Sex‐Specific Effects of Social Environment on Behaviour and Their Correlations in Drosophila melanogaster

ABSTRACT Environmental and individual experiences can result in immediate and persistent changes in behaviour. Often, such effects are also sex‐dependent. Intraspecific interactions can be one of the most important environments an individual faces. Such social interactions are expected to affect a s...

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Main Authors: Erin L. Macartney, Samantha Burke, Patrice Pottier, Zina Hamoudi, Chloe Hart, Radiah Ahmed, Yong Qi Lin, G. Gregory Neely, Szymon M. Drobniak, Shinichi Nakagawa
Format: Article
Language:English
Published: Wiley 2025-04-01
Series:Ecology and Evolution
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Online Access:https://doi.org/10.1002/ece3.71261
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author Erin L. Macartney
Samantha Burke
Patrice Pottier
Zina Hamoudi
Chloe Hart
Radiah Ahmed
Yong Qi Lin
G. Gregory Neely
Szymon M. Drobniak
Shinichi Nakagawa
author_facet Erin L. Macartney
Samantha Burke
Patrice Pottier
Zina Hamoudi
Chloe Hart
Radiah Ahmed
Yong Qi Lin
G. Gregory Neely
Szymon M. Drobniak
Shinichi Nakagawa
author_sort Erin L. Macartney
collection DOAJ
description ABSTRACT Environmental and individual experiences can result in immediate and persistent changes in behaviour. Often, such effects are also sex‐dependent. Intraspecific interactions can be one of the most important environments an individual faces. Such social interactions are expected to affect a suite of behavioural traits and their correlations. Here, we used Drosophila melanogaster and high‐throughput automated behavioural phenotyping to determine how social environment (group mixed sex, group single sex, and social isolation) and sex interact to affect basic behaviours (exploration, movement within a y‐maze, and habituation to a startle) that likely underlie more complex behaviours such as mate searching and foraging. We show that such behaviours and some behavioural correlations are indeed context‐ and sex‐dependent. Males tended to show greater exploration, while females were more likely to show a habituation response to startle. Males and females from the mixed sex and isolated treatments showed opposite exploratory behaviour in the Y‐maze, and social treatment interacted with sex to affect the rate of habituation to a startle. Females also tended to have slightly stronger trait correlations compared to males. These results show that social environment and sex can play a significant role in shaping behaviour in Drosophila melanogaster. Our study provides insights into how the type of social stimulation and sex can interact to affect behaviours that are important in forming critical behaviours related to foraging and mate searching.
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spelling doaj-art-3ab6d18995b34ad181a5dfd667a3547d2025-08-20T03:32:11ZengWileyEcology and Evolution2045-77582025-04-01154n/an/a10.1002/ece3.71261Sex‐Specific Effects of Social Environment on Behaviour and Their Correlations in Drosophila melanogasterErin L. Macartney0Samantha Burke1Patrice Pottier2Zina Hamoudi3Chloe Hart4Radiah Ahmed5Yong Qi Lin6G. Gregory Neely7Szymon M. Drobniak8Shinichi Nakagawa9Evolution and Ecology Research Centre, School of Biological, Earth and Environmental Sciences University of New South Wales Sydney New South Wales AustraliaEvolution and Ecology Research Centre, School of Biological, Earth and Environmental Sciences University of New South Wales Sydney New South Wales AustraliaEvolution and Ecology Research Centre, School of Biological, Earth and Environmental Sciences University of New South Wales Sydney New South Wales AustraliaCharles Perkins Centre, School of Life and Environmental Sciences The University of Sydney Sydney New South Wales AustraliaCharles Perkins Centre, School of Life and Environmental Sciences The University of Sydney Sydney New South Wales AustraliaCharles Perkins Centre, School of Life and Environmental Sciences The University of Sydney Sydney New South Wales AustraliaCharles Perkins Centre, School of Life and Environmental Sciences The University of Sydney Sydney New South Wales AustraliaCharles Perkins Centre, School of Life and Environmental Sciences The University of Sydney Sydney New South Wales AustraliaEvolution and Ecology Research Centre, School of Biological, Earth and Environmental Sciences University of New South Wales Sydney New South Wales AustraliaEvolution and Ecology Research Centre, School of Biological, Earth and Environmental Sciences University of New South Wales Sydney New South Wales AustraliaABSTRACT Environmental and individual experiences can result in immediate and persistent changes in behaviour. Often, such effects are also sex‐dependent. Intraspecific interactions can be one of the most important environments an individual faces. Such social interactions are expected to affect a suite of behavioural traits and their correlations. Here, we used Drosophila melanogaster and high‐throughput automated behavioural phenotyping to determine how social environment (group mixed sex, group single sex, and social isolation) and sex interact to affect basic behaviours (exploration, movement within a y‐maze, and habituation to a startle) that likely underlie more complex behaviours such as mate searching and foraging. We show that such behaviours and some behavioural correlations are indeed context‐ and sex‐dependent. Males tended to show greater exploration, while females were more likely to show a habituation response to startle. Males and females from the mixed sex and isolated treatments showed opposite exploratory behaviour in the Y‐maze, and social treatment interacted with sex to affect the rate of habituation to a startle. Females also tended to have slightly stronger trait correlations compared to males. These results show that social environment and sex can play a significant role in shaping behaviour in Drosophila melanogaster. Our study provides insights into how the type of social stimulation and sex can interact to affect behaviours that are important in forming critical behaviours related to foraging and mate searching.https://doi.org/10.1002/ece3.71261behavioural plasticitybehavioural syndromeenvironmental enrichmentinsectmatingsex differences
spellingShingle Erin L. Macartney
Samantha Burke
Patrice Pottier
Zina Hamoudi
Chloe Hart
Radiah Ahmed
Yong Qi Lin
G. Gregory Neely
Szymon M. Drobniak
Shinichi Nakagawa
Sex‐Specific Effects of Social Environment on Behaviour and Their Correlations in Drosophila melanogaster
Ecology and Evolution
behavioural plasticity
behavioural syndrome
environmental enrichment
insect
mating
sex differences
title Sex‐Specific Effects of Social Environment on Behaviour and Their Correlations in Drosophila melanogaster
title_full Sex‐Specific Effects of Social Environment on Behaviour and Their Correlations in Drosophila melanogaster
title_fullStr Sex‐Specific Effects of Social Environment on Behaviour and Their Correlations in Drosophila melanogaster
title_full_unstemmed Sex‐Specific Effects of Social Environment on Behaviour and Their Correlations in Drosophila melanogaster
title_short Sex‐Specific Effects of Social Environment on Behaviour and Their Correlations in Drosophila melanogaster
title_sort sex specific effects of social environment on behaviour and their correlations in drosophila melanogaster
topic behavioural plasticity
behavioural syndrome
environmental enrichment
insect
mating
sex differences
url https://doi.org/10.1002/ece3.71261
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