Pretreatment method of forchlorfenuron residues in loquat by HPLC analysis
Forchlorfenuron,1-(2-chloro-4-pyridyl)-3-phenyl, is a multipurpose plant growth regulator. The previous reports showed that forchlorfenuron was a relatively safe and low-toxic pesticide without teratogenic and mutagenic effects on human and animals. However, recent researches showed that excessive e...
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Zhejiang University Press
2016-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2015.11.101 |
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| author | YANG Chunping LI Zhaojun NING Hong CHEN Huabao GONG Guoshu YANG Jizhi ZHANG Min |
| author_facet | YANG Chunping LI Zhaojun NING Hong CHEN Huabao GONG Guoshu YANG Jizhi ZHANG Min |
| author_sort | YANG Chunping |
| collection | DOAJ |
| description | Forchlorfenuron,1-(2-chloro-4-pyridyl)-3-phenyl, is a multipurpose plant growth regulator. The previous reports showed that forchlorfenuron was a relatively safe and low-toxic pesticide without teratogenic and mutagenic effects on human and animals. However, recent researches showed that excessive exposure of forchlorfenuron could cause the disorder of protein metabolism, mild emphysema. Therefore, residue detection of forchlorfenuron in fruits and vegetables is imperative.High-performance liquid chromatography (HPLC) analysis is a common detection method. The suitable conditions for the extraction and purification of sample play an important role prior to HPLC analysis.In this study, the extraction efficiency of forchlorfenuron residues in loquat was compared using matrix dispersive solid phase extraction and QuEChERS (quick, easy, cheap, effective, rugged, safe).The results showed that QuEChERS was a more suitable pretreatment method for the determination of forchlorfenuron residues in loquat, because it had obviously higher average recoveries than the matrix dispersive solid phase extraction. Effects of different extraction and purification conditions on QuEChERS were further studied. The optimal conditions obtained were that 8.0 g of loquat samples were extracted with 16 mL of acetonitrile for 20 min, and then salted out for dehydration by m (anhydrous magnesium sulfate)∶m (sodium chloride)=3∶2, and ultimately purified by m (primary secondary amine (PSA))∶m (C<sub>18</sub>)=1∶1 for 3 min. The forchlorfenuron residues in loquat could be completely separated from impurities under these optimal conditions, and furthermore, the spiked recoveries of forchlorfenuron in loquat were 80%-100%, with coefficient of variation (CV) in the range of 3.1%-4.8%.It was concluded that our method of pretreatment could effectively extract and purify forchlorfenuron residues in loquat. |
| format | Article |
| id | doaj-art-3a9cc04bcd9e4a05a16952626c928dd0 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2016-05-01 |
| publisher | Zhejiang University Press |
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| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-3a9cc04bcd9e4a05a16952626c928dd02025-08-20T03:32:07ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552016-05-014235035710.3785/j.issn.1008-9209.2015.11.10110089209Pretreatment method of forchlorfenuron residues in loquat by HPLC analysisYANG ChunpingLI ZhaojunNING HongCHEN HuabaoGONG GuoshuYANG JizhiZHANG MinForchlorfenuron,1-(2-chloro-4-pyridyl)-3-phenyl, is a multipurpose plant growth regulator. The previous reports showed that forchlorfenuron was a relatively safe and low-toxic pesticide without teratogenic and mutagenic effects on human and animals. However, recent researches showed that excessive exposure of forchlorfenuron could cause the disorder of protein metabolism, mild emphysema. Therefore, residue detection of forchlorfenuron in fruits and vegetables is imperative.High-performance liquid chromatography (HPLC) analysis is a common detection method. The suitable conditions for the extraction and purification of sample play an important role prior to HPLC analysis.In this study, the extraction efficiency of forchlorfenuron residues in loquat was compared using matrix dispersive solid phase extraction and QuEChERS (quick, easy, cheap, effective, rugged, safe).The results showed that QuEChERS was a more suitable pretreatment method for the determination of forchlorfenuron residues in loquat, because it had obviously higher average recoveries than the matrix dispersive solid phase extraction. Effects of different extraction and purification conditions on QuEChERS were further studied. The optimal conditions obtained were that 8.0 g of loquat samples were extracted with 16 mL of acetonitrile for 20 min, and then salted out for dehydration by m (anhydrous magnesium sulfate)∶m (sodium chloride)=3∶2, and ultimately purified by m (primary secondary amine (PSA))∶m (C<sub>18</sub>)=1∶1 for 3 min. The forchlorfenuron residues in loquat could be completely separated from impurities under these optimal conditions, and furthermore, the spiked recoveries of forchlorfenuron in loquat were 80%-100%, with coefficient of variation (CV) in the range of 3.1%-4.8%.It was concluded that our method of pretreatment could effectively extract and purify forchlorfenuron residues in loquat.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2015.11.101loquatforchlorfenuronresidue analysishigh efficiency liquid chromatographymatrix dispersive solid phase extractionQuEChERS |
| spellingShingle | YANG Chunping LI Zhaojun NING Hong CHEN Huabao GONG Guoshu YANG Jizhi ZHANG Min Pretreatment method of forchlorfenuron residues in loquat by HPLC analysis 浙江大学学报. 农业与生命科学版 loquat forchlorfenuron residue analysis high efficiency liquid chromatography matrix dispersive solid phase extraction QuEChERS |
| title | Pretreatment method of forchlorfenuron residues in loquat by HPLC analysis |
| title_full | Pretreatment method of forchlorfenuron residues in loquat by HPLC analysis |
| title_fullStr | Pretreatment method of forchlorfenuron residues in loquat by HPLC analysis |
| title_full_unstemmed | Pretreatment method of forchlorfenuron residues in loquat by HPLC analysis |
| title_short | Pretreatment method of forchlorfenuron residues in loquat by HPLC analysis |
| title_sort | pretreatment method of forchlorfenuron residues in loquat by hplc analysis |
| topic | loquat forchlorfenuron residue analysis high efficiency liquid chromatography matrix dispersive solid phase extraction QuEChERS |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2015.11.101 |
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