Fasciola worm and egg-derived antigens: Exploring their diagnostic potential for urogenital schistosomiasis in resource-limited endemic regions
Objective: To evaluate the immunodiagnostic potential of crude Fasciola gigantica-worm (FWA) and egg antigen (FEA) in detecting anti-Schistosoma (S.) haematobium antibodies in sera and urine samples. Methods: This is a cross-sectional diagnostic study. Employing an indirect ELISA, antibodies against...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-11-01
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| Series: | Asian Pacific Journal of Tropical Medicine |
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| Online Access: | https://journals.lww.com/10.4103/apjtm.apjtm_80_24 |
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| author | Adedayo Adesida Tajudeen Oriade Kabirat Sulaiman Funmilayo Afolayan Timothy Auta Ibikunle Akanbi Mercy Aladegboye Roseangela Nwuba Alexander Odaibo Oyetunde Oyeyemi |
| author_facet | Adedayo Adesida Tajudeen Oriade Kabirat Sulaiman Funmilayo Afolayan Timothy Auta Ibikunle Akanbi Mercy Aladegboye Roseangela Nwuba Alexander Odaibo Oyetunde Oyeyemi |
| author_sort | Adedayo Adesida |
| collection | DOAJ |
| description | Objective:
To evaluate the immunodiagnostic potential of crude Fasciola gigantica-worm (FWA) and egg antigen (FEA) in detecting anti-Schistosoma (S.) haematobium antibodies in sera and urine samples.
Methods:
This is a cross-sectional diagnostic study. Employing an indirect ELISA, antibodies against these antigens were assessed in samples from infected and non-infected individuals in both schistosomiasis endemic (NE) and non-endemic (NNE) areas, using microscopy as the diagnostic standard.
Results:
FWA-sera exhibited excellent diagnostic accuracy with an area under the curve (AUC) of 0.957, a sensitivity of 93.75%, and a specificity of 85.42% for discriminating between infected and non-infected individuals in non-endemic areas. FWA-urine also demonstrated robust performance, achieving AUC>0.95, sensitivity>97.0%, and specificity>85.0% in both NE and NNE categories. Notably, S. haematobium-specific antibody levels against FWA were significantly elevated in infected individuals in both endemic and non-endemic areas. FEA-sera exhibited outstanding diagnostic performance with sensitivity exceeding 90% and an AUC of 0.968 in non-endemic samples but not in FEA-urine.
Conclusions:
FWA-based ELISAs, applicable to both sera and urine, emerge as promising tools for S. haematobium diagnosis in resource-limited settings, offering advantages of high sensitivity and specificity with shared antigens with Fasciola. The superior diagnostic metrics of urine samples suggest their potential as a noninvasive biological sample for diagnostic purposes. |
| format | Article |
| id | doaj-art-3adc2ba231ca43a4869cfce51fe75f38 |
| institution | OA Journals |
| issn | 2352-4146 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Medicine |
| spelling | doaj-art-3adc2ba231ca43a4869cfce51fe75f382025-08-20T02:30:38ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Medicine2352-41462024-11-01171150150710.4103/apjtm.apjtm_80_24Fasciola worm and egg-derived antigens: Exploring their diagnostic potential for urogenital schistosomiasis in resource-limited endemic regionsAdedayo AdesidaTajudeen OriadeKabirat SulaimanFunmilayo AfolayanTimothy AutaIbikunle AkanbiMercy AladegboyeRoseangela NwubaAlexander OdaiboOyetunde OyeyemiObjective: To evaluate the immunodiagnostic potential of crude Fasciola gigantica-worm (FWA) and egg antigen (FEA) in detecting anti-Schistosoma (S.) haematobium antibodies in sera and urine samples. Methods: This is a cross-sectional diagnostic study. Employing an indirect ELISA, antibodies against these antigens were assessed in samples from infected and non-infected individuals in both schistosomiasis endemic (NE) and non-endemic (NNE) areas, using microscopy as the diagnostic standard. Results: FWA-sera exhibited excellent diagnostic accuracy with an area under the curve (AUC) of 0.957, a sensitivity of 93.75%, and a specificity of 85.42% for discriminating between infected and non-infected individuals in non-endemic areas. FWA-urine also demonstrated robust performance, achieving AUC>0.95, sensitivity>97.0%, and specificity>85.0% in both NE and NNE categories. Notably, S. haematobium-specific antibody levels against FWA were significantly elevated in infected individuals in both endemic and non-endemic areas. FEA-sera exhibited outstanding diagnostic performance with sensitivity exceeding 90% and an AUC of 0.968 in non-endemic samples but not in FEA-urine. Conclusions: FWA-based ELISAs, applicable to both sera and urine, emerge as promising tools for S. haematobium diagnosis in resource-limited settings, offering advantages of high sensitivity and specificity with shared antigens with Fasciola. The superior diagnostic metrics of urine samples suggest their potential as a noninvasive biological sample for diagnostic purposes.https://journals.lww.com/10.4103/apjtm.apjtm_80_24schistosoma haematobiumnon-invasiveworm antigenelisaimmuno-diagnosis |
| spellingShingle | Adedayo Adesida Tajudeen Oriade Kabirat Sulaiman Funmilayo Afolayan Timothy Auta Ibikunle Akanbi Mercy Aladegboye Roseangela Nwuba Alexander Odaibo Oyetunde Oyeyemi Fasciola worm and egg-derived antigens: Exploring their diagnostic potential for urogenital schistosomiasis in resource-limited endemic regions Asian Pacific Journal of Tropical Medicine schistosoma haematobium non-invasive worm antigen elisa immuno-diagnosis |
| title | Fasciola worm and egg-derived antigens: Exploring their diagnostic potential for urogenital schistosomiasis in resource-limited endemic regions |
| title_full | Fasciola worm and egg-derived antigens: Exploring their diagnostic potential for urogenital schistosomiasis in resource-limited endemic regions |
| title_fullStr | Fasciola worm and egg-derived antigens: Exploring their diagnostic potential for urogenital schistosomiasis in resource-limited endemic regions |
| title_full_unstemmed | Fasciola worm and egg-derived antigens: Exploring their diagnostic potential for urogenital schistosomiasis in resource-limited endemic regions |
| title_short | Fasciola worm and egg-derived antigens: Exploring their diagnostic potential for urogenital schistosomiasis in resource-limited endemic regions |
| title_sort | fasciola worm and egg derived antigens exploring their diagnostic potential for urogenital schistosomiasis in resource limited endemic regions |
| topic | schistosoma haematobium non-invasive worm antigen elisa immuno-diagnosis |
| url | https://journals.lww.com/10.4103/apjtm.apjtm_80_24 |
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