integrative Physiology, Shiga University of Medical ScienceAbstract Neural stem cells (NSCs) are maintained in the adult mammalian brain throughout the animal’s lifespan. NSCs in the subependymal zone infrequently divide and generate transit amplifying cells, which are destined to become olfactory bulb neurons. When transit amplifying cells are depleted, they are replenished by the quiescent NSC pool. However, the cellular basis for this recovery process remains largely unknown. In this study, we traced NSCs and their progeny after transit amplifying cells were eliminated by intraventricular infusion of cytosine β-D-arabinofuranoside. We found that although the number of neurosphere-forming NSCs decreased shortly after the treatment, they were restored to normal levels 3 weeks after the cessation of treatment. More importantly, the depletion of transit amplifying cells did not induce a significant expansion of the NSC pool by symmetric divisions. Our data suggest that the size of the NSC pool is hardly affected by brain damage due to antimitotic drug treatment.https://doi.org/10.1186/s12576-023-00876-2Lentiviral barcodingClonal analysisSubependymal zoneNeurospheresTransit amplifying cellAra-C |
| spellingShingle | Zakiyyah Munirah Mohd Zaki Anri Kuroda Naoko Morimura Yoshitaka Hayashi Seiji Hitoshi Depletion of transit amplifying cells in the adult brain does not affect quiescent neural stem cell pool size Journal of Physiological Sciences Lentiviral barcoding Clonal analysis Subependymal zone Neurospheres Transit amplifying cell Ara-C |
| title | Depletion of transit amplifying cells in the adult brain does not affect quiescent neural stem cell pool size |
| title_full | Depletion of transit amplifying cells in the adult brain does not affect quiescent neural stem cell pool size |
| title_fullStr | Depletion of transit amplifying cells in the adult brain does not affect quiescent neural stem cell pool size |
| title_full_unstemmed | Depletion of transit amplifying cells in the adult brain does not affect quiescent neural stem cell pool size |
| title_short | Depletion of transit amplifying cells in the adult brain does not affect quiescent neural stem cell pool size |
| title_sort | depletion of transit amplifying cells in the adult brain does not affect quiescent neural stem cell pool size |
| topic | Lentiviral barcoding Clonal analysis Subependymal zone Neurospheres Transit amplifying cell Ara-C |
| url | https://doi.org/10.1186/s12576-023-00876-2 |
| work_keys_str_mv | AT zakiyyahmunirahmohdzaki depletionoftransitamplifyingcellsintheadultbraindoesnotaffectquiescentneuralstemcellpoolsize AT anrikuroda depletionoftransitamplifyingcellsintheadultbraindoesnotaffectquiescentneuralstemcellpoolsize AT naokomorimura depletionoftransitamplifyingcellsintheadultbraindoesnotaffectquiescentneuralstemcellpoolsize AT yoshitakahayashi depletionoftransitamplifyingcellsintheadultbraindoesnotaffectquiescentneuralstemcellpoolsize AT seijihitoshi depletionoftransitamplifyingcellsintheadultbraindoesnotaffectquiescentneuralstemcellpoolsize |