The Fusion Gene <i>BPI-LY</i>, Encoding Human Bactericidal/Permeability-Increasing Protein Core Fragments and Lysozyme, Enhanced the Resistance of Transgenic Tomato Plants to Bacterial Wilt
Tomato bacterial wilt, caused by <i>Ralstonia solanacearum</i> (G<sup>−</sup>), is one of the most devastating plant diseases. Developing effective resistance against this pathogen remains a major challenge in plant disease management. In this study, we constructed a fusion g...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-06-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/13/1897 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849319768868257792 |
|---|---|
| author | Lei Ni Yue Zhang Yafei Qin Mei Wang Daodao Tang Liantian Chen Xing Ding Yilin Zheng Yu Pan Jinhua Li Xingguo Zhang |
| author_facet | Lei Ni Yue Zhang Yafei Qin Mei Wang Daodao Tang Liantian Chen Xing Ding Yilin Zheng Yu Pan Jinhua Li Xingguo Zhang |
| author_sort | Lei Ni |
| collection | DOAJ |
| description | Tomato bacterial wilt, caused by <i>Ralstonia solanacearum</i> (G<sup>−</sup>), is one of the most devastating plant diseases. Developing effective resistance against this pathogen remains a major challenge in plant disease management. In this study, we constructed a fusion gene <i>BPI-LY</i> by combining the gene encoding the lipophilic functional domains of human bactericidal/permeability-increasing protein (BPI) with the gene of human lysozyme (LY). The recombinant gene <i>BPI-LY</i> was heterologously expressed in yeast and tomato. Preliminary in vitro assays in yeast demonstrated that BPI enhances LY’s antibacterial activity against G<sup>−</sup> bacteria. Furthermore, overexpression of <i>BPI-LY</i> in tomato delayed onset of the disease in the transgenic lines and lowered the degree of tissue damage and the number of bacteria present in the stems relative to those in the wild-type plant. Additionally, the expression levels of the <i>SlSOD</i>, <i>SlPOD</i>, <i>SlPAL</i>, <i>SlPR5</i>, <i>SlPR10</i>, and <i>SlPR-NP24</i> genes were indirectly upregulated in the transgenic plants following <i>R. solanacearum</i> inoculation. Collectively, these findings demonstrate that <i>BPI-LY</i> enhances the resistance of transgenic tomato against bacterial wilt caused by <i>R. solanacearum</i>. |
| format | Article |
| id | doaj-art-3aba2ecbc289441b81bb3177ca1d630d |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-3aba2ecbc289441b81bb3177ca1d630d2025-08-20T03:50:20ZengMDPI AGPlants2223-77472025-06-011413189710.3390/plants14131897The Fusion Gene <i>BPI-LY</i>, Encoding Human Bactericidal/Permeability-Increasing Protein Core Fragments and Lysozyme, Enhanced the Resistance of Transgenic Tomato Plants to Bacterial WiltLei Ni0Yue Zhang1Yafei Qin2Mei Wang3Daodao Tang4Liantian Chen5Xing Ding6Yilin Zheng7Yu Pan8Jinhua Li9Xingguo Zhang10College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCollege of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaTomato bacterial wilt, caused by <i>Ralstonia solanacearum</i> (G<sup>−</sup>), is one of the most devastating plant diseases. Developing effective resistance against this pathogen remains a major challenge in plant disease management. In this study, we constructed a fusion gene <i>BPI-LY</i> by combining the gene encoding the lipophilic functional domains of human bactericidal/permeability-increasing protein (BPI) with the gene of human lysozyme (LY). The recombinant gene <i>BPI-LY</i> was heterologously expressed in yeast and tomato. Preliminary in vitro assays in yeast demonstrated that BPI enhances LY’s antibacterial activity against G<sup>−</sup> bacteria. Furthermore, overexpression of <i>BPI-LY</i> in tomato delayed onset of the disease in the transgenic lines and lowered the degree of tissue damage and the number of bacteria present in the stems relative to those in the wild-type plant. Additionally, the expression levels of the <i>SlSOD</i>, <i>SlPOD</i>, <i>SlPAL</i>, <i>SlPR5</i>, <i>SlPR10</i>, and <i>SlPR-NP24</i> genes were indirectly upregulated in the transgenic plants following <i>R. solanacearum</i> inoculation. Collectively, these findings demonstrate that <i>BPI-LY</i> enhances the resistance of transgenic tomato against bacterial wilt caused by <i>R. solanacearum</i>.https://www.mdpi.com/2223-7747/14/13/1897tomatobacterial wilt<i>Ralstonia solanacearum</i>bactericidal/permeability increasing protein and lysozyme fusion gene (<i>BPI-LY</i>)disease resistance |
| spellingShingle | Lei Ni Yue Zhang Yafei Qin Mei Wang Daodao Tang Liantian Chen Xing Ding Yilin Zheng Yu Pan Jinhua Li Xingguo Zhang The Fusion Gene <i>BPI-LY</i>, Encoding Human Bactericidal/Permeability-Increasing Protein Core Fragments and Lysozyme, Enhanced the Resistance of Transgenic Tomato Plants to Bacterial Wilt Plants tomato bacterial wilt <i>Ralstonia solanacearum</i> bactericidal/permeability increasing protein and lysozyme fusion gene (<i>BPI-LY</i>) disease resistance |
| title | The Fusion Gene <i>BPI-LY</i>, Encoding Human Bactericidal/Permeability-Increasing Protein Core Fragments and Lysozyme, Enhanced the Resistance of Transgenic Tomato Plants to Bacterial Wilt |
| title_full | The Fusion Gene <i>BPI-LY</i>, Encoding Human Bactericidal/Permeability-Increasing Protein Core Fragments and Lysozyme, Enhanced the Resistance of Transgenic Tomato Plants to Bacterial Wilt |
| title_fullStr | The Fusion Gene <i>BPI-LY</i>, Encoding Human Bactericidal/Permeability-Increasing Protein Core Fragments and Lysozyme, Enhanced the Resistance of Transgenic Tomato Plants to Bacterial Wilt |
| title_full_unstemmed | The Fusion Gene <i>BPI-LY</i>, Encoding Human Bactericidal/Permeability-Increasing Protein Core Fragments and Lysozyme, Enhanced the Resistance of Transgenic Tomato Plants to Bacterial Wilt |
| title_short | The Fusion Gene <i>BPI-LY</i>, Encoding Human Bactericidal/Permeability-Increasing Protein Core Fragments and Lysozyme, Enhanced the Resistance of Transgenic Tomato Plants to Bacterial Wilt |
| title_sort | fusion gene i bpi ly i encoding human bactericidal permeability increasing protein core fragments and lysozyme enhanced the resistance of transgenic tomato plants to bacterial wilt |
| topic | tomato bacterial wilt <i>Ralstonia solanacearum</i> bactericidal/permeability increasing protein and lysozyme fusion gene (<i>BPI-LY</i>) disease resistance |
| url | https://www.mdpi.com/2223-7747/14/13/1897 |
| work_keys_str_mv | AT leini thefusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT yuezhang thefusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT yafeiqin thefusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT meiwang thefusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT daodaotang thefusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT liantianchen thefusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT xingding thefusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT yilinzheng thefusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT yupan thefusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT jinhuali thefusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT xingguozhang thefusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT leini fusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT yuezhang fusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT yafeiqin fusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT meiwang fusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT daodaotang fusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT liantianchen fusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT xingding fusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT yilinzheng fusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT yupan fusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT jinhuali fusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt AT xingguozhang fusiongeneibpilyiencodinghumanbactericidalpermeabilityincreasingproteincorefragmentsandlysozymeenhancedtheresistanceoftransgenictomatoplantstobacterialwilt |