Z/Ce@hemin enzymes with enhanced peroxidase activity for monitoring and screening the oxidative stress models of Parkinson’s disease
Abstract Inspired by natural enzymes, artificial enzymes have garnered significant interest due to their simplicity of production, robustness under harsh conditions, and enhanced stability. This study introduces, for the first time, a novel Z/Ce@hemin composite enzyme, constructed by anchoring hemin...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-03-01
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| Series: | npj Parkinson's Disease |
| Online Access: | https://doi.org/10.1038/s41531-025-00875-7 |
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| author | Li Lei Xiuli Chen Xincheng Long Qiuxia Tu Liping Zhao Dan Huang Lingli Song Jiaojiao Wang Suzhen Zhai Xiaozhong Chen Chunlin Zhang |
| author_facet | Li Lei Xiuli Chen Xincheng Long Qiuxia Tu Liping Zhao Dan Huang Lingli Song Jiaojiao Wang Suzhen Zhai Xiaozhong Chen Chunlin Zhang |
| author_sort | Li Lei |
| collection | DOAJ |
| description | Abstract Inspired by natural enzymes, artificial enzymes have garnered significant interest due to their simplicity of production, robustness under harsh conditions, and enhanced stability. This study introduces, for the first time, a novel Z/Ce@hemin composite enzyme, constructed by anchoring hemin onto zeolitic imidazolate framework-8 (ZIF-8)-encapsulated ceria (CeO2) nanoparticles. This innovative design overcomes the challenges of hemin dimerization, enhances substrate affinity, and accelerates mass transport, leading to significantly improved peroxidase-like activity. The enzyme also demonstrates remarkable stability and resistance to environmental interference. Utilizing this Z/Ce@hemin composite, a sensitive and selective colorimetric detection system was developed for rapid and accurate quantification of hydrogen peroxide (H2O2), a key oxidative stress marker. This approach was successfully applied in cellular models and Caenorhabditis elegans models of Parkinson’s disease, enabling precise monitoring of H2O2. The study provides a groundbreaking platform for oxidative stress modeling and expands possibilities in neurodegenerative disease research and therapeutic screening. |
| format | Article |
| id | doaj-art-3ad417f92e6e485fae840e82c161bf9c |
| institution | OA Journals |
| issn | 2373-8057 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Parkinson's Disease |
| spelling | doaj-art-3ad417f92e6e485fae840e82c161bf9c2025-08-20T02:16:34ZengNature Portfolionpj Parkinson's Disease2373-80572025-03-0111111010.1038/s41531-025-00875-7Z/Ce@hemin enzymes with enhanced peroxidase activity for monitoring and screening the oxidative stress models of Parkinson’s diseaseLi Lei0Xiuli Chen1Xincheng Long2Qiuxia Tu3Liping Zhao4Dan Huang5Lingli Song6Jiaojiao Wang7Suzhen Zhai8Xiaozhong Chen9Chunlin Zhang10Engineering Research Center for Molecular Medicine, School of Basic Medical Science, Guizhou Medical UniversityEngineering Research Center for Molecular Medicine, School of Basic Medical Science, Guizhou Medical UniversityEngineering Research Center for Molecular Medicine, School of Basic Medical Science, Guizhou Medical UniversityEngineering Research Center for Molecular Medicine, School of Basic Medical Science, Guizhou Medical UniversityEngineering Research Center for Molecular Medicine, School of Basic Medical Science, Guizhou Medical UniversityEngineering Research Center for Molecular Medicine, School of Basic Medical Science, Guizhou Medical UniversityEngineering Research Center for Molecular Medicine, School of Basic Medical Science, Guizhou Medical UniversityEngineering Research Center for Molecular Medicine, School of Basic Medical Science, Guizhou Medical UniversityEngineering Research Center for Molecular Medicine, School of Basic Medical Science, Guizhou Medical UniversityThe Jinyang Hospital Affiliated to Guizhou Medical University: The Second People’s Hospital of GuiyangEngineering Research Center for Molecular Medicine, School of Basic Medical Science, Guizhou Medical UniversityAbstract Inspired by natural enzymes, artificial enzymes have garnered significant interest due to their simplicity of production, robustness under harsh conditions, and enhanced stability. This study introduces, for the first time, a novel Z/Ce@hemin composite enzyme, constructed by anchoring hemin onto zeolitic imidazolate framework-8 (ZIF-8)-encapsulated ceria (CeO2) nanoparticles. This innovative design overcomes the challenges of hemin dimerization, enhances substrate affinity, and accelerates mass transport, leading to significantly improved peroxidase-like activity. The enzyme also demonstrates remarkable stability and resistance to environmental interference. Utilizing this Z/Ce@hemin composite, a sensitive and selective colorimetric detection system was developed for rapid and accurate quantification of hydrogen peroxide (H2O2), a key oxidative stress marker. This approach was successfully applied in cellular models and Caenorhabditis elegans models of Parkinson’s disease, enabling precise monitoring of H2O2. The study provides a groundbreaking platform for oxidative stress modeling and expands possibilities in neurodegenerative disease research and therapeutic screening.https://doi.org/10.1038/s41531-025-00875-7 |
| spellingShingle | Li Lei Xiuli Chen Xincheng Long Qiuxia Tu Liping Zhao Dan Huang Lingli Song Jiaojiao Wang Suzhen Zhai Xiaozhong Chen Chunlin Zhang Z/Ce@hemin enzymes with enhanced peroxidase activity for monitoring and screening the oxidative stress models of Parkinson’s disease npj Parkinson's Disease |
| title | Z/Ce@hemin enzymes with enhanced peroxidase activity for monitoring and screening the oxidative stress models of Parkinson’s disease |
| title_full | Z/Ce@hemin enzymes with enhanced peroxidase activity for monitoring and screening the oxidative stress models of Parkinson’s disease |
| title_fullStr | Z/Ce@hemin enzymes with enhanced peroxidase activity for monitoring and screening the oxidative stress models of Parkinson’s disease |
| title_full_unstemmed | Z/Ce@hemin enzymes with enhanced peroxidase activity for monitoring and screening the oxidative stress models of Parkinson’s disease |
| title_short | Z/Ce@hemin enzymes with enhanced peroxidase activity for monitoring and screening the oxidative stress models of Parkinson’s disease |
| title_sort | z ce hemin enzymes with enhanced peroxidase activity for monitoring and screening the oxidative stress models of parkinson s disease |
| url | https://doi.org/10.1038/s41531-025-00875-7 |
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