Kazakh Tobet dogs in the genomic landscape: refining the history of livestock guardian breeds

Abstract Background Despite the growing body of research on livestock guarding dog (LGD) breeds, Central Asian LGDs remain significantly underrepresented in genetic studies. Additionally, while some research has explored the genetic basis of selective traits in LGDs, significant gaps remain. By inve...

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Main Authors: Anastassiya Perfilyeva, Kira Bespalova, Yelena Kuzovleva, Mamura Begmanova, Almira Amirgaliyeva, Olga Vishnyakova, Inna Nazarenko, Sergey Bespalov, Assel Zhaxylykova, Arailym Yerzhan, Kanagat Yergali, Yuliya Perfilyeva, Rustam Mussabayev, Zhassulan Zhaniyazov
Format: Article
Language:English
Published: BMC 2025-08-01
Series:BMC Biology
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Online Access:https://doi.org/10.1186/s12915-025-02344-2
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author Anastassiya Perfilyeva
Kira Bespalova
Yelena Kuzovleva
Mamura Begmanova
Almira Amirgaliyeva
Olga Vishnyakova
Inna Nazarenko
Sergey Bespalov
Assel Zhaxylykova
Arailym Yerzhan
Kanagat Yergali
Yuliya Perfilyeva
Rustam Mussabayev
Zhassulan Zhaniyazov
author_facet Anastassiya Perfilyeva
Kira Bespalova
Yelena Kuzovleva
Mamura Begmanova
Almira Amirgaliyeva
Olga Vishnyakova
Inna Nazarenko
Sergey Bespalov
Assel Zhaxylykova
Arailym Yerzhan
Kanagat Yergali
Yuliya Perfilyeva
Rustam Mussabayev
Zhassulan Zhaniyazov
author_sort Anastassiya Perfilyeva
collection DOAJ
description Abstract Background Despite the growing body of research on livestock guarding dog (LGD) breeds, Central Asian LGDs remain significantly underrepresented in genetic studies. Additionally, while some research has explored the genetic basis of selective traits in LGDs, significant gaps remain. By investigating the genetic diversity, evolutionary history, and adaptive traits of the Kazakh Tobet, a Central Asian LGD breed from Kazakhstan, we aim to contribute to a deeper understanding of the genetic basis that characterizes LGD breeds. Results We analyzed the genetic relatedness of Kazakh Tobet dogs by combining SNP data from 90 unrelated Kazakh Tobets with publicly available SNP data from 124 dogs from 17 LGD breeds, 1753 dogs from 140 non-LGD breeds, 551 free-ranging dogs, and 16 wolves. The Kazakh Tobets exhibit considerable genetic diversity. Phylogenetic and PCA analyses suggest a common genetic origin with LGDs from Central and West Asia, which was complicated by gene flow from European LGDs. Signs of introgression from East Asian and Arctic non-LGDs may indicate a historical or modern gene exchange affecting the Kazakh Tobet lineage. Genetic clustering with free-ranging dogs supports the idea that open breeding systems do not compromise the functional integrity of LGD traits. Selection scans identified eight genomic regions with 127 genes associated with cognitive function, immune response, and metabolic regulation, suggesting the evolutionary importance of these traits in Kazakh Tobets. Conclusions This study provides new insights into the genetic history of LGD breeds and their connections to the broader genetic landscape of non-LGD breeds.
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spelling doaj-art-3abb8b26fd8640b493d659b6a09a27cf2025-08-20T03:06:05ZengBMCBMC Biology1741-70072025-08-0123112210.1186/s12915-025-02344-2Kazakh Tobet dogs in the genomic landscape: refining the history of livestock guardian breedsAnastassiya Perfilyeva0Kira Bespalova1Yelena Kuzovleva2Mamura Begmanova3Almira Amirgaliyeva4Olga Vishnyakova5Inna Nazarenko6Sergey Bespalov7Assel Zhaxylykova8Arailym Yerzhan9Kanagat Yergali10Yuliya Perfilyeva11Rustam Mussabayev12Zhassulan Zhaniyazov13Laboratory of Molecular Genetics, Institute of Genetics and PhysiologyLaboratory of Molecular Genetics, Institute of Genetics and PhysiologyLaboratory of Molecular Genetics, Institute of Genetics and PhysiologyLaboratory of Molecular Genetics, Institute of Genetics and PhysiologyLaboratory of Molecular Genetics, Institute of Genetics and PhysiologyDepartment of Cynology, Republican Federation of Public Associations of Hunters and Hunting Societies “Kansonar”Department of Cynology, Republican Federation of Public Associations of Hunters and Hunting Societies “Kansonar”Laboratory of Theriology, Institute of ZoologyLaboratory of Molecular Genetics, Institute of Genetics and PhysiologyLaboratory of Molecular Genetics, Institute of Genetics and PhysiologyLaboratory of Molecular Genetics, Institute of Genetics and PhysiologyLaboratory of Molecular Immunology and Immunobiotechnology, M.A. Aitkhozhin’s, Institute of Molecular Biology and BiochemistryLaboratory of Informational Processes Analysis and Modelling, Institute of Information and Computational TechnologiesLaboratory of Population Genetics, Institute of Genetics and PhysiologyAbstract Background Despite the growing body of research on livestock guarding dog (LGD) breeds, Central Asian LGDs remain significantly underrepresented in genetic studies. Additionally, while some research has explored the genetic basis of selective traits in LGDs, significant gaps remain. By investigating the genetic diversity, evolutionary history, and adaptive traits of the Kazakh Tobet, a Central Asian LGD breed from Kazakhstan, we aim to contribute to a deeper understanding of the genetic basis that characterizes LGD breeds. Results We analyzed the genetic relatedness of Kazakh Tobet dogs by combining SNP data from 90 unrelated Kazakh Tobets with publicly available SNP data from 124 dogs from 17 LGD breeds, 1753 dogs from 140 non-LGD breeds, 551 free-ranging dogs, and 16 wolves. The Kazakh Tobets exhibit considerable genetic diversity. Phylogenetic and PCA analyses suggest a common genetic origin with LGDs from Central and West Asia, which was complicated by gene flow from European LGDs. Signs of introgression from East Asian and Arctic non-LGDs may indicate a historical or modern gene exchange affecting the Kazakh Tobet lineage. Genetic clustering with free-ranging dogs supports the idea that open breeding systems do not compromise the functional integrity of LGD traits. Selection scans identified eight genomic regions with 127 genes associated with cognitive function, immune response, and metabolic regulation, suggesting the evolutionary importance of these traits in Kazakh Tobets. Conclusions This study provides new insights into the genetic history of LGD breeds and their connections to the broader genetic landscape of non-LGD breeds.https://doi.org/10.1186/s12915-025-02344-2Kazakh TobetLivestock guarding dogGenomic characterizationGenetic diversityPhylogenySignatures of selection
spellingShingle Anastassiya Perfilyeva
Kira Bespalova
Yelena Kuzovleva
Mamura Begmanova
Almira Amirgaliyeva
Olga Vishnyakova
Inna Nazarenko
Sergey Bespalov
Assel Zhaxylykova
Arailym Yerzhan
Kanagat Yergali
Yuliya Perfilyeva
Rustam Mussabayev
Zhassulan Zhaniyazov
Kazakh Tobet dogs in the genomic landscape: refining the history of livestock guardian breeds
BMC Biology
Kazakh Tobet
Livestock guarding dog
Genomic characterization
Genetic diversity
Phylogeny
Signatures of selection
title Kazakh Tobet dogs in the genomic landscape: refining the history of livestock guardian breeds
title_full Kazakh Tobet dogs in the genomic landscape: refining the history of livestock guardian breeds
title_fullStr Kazakh Tobet dogs in the genomic landscape: refining the history of livestock guardian breeds
title_full_unstemmed Kazakh Tobet dogs in the genomic landscape: refining the history of livestock guardian breeds
title_short Kazakh Tobet dogs in the genomic landscape: refining the history of livestock guardian breeds
title_sort kazakh tobet dogs in the genomic landscape refining the history of livestock guardian breeds
topic Kazakh Tobet
Livestock guarding dog
Genomic characterization
Genetic diversity
Phylogeny
Signatures of selection
url https://doi.org/10.1186/s12915-025-02344-2
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