Mitochondrial phosphate carrier plays an important role in virulence of Candida albicans
Candida albicans is a common fungal pathogen that can cause life-threatening infections. MIR1 is considered to be a mitochondrial phosphate carrier of C. albicans, while its role in virulence has not been fully elucidated. In this study, we found that mir1Δ/Δ mutant exhibited severe virulence defect...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-01-01
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| Series: | Mycology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21501203.2024.2354876 |
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| author | Qiao-Ling Hu Hua Zhong Xin-Rong Wang Lei Han Shan-Shan Ma Ling Li Yan Wang |
| author_facet | Qiao-Ling Hu Hua Zhong Xin-Rong Wang Lei Han Shan-Shan Ma Ling Li Yan Wang |
| author_sort | Qiao-Ling Hu |
| collection | DOAJ |
| description | Candida albicans is a common fungal pathogen that can cause life-threatening infections. MIR1 is considered to be a mitochondrial phosphate carrier of C. albicans, while its role in virulence has not been fully elucidated. In this study, we found that mir1Δ/Δ mutant exhibited severe virulence defect in both nematode and murine models. Further mechanism studies revealed that the mir1Δ/Δ mutant grew more slowly than the wild-type strain and showed severe filamentation defects on the hypha-inducing agar media, including YPD + serum, Lee, Spider + glucose, SLAD, SLD, and YPS. Furthermore, the loss of MIR1 resulted in unfermentable carbon utilisation defect, ATP decrease, and reactive oxygen species (ROS) accumulation in C. albicans. Antioxidant proanthocyanidins, vitamin E, and N-acetyl cysteine (NAC) could reduce intracellular ROS levels and partially rescue the filamentation defects of the mir1Δ/Δ mutant. Accordingly, hypha-specific genes, as well as CEK1 and RIM101 were down-regulated in the mir1Δ/Δ mutant, and this down-regulation could be partially rescued by the addition of the antioxidant NAC. Collectively, MIR1 plays an important role in C. albicans mitochondrial function, filamentation and virulence, and would be a promising antifungal target. |
| format | Article |
| id | doaj-art-3a935ca75bc7445681d2602b90359fd6 |
| institution | OA Journals |
| issn | 2150-1203 2150-1211 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycology |
| spelling | doaj-art-3a935ca75bc7445681d2602b90359fd62025-08-20T02:29:55ZengTaylor & Francis GroupMycology2150-12032150-12112025-01-0116136938110.1080/21501203.2024.2354876Mitochondrial phosphate carrier plays an important role in virulence of Candida albicansQiao-Ling Hu0Hua Zhong1Xin-Rong Wang2Lei Han3Shan-Shan Ma4Ling Li5Yan Wang6School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, ChinaSchool of Pharmacy, Second Military Medical University (Naval Medical University), Shanghai, ChinaSchool of Pharmacy, Second Military Medical University (Naval Medical University), Shanghai, ChinaSchool of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, ChinaSchool of Pharmacy, Second Military Medical University (Naval Medical University), Shanghai, ChinaSchool of Pharmacy, Second Military Medical University (Naval Medical University), Shanghai, ChinaSchool of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, ChinaCandida albicans is a common fungal pathogen that can cause life-threatening infections. MIR1 is considered to be a mitochondrial phosphate carrier of C. albicans, while its role in virulence has not been fully elucidated. In this study, we found that mir1Δ/Δ mutant exhibited severe virulence defect in both nematode and murine models. Further mechanism studies revealed that the mir1Δ/Δ mutant grew more slowly than the wild-type strain and showed severe filamentation defects on the hypha-inducing agar media, including YPD + serum, Lee, Spider + glucose, SLAD, SLD, and YPS. Furthermore, the loss of MIR1 resulted in unfermentable carbon utilisation defect, ATP decrease, and reactive oxygen species (ROS) accumulation in C. albicans. Antioxidant proanthocyanidins, vitamin E, and N-acetyl cysteine (NAC) could reduce intracellular ROS levels and partially rescue the filamentation defects of the mir1Δ/Δ mutant. Accordingly, hypha-specific genes, as well as CEK1 and RIM101 were down-regulated in the mir1Δ/Δ mutant, and this down-regulation could be partially rescued by the addition of the antioxidant NAC. Collectively, MIR1 plays an important role in C. albicans mitochondrial function, filamentation and virulence, and would be a promising antifungal target.https://www.tandfonline.com/doi/10.1080/21501203.2024.2354876Candida albicansMIR1reactive oxygen speciesfilamentationvirulence |
| spellingShingle | Qiao-Ling Hu Hua Zhong Xin-Rong Wang Lei Han Shan-Shan Ma Ling Li Yan Wang Mitochondrial phosphate carrier plays an important role in virulence of Candida albicans Mycology Candida albicans MIR1 reactive oxygen species filamentation virulence |
| title | Mitochondrial phosphate carrier plays an important role in virulence of Candida albicans |
| title_full | Mitochondrial phosphate carrier plays an important role in virulence of Candida albicans |
| title_fullStr | Mitochondrial phosphate carrier plays an important role in virulence of Candida albicans |
| title_full_unstemmed | Mitochondrial phosphate carrier plays an important role in virulence of Candida albicans |
| title_short | Mitochondrial phosphate carrier plays an important role in virulence of Candida albicans |
| title_sort | mitochondrial phosphate carrier plays an important role in virulence of candida albicans |
| topic | Candida albicans MIR1 reactive oxygen species filamentation virulence |
| url | https://www.tandfonline.com/doi/10.1080/21501203.2024.2354876 |
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