Alteration of serotonin release response in the central nucleus of the amygdala to noxious and non-noxious mechanical stimulation in a neuropathic pain model rat

Abstract Previously, we found that serotonin (5-HT) release in the central nucleus of the amygdala (CeA) of anesthetized rats decreases in response to innocuous stroking of the skin, irrespective of stimulus laterality, but increases in response to noxious pinching applied to a hindlimb contralatera...

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Main Authors: Ryota Tokunaga, Hideshi Shibata, Mieko Kurosawa
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:Journal of Physiological Sciences
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Online Access:https://doi.org/10.1186/s12576-024-00910-x
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author Ryota Tokunaga
Hideshi Shibata
Mieko Kurosawa
author_facet Ryota Tokunaga
Hideshi Shibata
Mieko Kurosawa
author_sort Ryota Tokunaga
collection DOAJ
description Abstract Previously, we found that serotonin (5-HT) release in the central nucleus of the amygdala (CeA) of anesthetized rats decreases in response to innocuous stroking of the skin, irrespective of stimulus laterality, but increases in response to noxious pinching applied to a hindlimb contralateral to the 5-HT measurement site. The aim of the present study was to determine whether intra-CeA 5-HT release responses to cutaneous stimulation were altered in an animal model of neuropathic pain induced by ligation of the left L5 spinal nerve. In anesthetized neuropathic pain model rats, stroking of the left hindlimb increased 5-HT release in the CeA, whereas stroking of the right hindlimb decreased it. Meanwhile, pinching of the left hindlimb increased intra-CeA 5-HT release irrespective of stimulus laterality. In conclusion, the present study demonstrated that intra-CeA 5-HT release responses to cutaneous stimulation are altered in an animal model of neuropathic pain.
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spelling doaj-art-3aef990cdd6940aeaf3a502477d8cf602025-08-20T03:55:40ZengElsevierJournal of Physiological Sciences1880-65622024-03-017411810.1186/s12576-024-00910-xAlteration of serotonin release response in the central nucleus of the amygdala to noxious and non-noxious mechanical stimulation in a neuropathic pain model ratRyota Tokunaga0Hideshi Shibata1Mieko Kurosawa2Center for Medical Sciences, International University of Health and WelfareLaboratory of Veterinary Anatomy, Institute of Agriculture, Tokyo University of Agriculture and TechnologyCenter for Medical Sciences, International University of Health and WelfareAbstract Previously, we found that serotonin (5-HT) release in the central nucleus of the amygdala (CeA) of anesthetized rats decreases in response to innocuous stroking of the skin, irrespective of stimulus laterality, but increases in response to noxious pinching applied to a hindlimb contralateral to the 5-HT measurement site. The aim of the present study was to determine whether intra-CeA 5-HT release responses to cutaneous stimulation were altered in an animal model of neuropathic pain induced by ligation of the left L5 spinal nerve. In anesthetized neuropathic pain model rats, stroking of the left hindlimb increased 5-HT release in the CeA, whereas stroking of the right hindlimb decreased it. Meanwhile, pinching of the left hindlimb increased intra-CeA 5-HT release irrespective of stimulus laterality. In conclusion, the present study demonstrated that intra-CeA 5-HT release responses to cutaneous stimulation are altered in an animal model of neuropathic pain.https://doi.org/10.1186/s12576-024-00910-xNeuropathic painSerotonin releaseCentral nucleus of the amygdalaStrokingPinchingRat
spellingShingle Ryota Tokunaga
Hideshi Shibata
Mieko Kurosawa
Alteration of serotonin release response in the central nucleus of the amygdala to noxious and non-noxious mechanical stimulation in a neuropathic pain model rat
Journal of Physiological Sciences
Neuropathic pain
Serotonin release
Central nucleus of the amygdala
Stroking
Pinching
Rat
title Alteration of serotonin release response in the central nucleus of the amygdala to noxious and non-noxious mechanical stimulation in a neuropathic pain model rat
title_full Alteration of serotonin release response in the central nucleus of the amygdala to noxious and non-noxious mechanical stimulation in a neuropathic pain model rat
title_fullStr Alteration of serotonin release response in the central nucleus of the amygdala to noxious and non-noxious mechanical stimulation in a neuropathic pain model rat
title_full_unstemmed Alteration of serotonin release response in the central nucleus of the amygdala to noxious and non-noxious mechanical stimulation in a neuropathic pain model rat
title_short Alteration of serotonin release response in the central nucleus of the amygdala to noxious and non-noxious mechanical stimulation in a neuropathic pain model rat
title_sort alteration of serotonin release response in the central nucleus of the amygdala to noxious and non noxious mechanical stimulation in a neuropathic pain model rat
topic Neuropathic pain
Serotonin release
Central nucleus of the amygdala
Stroking
Pinching
Rat
url https://doi.org/10.1186/s12576-024-00910-x
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AT hideshishibata alterationofserotoninreleaseresponseinthecentralnucleusoftheamygdalatonoxiousandnonnoxiousmechanicalstimulationinaneuropathicpainmodelrat
AT miekokurosawa alterationofserotoninreleaseresponseinthecentralnucleusoftheamygdalatonoxiousandnonnoxiousmechanicalstimulationinaneuropathicpainmodelrat