A Structural Study of Escherichia coli Cells Using an In Situ Liquid Chamber TEM Technology
Studying cell microstructures and their behaviors under living conditions has been a challenging subject in microbiology. In this work, in situ liquid chamber TEM was used to study structures of Escherichia coli cells in aqueous solutions at a nanometer-scale resolution. Most of the cells remained i...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2015/829302 |
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| _version_ | 1849304923813969920 |
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| author | Yibing Wang Xin Chen Hongliang Cao Chao Deng Xiaodan Cao Ping Wang |
| author_facet | Yibing Wang Xin Chen Hongliang Cao Chao Deng Xiaodan Cao Ping Wang |
| author_sort | Yibing Wang |
| collection | DOAJ |
| description | Studying cell microstructures and their behaviors under living conditions has been a challenging subject in microbiology. In this work, in situ liquid chamber TEM was used to study structures of Escherichia coli cells in aqueous solutions at a nanometer-scale resolution. Most of the cells remained intact under electron beam irradiation, and nanoscale structures were observed during the TEM imaging. The analysis revealed structures of pili surrounding the E. coli cells; the movements of the pili in the liquid were also observed during the in situ tests. This technology also allowed the observation of features of the nucleoid in the E. coli cells. Overall, in situ TEM can be applied as a valuable tool to study real-time microscopic structures and processes in microbial cells residing in native aqueous solutions. |
| format | Article |
| id | doaj-art-3afe09e4d039495897eba8cdb3d962de |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-3afe09e4d039495897eba8cdb3d962de2025-08-20T03:55:36ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732015-01-01201510.1155/2015/829302829302A Structural Study of Escherichia coli Cells Using an In Situ Liquid Chamber TEM TechnologyYibing Wang0Xin Chen1Hongliang Cao2Chao Deng3Xiaodan Cao4Ping Wang5State Key Laboratory of Bioreactor Engineering, Biomedical Nanotechnology Center, East China University of Science and Technology, Shanghai 200237, ChinaKey Laboratory for Ultrafine Materials of Ministry of Education and Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, ChinaKey Laboratory for Ultrafine Materials of Ministry of Education and Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, ChinaKey Laboratory for Ultrafine Materials of Ministry of Education and Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, Biomedical Nanotechnology Center, East China University of Science and Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, Biomedical Nanotechnology Center, East China University of Science and Technology, Shanghai 200237, ChinaStudying cell microstructures and their behaviors under living conditions has been a challenging subject in microbiology. In this work, in situ liquid chamber TEM was used to study structures of Escherichia coli cells in aqueous solutions at a nanometer-scale resolution. Most of the cells remained intact under electron beam irradiation, and nanoscale structures were observed during the TEM imaging. The analysis revealed structures of pili surrounding the E. coli cells; the movements of the pili in the liquid were also observed during the in situ tests. This technology also allowed the observation of features of the nucleoid in the E. coli cells. Overall, in situ TEM can be applied as a valuable tool to study real-time microscopic structures and processes in microbial cells residing in native aqueous solutions.http://dx.doi.org/10.1155/2015/829302 |
| spellingShingle | Yibing Wang Xin Chen Hongliang Cao Chao Deng Xiaodan Cao Ping Wang A Structural Study of Escherichia coli Cells Using an In Situ Liquid Chamber TEM Technology Journal of Analytical Methods in Chemistry |
| title | A Structural Study of Escherichia coli Cells Using an In Situ Liquid Chamber TEM Technology |
| title_full | A Structural Study of Escherichia coli Cells Using an In Situ Liquid Chamber TEM Technology |
| title_fullStr | A Structural Study of Escherichia coli Cells Using an In Situ Liquid Chamber TEM Technology |
| title_full_unstemmed | A Structural Study of Escherichia coli Cells Using an In Situ Liquid Chamber TEM Technology |
| title_short | A Structural Study of Escherichia coli Cells Using an In Situ Liquid Chamber TEM Technology |
| title_sort | structural study of escherichia coli cells using an in situ liquid chamber tem technology |
| url | http://dx.doi.org/10.1155/2015/829302 |
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