Internal changes associated with flight condition, reproductive maturity, and mating in bark and ambrosia beetles: A guide for dissection and assessment

Bark and ambrosia beetles are a diverse group which inhabit plants. Outbreaks of some species compete with human values and cause significant economic and ecological impacts. Identifying the flight condition, reproductive maturity, and mating status of individual bark beetles can benefit biological,...

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Bibliographic Details
Main Authors: Katherine P. Bleiker, Telsa Willsey, Gregory D. Smith
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:MethodsX
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S221501612500367X
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Summary:Bark and ambrosia beetles are a diverse group which inhabit plants. Outbreaks of some species compete with human values and cause significant economic and ecological impacts. Identifying the flight condition, reproductive maturity, and mating status of individual bark beetles can benefit biological, physiological, and behavioral studies. Here, we provide detailed methods to:Dissect the metathorax and abdomen of bark beetlesExtract and measure the lateral medius flight muscle and ovariolesExtract the spermatheca and assess the mating status of female bark beetles
ISSN:2215-0161