Quantitative analysis of curcumin compounds in ginger by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry

Curcumin compounds are important bioactive compounds in ginger, yet their analysis is limited by their low concentrations. In the current research, a highly sensitive and reliable approach for simultaneous quantitative detection of three curcumin compounds in ginger samples was established using ult...

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Main Authors: Yixuan Jiang, Xiuhua Liu, Yinfeng Zhao, Jiguang Zhang, Jing Qiu, Yongzhong Qian, Rui Weng
Format: Article
Language:English
Published: Maximum Academic Press 2024-10-01
Series:Food Innovation and Advances
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Online Access:https://www.maxapress.com/article/doi/10.48130/fia-0024-0033
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author Yixuan Jiang
Xiuhua Liu
Yinfeng Zhao
Jiguang Zhang
Jing Qiu
Yongzhong Qian
Rui Weng
author_facet Yixuan Jiang
Xiuhua Liu
Yinfeng Zhao
Jiguang Zhang
Jing Qiu
Yongzhong Qian
Rui Weng
author_sort Yixuan Jiang
collection DOAJ
description Curcumin compounds are important bioactive compounds in ginger, yet their analysis is limited by their low concentrations. In the current research, a highly sensitive and reliable approach for simultaneous quantitative detection of three curcumin compounds in ginger samples was established using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). The extraction solvent, volume of extraction solvent, sonication time, and oscillation time were optimized by a single factor experiment. The method validation results showed that the regression coefficients were higher than 0.9990, and the linearity was satisfactory. Matrix effects were negligible with the values of 94.6%–98.8%. The recovery at three spiking levels was between 81.7% and 100.0%, and the precision was less than 5.4%. The approach could be used to determine the curcumin components in ginger samples since the results demonstrate that it is easy to use, practicable, repeatable, and accurate.
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id doaj-art-3aa1e9ecd5a14b8aaab2e0fd31f398d7
institution OA Journals
issn 2836-774X
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publishDate 2024-10-01
publisher Maximum Academic Press
record_format Article
series Food Innovation and Advances
spelling doaj-art-3aa1e9ecd5a14b8aaab2e0fd31f398d72025-08-20T02:12:31ZengMaximum Academic PressFood Innovation and Advances2836-774X2024-10-013435335910.48130/fia-0024-0033fia-0024-0033Quantitative analysis of curcumin compounds in ginger by ultra-high-performance liquid chromatography coupled with tandem mass spectrometryYixuan Jiang0Xiuhua Liu1Yinfeng Zhao2Jiguang Zhang3Jing Qiu4Yongzhong Qian5Rui Weng6Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaInstitute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaInstitute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaTobacco Research Institute, Chinese Academy of Agricultural Sciences, Laboratory of Risk Assessment for Tobacco Quality and Safety (Qingdao), Ministry of Agriculture and Rural Affairs, Qingdao 266101, ChinaInstitute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaInstitute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaInstitute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaCurcumin compounds are important bioactive compounds in ginger, yet their analysis is limited by their low concentrations. In the current research, a highly sensitive and reliable approach for simultaneous quantitative detection of three curcumin compounds in ginger samples was established using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). The extraction solvent, volume of extraction solvent, sonication time, and oscillation time were optimized by a single factor experiment. The method validation results showed that the regression coefficients were higher than 0.9990, and the linearity was satisfactory. Matrix effects were negligible with the values of 94.6%–98.8%. The recovery at three spiking levels was between 81.7% and 100.0%, and the precision was less than 5.4%. The approach could be used to determine the curcumin components in ginger samples since the results demonstrate that it is easy to use, practicable, repeatable, and accurate.https://www.maxapress.com/article/doi/10.48130/fia-0024-0033curcumindemethoxycurcuminbisdemethoxycurcuminuhplc-ms/msginger
spellingShingle Yixuan Jiang
Xiuhua Liu
Yinfeng Zhao
Jiguang Zhang
Jing Qiu
Yongzhong Qian
Rui Weng
Quantitative analysis of curcumin compounds in ginger by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry
Food Innovation and Advances
curcumin
demethoxycurcumin
bisdemethoxycurcumin
uhplc-ms/ms
ginger
title Quantitative analysis of curcumin compounds in ginger by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry
title_full Quantitative analysis of curcumin compounds in ginger by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry
title_fullStr Quantitative analysis of curcumin compounds in ginger by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry
title_full_unstemmed Quantitative analysis of curcumin compounds in ginger by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry
title_short Quantitative analysis of curcumin compounds in ginger by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry
title_sort quantitative analysis of curcumin compounds in ginger by ultra high performance liquid chromatography coupled with tandem mass spectrometry
topic curcumin
demethoxycurcumin
bisdemethoxycurcumin
uhplc-ms/ms
ginger
url https://www.maxapress.com/article/doi/10.48130/fia-0024-0033
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