Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer
Photodynamic therapy (PDT) is a chemotherapeutic approach that utilizes a bifunctional reagent, a photosensitizer (PS) that localizes to the target tissue relative to the surrounding tissue and is toxic when exposed to laser light. PDT rapidly induces cell death, inflammatory and immune reactions, a...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Photoenergy |
| Online Access: | http://dx.doi.org/10.1155/2012/281068 |
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| _version_ | 1850217550948859904 |
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| author | A. El-Hussein M. Harith H. Abrahamse |
| author_facet | A. El-Hussein M. Harith H. Abrahamse |
| author_sort | A. El-Hussein |
| collection | DOAJ |
| description | Photodynamic therapy (PDT) is a chemotherapeutic approach that utilizes a bifunctional reagent, a photosensitizer (PS) that localizes to the target tissue relative to the surrounding tissue and is toxic when exposed to laser light. PDT rapidly induces cell death, inflammatory and immune reactions, and damage of the microvasculature. DNA damage results from a variety of factors including UV-light, X-rays, ionizing radiation, toxins, chemicals, or reactive oxygen species. The aim of this study was to determine the effect of PDT as well as the influence of presensitization leading to the adaptive response (AR) on the integrity of DNA. Lung (A549), breast (MCF-7), and esophageal (SNO) cancer cells and Zn sulfophthalocyanine as PS with irradiation conditions of 10 J/cm2 at 636 nm were used. Subcellular localization of PS, cell morphology, and viability after PDT and DNA damage were determined. A significant decrease in viability and marked DNA damage was observed in all 3 cancer cell types in response to PDT while the adaptive response was demonstrated to significantly decrease the effectiveness of the PDT. |
| format | Article |
| id | doaj-art-3ad20733f19a424a89c3f2965ed47cf5 |
| institution | OA Journals |
| issn | 1110-662X 1687-529X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Photoenergy |
| spelling | doaj-art-3ad20733f19a424a89c3f2965ed47cf52025-08-20T02:08:02ZengWileyInternational Journal of Photoenergy1110-662X1687-529X2012-01-01201210.1155/2012/281068281068Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine PhotosensitizerA. El-Hussein0M. Harith1H. Abrahamse2Laser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South AfricaNational Institute of Laser Enhanced Science, Cairo University, 12613 Giza, EgyptLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein 2028, South AfricaPhotodynamic therapy (PDT) is a chemotherapeutic approach that utilizes a bifunctional reagent, a photosensitizer (PS) that localizes to the target tissue relative to the surrounding tissue and is toxic when exposed to laser light. PDT rapidly induces cell death, inflammatory and immune reactions, and damage of the microvasculature. DNA damage results from a variety of factors including UV-light, X-rays, ionizing radiation, toxins, chemicals, or reactive oxygen species. The aim of this study was to determine the effect of PDT as well as the influence of presensitization leading to the adaptive response (AR) on the integrity of DNA. Lung (A549), breast (MCF-7), and esophageal (SNO) cancer cells and Zn sulfophthalocyanine as PS with irradiation conditions of 10 J/cm2 at 636 nm were used. Subcellular localization of PS, cell morphology, and viability after PDT and DNA damage were determined. A significant decrease in viability and marked DNA damage was observed in all 3 cancer cell types in response to PDT while the adaptive response was demonstrated to significantly decrease the effectiveness of the PDT.http://dx.doi.org/10.1155/2012/281068 |
| spellingShingle | A. El-Hussein M. Harith H. Abrahamse Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer International Journal of Photoenergy |
| title | Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer |
| title_full | Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer |
| title_fullStr | Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer |
| title_full_unstemmed | Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer |
| title_short | Assessment of DNA Damage after Photodynamic Therapy Using a Metallophthalocyanine Photosensitizer |
| title_sort | assessment of dna damage after photodynamic therapy using a metallophthalocyanine photosensitizer |
| url | http://dx.doi.org/10.1155/2012/281068 |
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