Odors drive feeding through gustatory receptor neurons in Drosophila

Odors are intimately tied to the taste system to aid food selection and determine the sensory experience of food. However, how smell and taste are integrated in the nervous system to drive feeding remains elusive. We show in Drosophila that odors alone activate gustatory receptor neurons (GRNs), tri...

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Main Authors: Hongping Wei, Thomas Ka Chung Lam, Hokto Kazama
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2025-08-01
Series:eLife
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Online Access:https://elifesciences.org/articles/101440
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author Hongping Wei
Thomas Ka Chung Lam
Hokto Kazama
author_facet Hongping Wei
Thomas Ka Chung Lam
Hokto Kazama
author_sort Hongping Wei
collection DOAJ
description Odors are intimately tied to the taste system to aid food selection and determine the sensory experience of food. However, how smell and taste are integrated in the nervous system to drive feeding remains elusive. We show in Drosophila that odors alone activate gustatory receptor neurons (GRNs), trigger proboscis extension reflex (PER), a canonical taste-evoked feeding behavior, and enhance food intake. Odor-evoked PER requires the function of sugar-sensing GRNs but not olfactory organs. Calcium imaging and electrophysiological recording show that GRNs directly respond to odors. Odor-evoked PER is mediated by the Gr5a receptor, and is bidirectionally modulated by olfactory binding proteins. Finally, odors and sucrose co-applied to GRNs synergistically enhance PER and food consumption. These results reveal a cell-intrinsic mechanism for odor-taste multimodal integration that takes place as early as in GRNs, indicating that unified chemosensory experience is a product of layered integration in peripheral neurons and in the brain.
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spelling doaj-art-3ad3efa3cfa44ad09677078bfd7b6e382025-08-20T03:22:12ZengeLife Sciences Publications LtdeLife2050-084X2025-08-011310.7554/eLife.101440Odors drive feeding through gustatory receptor neurons in DrosophilaHongping Wei0https://orcid.org/0000-0002-2910-8344Thomas Ka Chung Lam1https://orcid.org/0000-0002-4547-3245Hokto Kazama2https://orcid.org/0000-0002-3239-1873RIKEN Center for Brain Science, Saitama, JapanNeuroengineering Laboratory, Brain Mind Institute & Interfaculty Institute of Bioengineering, EPFL, Lausanne, SwitzerlandRIKEN Center for Brain Science, Saitama, Japan; RIKEN CBS-KAO Collaboration Center, Saitama, Japan; Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, JapanOdors are intimately tied to the taste system to aid food selection and determine the sensory experience of food. However, how smell and taste are integrated in the nervous system to drive feeding remains elusive. We show in Drosophila that odors alone activate gustatory receptor neurons (GRNs), trigger proboscis extension reflex (PER), a canonical taste-evoked feeding behavior, and enhance food intake. Odor-evoked PER requires the function of sugar-sensing GRNs but not olfactory organs. Calcium imaging and electrophysiological recording show that GRNs directly respond to odors. Odor-evoked PER is mediated by the Gr5a receptor, and is bidirectionally modulated by olfactory binding proteins. Finally, odors and sucrose co-applied to GRNs synergistically enhance PER and food consumption. These results reveal a cell-intrinsic mechanism for odor-taste multimodal integration that takes place as early as in GRNs, indicating that unified chemosensory experience is a product of layered integration in peripheral neurons and in the brain.https://elifesciences.org/articles/101440odor-taste multisensory integrationgustatory receptor neuronfeeding behaviorcalcium imagingtip recordingolfactory binding protein
spellingShingle Hongping Wei
Thomas Ka Chung Lam
Hokto Kazama
Odors drive feeding through gustatory receptor neurons in Drosophila
eLife
odor-taste multisensory integration
gustatory receptor neuron
feeding behavior
calcium imaging
tip recording
olfactory binding protein
title Odors drive feeding through gustatory receptor neurons in Drosophila
title_full Odors drive feeding through gustatory receptor neurons in Drosophila
title_fullStr Odors drive feeding through gustatory receptor neurons in Drosophila
title_full_unstemmed Odors drive feeding through gustatory receptor neurons in Drosophila
title_short Odors drive feeding through gustatory receptor neurons in Drosophila
title_sort odors drive feeding through gustatory receptor neurons in drosophila
topic odor-taste multisensory integration
gustatory receptor neuron
feeding behavior
calcium imaging
tip recording
olfactory binding protein
url https://elifesciences.org/articles/101440
work_keys_str_mv AT hongpingwei odorsdrivefeedingthroughgustatoryreceptorneuronsindrosophila
AT thomaskachunglam odorsdrivefeedingthroughgustatoryreceptorneuronsindrosophila
AT hoktokazama odorsdrivefeedingthroughgustatoryreceptorneuronsindrosophila